全文获取类型
收费全文 | 22869篇 |
免费 | 2130篇 |
国内免费 | 2222篇 |
出版年
2024年 | 42篇 |
2023年 | 273篇 |
2022年 | 536篇 |
2021年 | 1099篇 |
2020年 | 805篇 |
2019年 | 1031篇 |
2018年 | 986篇 |
2017年 | 712篇 |
2016年 | 1055篇 |
2015年 | 1504篇 |
2014年 | 1852篇 |
2013年 | 1781篇 |
2012年 | 2214篇 |
2011年 | 2013篇 |
2010年 | 1301篇 |
2009年 | 1107篇 |
2008年 | 1388篇 |
2007年 | 1289篇 |
2006年 | 1012篇 |
2005年 | 905篇 |
2004年 | 737篇 |
2003年 | 673篇 |
2002年 | 603篇 |
2001年 | 360篇 |
2000年 | 317篇 |
1999年 | 327篇 |
1998年 | 217篇 |
1997年 | 172篇 |
1996年 | 127篇 |
1995年 | 99篇 |
1994年 | 110篇 |
1993年 | 63篇 |
1992年 | 92篇 |
1991年 | 70篇 |
1990年 | 55篇 |
1989年 | 52篇 |
1988年 | 38篇 |
1987年 | 41篇 |
1986年 | 27篇 |
1985年 | 31篇 |
1984年 | 20篇 |
1983年 | 13篇 |
1982年 | 10篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1965年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 421 毫秒
11.
“Candidatus Liberibacter asiaticus” (CLas) is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature. This study began with the genome sequence analysis of CLas Strain A4 from Guangdong in the prophage region. Within the two currently known prophage types, Type 1 (SC1-like) and Type 2 (SC2-like), A4 genome contained only a Type 2 prophage, CGdP2, namely. An analysis on CLas strains collected in Guangdong showed that Type 2 prophage dominated the bacterial population (82.6%, 71/86). An extended survey covering five provinces in southern China also revealed the predominance of single prophage (Type 1 or Type 2) in the CLas population (90.4%, 169/187). CLas strains with two and no prophage types accounted for 7.2% and 2.8%, respectively. In silico analyses on CGdP2 identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) system, consisting of four 22 bp repeats, three 23 bp spacers and 9 predicted cas. Similar CRISPR/cas systems were detected in all 10 published CLas prophages as well as 13 CLas field strains in southern China. Both Type 1 and Type 2 prophages shared almost identical sequences in spacer 1 and 3 but not spacer 2. Considering that the function of a CRISPR/cas system was to destroy invading DNA, it was hypothesized that a pre-established CLas prophage could use its CRISPR/cas system guided by spacer 1 and/or 3 to defeat the invasion of the other phage/prophage. This hypothesis explained the predominance of single prophage type in the CLas population in southern China. This is the first report of CRISPR/cas system in the “Ca. Liberibacter” genera. 相似文献
12.
Xin Deng Guoli Zhang Ling Zhang Yan Feng Zehong Li GuangMou Wu Yuhuan Yue Gensong Li Yu Cao Ping Zhu 《PloS one》2015,10(11)
Non-viral gene delivery system with many advantages has a great potential for the future of gene therapy. One inherent obstacle of such approach is the uptake by endocytosis into vesicular compartments. Receptor-mediated gene delivery method holds promise to overcome this obstacle. In this study, we developed a receptor-mediated gene delivery system based on a combination of the Pseudomonas exotoxin A (PE), which has a receptor binding and membrane translocation domain, and the hyperthermophilic archaeal histone (HPhA), which has the DNA binding ability. First, we constructed and expressed the rPE-HPhA fusion protein. We then examined the cytotoxicity and the DNA binding ability of rPE-HPhA. We further assessed the efficiency of transfection of the pEGF-C1 plasmid DNA to CHO cells by the rPE-HPhA system, in comparison to the cationic liposome method. The results showed that the transfection efficiency of rPE-HPhA was higher than that of cationic liposomes. In addition, the rPE-HPhA gene delivery system is non-specific to DNA sequence, topology or targeted cell type. Thus, the rPE-HPhA system can be used for delivering genes of interest into mammalian cells and has great potential to be applied for gene therapy. 相似文献
13.
