全文获取类型
收费全文 | 186篇 |
免费 | 3篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 7篇 |
2013年 | 6篇 |
2012年 | 7篇 |
2011年 | 17篇 |
2010年 | 6篇 |
2009年 | 13篇 |
2008年 | 11篇 |
2007年 | 14篇 |
2006年 | 13篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1991年 | 6篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1966年 | 2篇 |
1958年 | 2篇 |
1957年 | 5篇 |
1956年 | 3篇 |
排序方式: 共有189条查询结果,搜索用时 15 毫秒
111.
112.
Background
Insects have an efficient self-defense system that is based on innate immunity. Recent findings have disclosed many parallels between human and insect innate immunity, and simultaneously fine differences in the processes between various species have been revealed. Studies on the immune systems of various insect species may uncover the differences in their host defense strategies. 相似文献113.
Alice Ciencialová Tereza Neubauerová Miloslav Sanda Radek Sindelka Josef Cvacka Zdenek Voburka Milos Budesínsky Václav Kasicka Petra Sázelová Veronika Solínová Martina Macková Bohumír Koutek Jirí Jirácek 《Journal of peptide science》2008,14(6):670-682
We chose the larvae of fleshfly Sarcophaga bullata to map the peptide and protein immune response. The hemolymph of the third-instar larvae of S. bullata was used for isolation. The larvae were injected with bacterial suspension to induce an antimicrobial response. The hemolymph was separated into crude fractions, which were subdivided by RP-HPLC, gel electrophoresis, and free-flow electrophoresis. In several fractions, we determined significant antimicrobial activities against the pathogenic bacteria Escherichia coli, Staphylococcus aureus, or Pseudomonas aeruginosa. Among antimicrobially active compounds we identified dipeptide beta-alanyl-L-tyrosine, protein transferrin, and two variants of peptide sapecin. We also partially characterized two novel antimicrobially active polypeptides; odorant-binding protein 99b, and a peptide which remains unidentified. 相似文献
114.
Stanislav Pavelka Arnošt Babický Miloslav Vobecký Jaroslav Lener 《Biological trace element research》2001,82(1-3):133-142
The effect of a high bromide intake on the kinetics of iodide uptake and elimination in the thyroid and skin of adult male
rats was studied. In rats fed a diet with sufficient iodine supply (>25 μg I/d), the iodide accumulation in the skin predominated
during the first hours after 131I -iodide application. From this organ, radioiodide was gradually transferred into the thyroid. A high bromide intake (>150
mg Br−/d) in these animals led to a marked decrease in iodide accumulation, especially by the thyroid, because of an increase in
iodide elimination both from the thyroid and from the skin. In rats kept under the conditions of iodine deficiency (<1 μ I/d),
the iodide accumulation in the thyroid, but not in the skin, was markedly increased as a result of a thyrotropic stimulation.
The effect of a high bromide intake (>100 mg Br−/d) in these animals was particularly pronounced because the rates of iodide elimination were most accelerated both from their
thyroid and from their skin.
Presented in part at the 20th Workshop on Macro and Trace Elements held in Jena (Germany) on December 1–2, 2000. 相似文献
115.
Vladimír Kren Anna Fiserová Lenka Weignerová Ivan Stibor Petr Halada Vera Prikrylová Petr Sedmera Miloslav Pospísil 《Bioorganic & medicinal chemistry》2002,10(2):415-424
Dimers of agroclavine (1) and terguride (2), as well as a series of terguride oligomers, for example trimers (5, 6), tetramer (7), hexamer (8) and functionalized tergurides for further complex clustering were synthesized. Terguride oligomers were screened for their direct cellular toxicity on lymphoma cell lines in vitro and for their immunomodulating activities, represented by the natural killer (NK) cell-mediated cytotoxicity, as the most sensitive screening marker during immune responses. Dimers linked via aromatic spacer showed a high toxicity (1 microM) to lymphoma cells, which was not detected in other derivatives. In vitro and ex vivo experiments performed on mouse spleen lymphocytes in the presence of terguride oligomers demonstrated an immunosuppressive effect of dimers with aromatic spacer (4c-d) and NK cell stimulatory effect of terguride hexamer (8) and trimer with aliphatic spacer (5c). There is a considerable evidence that indolic part of molecule contributes to immunosuppressive action of terguride, which is potentiated in dimers carrying aromatic linker. This effect can be reversed by higher oligomerization of the respective alkaloids. 相似文献
116.
