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201.
Barbara M. Mullock Miloslav Dobrota Richard H. Hinton 《Biochimica et Biophysica Acta (BBA)/General Subjects》1978,543(4):497-507
The protein composition of rat bile has been studied systematically using two-dimensional agarose-polyacrylamide gel electrophoresis, with or without prior absorption by immobilised antisera, and by crossed immunoelectrophoresis. Sixteen bile proteins were distinguished. Of these, thirteen are immunologically identical to proteins present in rat serum and only one is identical to a protein present in rat liver plasma membrane but not in rat serum. Of the remaining two proteins, one is bile lipoprotein and the other has many of the properties of immunoglobulin A secretory component.The serum-related proteins in rat bile fall into two distinct groups. In the first group are immunoglobulin A and an α2-globulin. These proteins are major constituents of bile but only minor constituents of serum. In the second group are albumin and some other major serum proteins which are found in bile at concentrations less than 1% of their concentrations in serum. The relative proportions of these proteins in bile appear to differ from their proportions in serum. It therefore appears that, although the majority of bile proteins are derived from serum, there cannot be direct leakage of serum into bile. Examination of the proteins contained within liver lysosomes indicates that, although discharge of lysosomal contents at the bile canalicular face of the hepatocyte may contribute to the bile proteins, an additional mechanism, with a considerable degree of selectivity, must also be involved in the transport of proteins from serum to bile. 相似文献
202.
The rate of the maturation process of avian myeloblastosis virus experimentally estimated on the basis of genomic viral RNA conversion and morphological transition of virions was mathematically analysed. Three mathematical models were suggested and fitted to experimental data. It was found that: (a) model of simple kinetics (Model 1) does not agree with experimental data. Therefore, two hypotheses were considered in further mathematical modelling: (b) virions are identical in time of budding: maturation is dependent on the presence of a virion component which is degraded with time (Model 2). This model agrees with experimental data in all stages of the maturation process. (c) Virions are released from cells at different stages of assembly (Model 3). This model differs from experimental data especially in early stages of maturation. The hypothesis used for the construction of Model 2 seems to be the most plausible to explain the maturation process and is in agreement with data of murine leukemia virus maturation which was found to be accomplished by cleavage of p70 precursor protein. 相似文献
203.
Jiří Kamler Miloslav Homolka Radim Cerkal Marta Heroldová Jarmila Krojerová-Prokešová Miroslava Barančeková Jan Dvořák Karel Vejražka 《European Journal of Wildlife Research》2009,55(6):583-588
Large herbivores are the key species for game management in the Central Europe. Analyses of factors affecting the feeding
behaviour of herbivores and consequences of their browsing are therefore highly important both for farmers and for game managers
as the protective measurements should be focused on the most threatened fields. The influence of fallow (Dama dama) and roe deer (Capreolus capreolus) browsing during vegetation period on sunflower production was studied. The experiment was carried out in 2006 on a field
located near a forest complex. We marked out pairs of paired permanent plots and monitored deer impact regularly from the
emergence of plants until the harvest. Herbivores damaged the sunflower intensively immediately after the emergence at the
height of 1 to 2 cm above the ground. When the plants had reached approximately 15-40 cm in height, deer browsed the top shoots
and as the plants grew higher the browsing was restricted to leaves or to substitute stems of the previously browsed plants.
Sunflower ceased to attract big herbivores in flowering stage. Browsing significantly affected the yield of seeds. Approximately
one half of the plants that had been damaged in early stage died as well as 12.5% of plants that had been damaged at second
term; the rest produced substitute stems. Almost 33% of the substitute stems did not form disc florets; the second third of
them formed disc florets with a diameter of 6-8 cm, and the last third set disc florets with a diameter of 8-10 cm. 相似文献
204.