Christine E. Brown Charles D. Warden Renate Starr Xutao Deng Behnam Badie Yate-Ching Yuan Stephen J. Forman Michael E. Barish 《PloS one》2013,8(10)
A major challenge for successful immunotherapy against glioma is the identification and characterization of validated targets. We have taken a bioinformatics approach towards understanding the biological context of IL-13 receptor α2 (IL13Rα2) expression in brain tumors, and its functional significance for patient survival. Querying multiple gene expression databases, we show that IL13Rα2 expression increases with glioma malignancy grade, and expression for high-grade tumors is bimodal, with approximately 58% of WHO grade IV gliomas over-expressing this receptor. By several measures, IL13Rα2 expression in patient samples and low-passage primary glioma lines most consistently correlates with the expression of signature genes defining mesenchymal subclass tumors and negatively correlates with proneural signature genes as defined by two studies. Positive associations were also noted with proliferative signature genes, whereas no consistent associations were found with either classical or neural signature genes. Probing the potential functional consequences of this mesenchymal association through IPA analysis suggests that IL13Rα2 expression is associated with activation of proinflammatory and immune pathways characteristic of mesenchymal subclass tumors. In addition, survival analyses indicate that IL13Rα2 over-expression is associated with poor patient prognosis, a single gene correlation ranking IL13Rα2 in the top ~1% of total gene expression probes with regard to survival association with WHO IV gliomas. This study better defines the functional consequences of IL13Rα2 expression by demonstrating association with mesenchymal signature gene expression and poor patient prognosis. It thus highlights the utility of IL13Rα2 as a therapeutic target, and helps define patient populations most likely to respond to immunotherapy in present and future clinical trials. 相似文献
14.
15.
Yihao Yang Ziyan Shen Youguang Li Chenda Xu Han Xia Hao Zhuang Shengyuan Sun Min Guo Changjie Yan 《植物学报(英文版)》2022,64(10):1860-1865
Rice eating and cooking quality (ECQ) is a major concern of breeders and consumers, determining market competitiveness worldwide. Rice grain protein content (GPC) is negatively related to ECQ, making it possible to improve ECQ by manipulating GPC. However, GPC is genetically complex and sensitive to environmental conditions; therefore, little progress has been made in traditional breeding for ECQ. Here, we report that CRISPR/Cas9-mediated knockout of genes encoding the grain storage protein glutelin rapidly produced lines with downregulated GPC and improved ECQ. Our finding provides a new strategy for improving rice ECQ. 相似文献
16.
17.
18.
19.
Consequence of Absence of Nitrate Reductase Activity on Photosynthesis in Nicotiana plumbaginifolia Plants 总被引:6,自引:1,他引:5
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Saux C Lemoine Y Marion-Poll A Valadier MH Deng M Morot-Gaudry JF 《Plant physiology》1987,84(1):67-72
Chlorate-resistant Nicotiana plumbaginifolia (cv Viviani) mutants were found to be deficient in the nitrate reductase apoprotein (NR−nia). Because they could not grow with nitrate as sole nitrogen source, they were cultivated as graftings on wild-type Nicotiana tabacum plants. The grafts of mutant plants were chlorotic compared to the grafts of wild type. Mutant leaves did not accumulate nitrogen and nitrate but contained less malate and more glutamine than wild leaves. They exhibited a slight increase of the proportion of the light-harvesting chlorophyll a/b protein complexes and a lowering of the efficiency of energy transfer between these complexes and the active centers. After a 3 second 14CO2 pulse, the total 14C incorporation of the mutant leaves was approximately 20% of that of the control. The 14C was essentially recovered in ribulose bisphosphate in these plants. It was consistent with a decline of ribulose bisphosphate carboxylase activity observed in the mutant. After a 3 second 14CO2 pulse followed by a 60 second chase with normal CO2, 14C was mainly accumulated in starch which was labeled more in the mutant than in the wild type. These results confirm the observation that in the nitrate reductase deficient leaves, chloroplasts were loaded with large starch inclusions preceding disorganization of the photosynthetic apparatus. 相似文献
20.
Summary The alder has a perennial nodule cluster. The nodule amount on the roots increases with tree age. The N2-fixing activity of nodules decreases with nodule age. Purple coloured soils with various soil pHs and CaCO3 contents are, in the main, the ones which influence nodulation and N2-fixing. Higher N2-fixing capacity existed in the neutral and low calcium soils. High calcium soils and acid soils can restrain nodulation and
the N2-fixing rate significantly. On the slope, where calcarous light loams are found, the annual nitrogen fixation capacity of
alder and cypress mixed plantations, less than 10 years old, is 16 or 17 kg/ha yr, but in the valley, a pure alder plantation
can reach 40 kg/ha yr. 相似文献