Záková L Kazdová L Hanclová I Protivínská E Sanda M Budesínský M Jirácek J 《Biochemistry》2008,47(21):5858-5868
In this study, we prepared several shortened and full-length insulin analogues with substitutions at position B26. We compared the binding affinities of the analogues for rat adipose membranes with their ability to lower the plasma glucose level in nondiabetic Wistar rats in vivo after subcutaneous administration, and also with their ability to stimulate lipogenesis in vitro. We found that [NMeHisB26]-DTI-NH 2 and [NMeAlaB26]-DTI-NH 2 were very potent insulin analogues with respect to their binding affinities (214 and 465%, respectively, compared to that of human insulin), but they were significantly less potent than human insulin in vivo. Their full-length counterparts, [NMeHisB26]-insulin and [NMeAlaB26]-insulin, were less effective than human insulin with respect to binding affinity (10 and 21%, respectively) and in vivo activity, while [HisB26]-insulin exhibited properties similar to those of human insulin in all of the tests we carried out. The ability of selected analogues to stimulate lipogenesis in adipocytes was correlated with their biological potency in vivo. Taken together, our data suggest that the B26 residue and residues B26-B30 have ambiguous roles in binding affinity and in vivo activity. We hypothesize that our shortened analogues, [NMeHisB26]-DTI-NH 2 and [NMeAlaB26]-DTI-NH 2, have different modes of interaction with the insulin receptor compared with natural insulin and that these different modes of interaction result in a less effective metabolic response of the insulin receptor, despite the high binding potency of these analogues. 相似文献
117.
We describe two new species of Eimeria Schneider, 1875 from the gerbiline rodent Gerbilliscus guineae in the Niokolo Koba National Park, Senegal. Faecal examination of samples revealed the presence of sporulated oöcysts of two eimerian coccidia, both possessing an oöcyst residuum. Eimeria permira n. sp. is remarkable in terms of oöcyst size and oöcyst wall texture. Sporulated oöcysts are ellipsoidal, 45.8 (42–50) × 32.5 (31–38) μm; the oöcyst wall is 3–4 μm thick, composed of three layers, with the outer layer sheathed by rough granular material; and the sporocysts are broadly ellipsoidal, 15.4 (15–16) × 11 and with a Stieda body present. Oöcysts of Eimeria gerbillisci n. sp. are subspherical, 22.5 (19.5–24) × 18.8 (16.5–20) μm, with a colourless, faintly granulated oöcyst wall 1.5 thick; and the sporocysts are 10.1 (10–12) × 6.7 (6–8), broadly ellipsoidal and often somewhat pointed towards both ends. 相似文献
118.
119.
Methanogenesis-driven ATP synthesis in a neomycin-resistant mutant of Methanothermobacter thermautotrophicus (formerly Methanobacterium thermoautotrophicum strain DeltaH) was strongly inhibited at both pH 6.8 and pH 8.5 by the uncoupler 3,3',4',5 -tetrachlorosalicylanilide (TCS) in the presence of either 1 or 10 mM NaCl. The generation of a membrane potential in the mutant cells at pH 6.8 was also strongly inhibited by TCS in the presence of 1 or 10 mM NaCl. On the other hand, at pH 8.5 in the presence of 10mM NaCl, a protonophore-resistant membrane potential of approximately 150 mV was found. These results indicate that in the mutant cells the process of energy transduction between methanogenesis and membrane potential generation is not impaired. In contrast to the wild-type strain, ATP synthesis in the mutant cells was driven by an electrochemical gradient of H(+) under alkaline conditions. Unlike wild-type cells, the mutant lacks the capacity to transduce an uncoupler-resistant membrane potential energy at pH 8.5 into ATP synthesis. Na(+)/H(+) exchange was comparable in the wild type and the mutant cells. Western blots of sub-cellular fractions with polyclonal antiserum reactive to the B-subunit of the halobacterial A-type H(+)-translocating ATPase confirmed the presence of A-type ATP synthase in the mutant cells. Furthermore, in the mutant cells a protein band of molecular mass about 45 kDa is absent but there was an abundant protein band at about 67 kDa. Based on the observed bioenergetic features of the mutant cells, neither the A(1)A(o) ATP synthase alone nor together with the Na(+)/H(+) antiporter seems to be responsible for ATP synthesis driven by sodium motive force. Rather, some other links between neomycin-resistance and failure of sodium motive force-dependent ATP synthesis in the neomycin resistant mutant are discussed. 相似文献
120.
The parallel course of the excretion rates of sodium and bromide ions was demonstrated in adult male rats administered simultaneously
with 24Na-sodium chloride and 82Br-bromide. These excretion rates were inversely proportional to the magnitude of sodium intake in the animals. The biological
half-life of bromide, as a substitute for sodium or chloride, was investigated with the aid of the radionuclide 82Br in animals situated in very different physiological states (i.e., in lactating and nonlactating female rats as well as
in young rats of varying ages [2,4,6, and 10 wk of age]). The 82Br radioactivity retained in mothers and in whole litters was measured in vivo at appropriate time intervals (up to 240 h)
after the application of 82Br-bromide to the mothers. The time-course of the changes in the 82Br radioactivity of the young was calculated as the difference between the rate of 82Br intake in the mother's milk and the 82Br excretion through the kidneys into the urine. The rate of 82Br excretion through the kidneys of the dam could be calculated also. Nonweaned young rats (12 d) had the highest half-life
(269 h) and lactating dams had the lowest (44 h). The determined values demonstrated that nonweaned young apparently conserve
sodium, because of its relatively low concentration in mother's milk, whereas lactating dams, because of their large food
intake, waste sodium.
Presented in part at the 4th International Symposium on Trace Elements in Human: New Perspectives held in Athens (Greece)
on 9–11 October 2003 相似文献