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101.
用人工合成的丁型肝炎病毒抗原(HDV-Ag)肽建立了检测抗HDV-IgM抗体的ELISA方法,本法操作简便、快速,重复性好,特异性强,与抗HAV-IgM、抗Hk-IgM、抗HBs-IgM、抗HCV-IgI、抗CMV-IgM、抗RV-IgM、类风湿因子(RF)及抗核抗体(ANA)阳性血清均不起反应,且可被2-巯基乙醇阻断而不起反应。经初步临床应用,31例正常人血清抗HDV-IgM全部阴性,28例慢活肝患者检出率为32.1%(9/28),17例慢迁肝患者血清阳性率为11.8%(2/17)18例肝癌和肝硬化病人血清阳性率为22.2%(4/18)这三组病人与正常对照者相比较均有显著性差异(P<0.001)。此外,抗HDV-IgM阳性血清的ALT值均明显高于正常参考范围,提示在HDV感染过程中,患者肝细胞进一步受损。实验结果证明,抗HDV-IgM是诊断HDV感染的重要指标,对HDV感染早期诊断具有重要价值。 相似文献
102.
武汉东湖水生植被及其恢复途径探讨 总被引:4,自引:0,他引:4
1992~1993年对武汉东湖三个主要湖区(郭郑湖、汤林湖和牛巢湖)水生植被的调查表明,该湖区共有水生植物32种,优势种为大茨藻、狐尾藻、苦草和菱。金鱼藻呈不断扩大的趋势。植被类型可分为11个群丛,植被面积约为0.65km ̄2,总生物量为1236.39t(湿重),植被带状分布仅见于汤林湖北部和其他部分湖汊。汤林湖和牛巢湖水生植被正处于自然恢复演替阶段。 相似文献
103.
Françoise Mead Catherine Habersetzer Danielle Gabouriaut Jacques Gervet 《Journal of Ethology》1994,12(1):43-51
This study focused on how a decrease in prey availability affected the development of aP. dominulus Christ colony. Nutritional oophagy and larval development were parameters found to be most directly affected. The more indirect
effects on the growth of the nest and on offspring production were also analyzed. 相似文献
104.
Prof. Danielle Swinne 《Mycopathologia》1994,128(2):65-65
Editor's Note. Dr Vanbreuseghem was a member of the Mycopathologia Educational Board from 1959 to 1960. 相似文献
105.
D. K. X. Chong C. C. Chinnappa F. C. Yeh S. Chuong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(5):614-617
Inheritance of chloroplast DNA (cpDNA) was examined in F1 progenies derived from three crosses and three corresponding reciprocal crosses betweenStellaria porsildii andS. longifolia. Chloroplast DNA restriction fragments were analyzed using methods of nonradioactive digoxigenin-11-dUTP labeling and chemiluminescent detection with Lumi-Phos 530. Distinct interspecific restriction fragment polymorphisms were identified and used to demonstrate the mode of cpDNA inheritance. Mode of cpDNA inheritance differed among crosses. Two crosses in whichS. porsildii, SP2920-21, was the maternal parent exhibited three different types of plastids, maternal, paternal and biparental, among the F1 hybrids, suggesting a biparental cpDNA inheritance and plastid sorting-out inStellaria. 相似文献
106.
J. R. Smith S. A. Leong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(8):901-908
Magnaporthe grisea causes rice blast, the most important fungal disease of rice. The segregation of genes controlling virulence of M. grisea on rice was studied to establish the genetic basis of cultivar specificity in this host-parasite interaction. Full-sib progeny and parent isolates Guy11 and 2539 of M. grisea were inoculated onto rice (Oryza sativa) cultivar CO39 and five near-isogenic lines (NILs) of CO39. Each NIL contained a different single gene affecting resistance to specific isolates of M. grisea. No differential interactions between NILs and progeny or parents were observed; parents and progeny pathogenic on CO39 were pathogenic on all five NILs. Segregation ratios of 101 full-sib progeny, 117 progeny from full-sib parents, and 109 backcross progeny, indicated a common single gene affecting pathogenicity on CO39 and the five NILs. A subset of the above 327 isolates (43 fullsib progeny, 37 progeny from full-sib parents, and 32 backcross progeny) were inoculated onto rice cultivar 51583; all were pathogenic, indicating that cultivar specificity to CO39 was segregating in this population of isolates. The locus controlling cultivar specificity, named avrCO39, was mapped to chromosome 1 using a subset of the progeny previously used to construct an RFLP map of M. grisea. The closest reported RFLP markers were 11.8 (estimated 260 kb) and 17.2 cM (estimated 380 kb) away and provide starting points on either side of the locus for a chromosome walk to clone the locus. 相似文献
107.
Kow Jen Duan Dey Chyi Sheu Ming Tse Lin Hsiao Chiang Hsueh 《Biotechnology letters》1994,16(11):1151-1156
Summary An -glucosidase fromAspergillus carbonarious CCRC 30414 was employed for investigating the enzymatic synthesis of isomaltooligosaccharides from maltose. The enzyme transferred a glucose unit from the nonreducing end of maltose and other -linked glucosyl oligosaccharides to glucose and other glucosyl oligosaccharides which function as accepting co-substrates. The transfer of a glucose unit occurs most frequently to the 6-OH position of the nonreducing end of acceptor, but transfer to 4-OH position also occurs. Treatment of 30 % (w/v) maltose with the enzyme under optimum conditions afforded more than 50% isomaltooligosaccharides. 相似文献
108.
109.
横纹肌肌原纤维的第三肌丝──肌联蛋白 总被引:2,自引:0,他引:2
陈明 《生物化学与生物物理进展》1994,21(5):403-406
实验研究证明,在动物横纹肌肌原纤维中,除包含有粗肌丝、细肌丝外,还有纤肌丝的存在,肌联蛋白(肌巨蛋白)是具有挠性的线状蛋白质,分子量为3000 000,长度约为0.9μm,跨越肌原纤维的M-线和Z-线,形成纤肌丝.其生理功能是在粗肌丝装配中具有分子模板作用,并将粗肌丝稳定于肌原纤维肌小节中央以及可参与肌球蛋白活性的调节. 相似文献
110.
Anthony T.W. Cheung Richard B. Moss Geoffrey Kurland Albin B. Leong William J. Novick 《Journal of medical primatology》1993,22(4):257-262
We have recently established a rhesus monkey model of chronic Pseudomonas aeruginosa (PA) endobronchitis by bronchoscopic instillation of PA-embedded agar beads. All experimental animals developed chronic neutrophilic endobronchitis similar to chronic PA endobronchitis in cystic fibrosis (CF). Histopathologic studies further confirmed similarities to chronic PA endobronchitis in CF, including marked peribronchial inflammation, epithelial damage, presence of degraded cilia and ciliary abnormalities, appearance of PA bacterial clusters, mucosal hyperplasia, goblet cell hypertrophy/hypersecretion, airway obstruction, alveolar abnormalities, bronchiectasis, and fibrosis. 相似文献