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151.
152.
Maria Francesca Mossuto Sara Sannino Davide Mazza Claudio Fagioli Milena Vitale Edgar Djaha Yoboue Roberto Sitia Tiziana Anelli 《PloS one》2014,9(10)
Precise coordination of protein biogenesis, traffic and homeostasis within the early secretory compartment (ESC) is key for cell physiology. As a consequence, disturbances in these processes underlie many genetic and chronic diseases. Dynamic imaging methods are needed to follow the fate of cargo proteins and their interactions with resident enzymes and folding assistants. Here we applied the Halotag labelling system to study the behavior of proteins with different fates and roles in ESC: a chaperone, an ERAD substrate and an aggregation-prone molecule. Exploiting the Halo property of binding covalently ligands labelled with different fluorochromes, we developed and performed non-radioactive pulse and chase assays to follow sequential waves of proteins in ESC, discriminating between young and old molecules at the single cell level. In this way, we could monitor secretion and degradation of ER proteins in living cells. We can also follow the biogenesis, growth, accumulation and movements of protein aggregates in the ESC. Our data show that protein deposits within ESC grow by sequential apposition of molecules up to a given size, after which novel seeds are detected. The possibility of using ligands with distinct optical and physical properties offers a novel possibility to dynamically follow the fate of proteins in the ESC. 相似文献
153.
The optimum conditions for cell suspension culture experiments have been characterized by respiration,p-Phenylenediamine oxidase and growth of cell suspension ofNicotiana tabacum for 15 days in stationary culture. Change of 3,4-Dihydroxybenzoic and 4-Hydroxybenzoic acids both present at 10−4 M in culture medium were studied in short term experiments. An oxidation product of 3,4-Dihydroxybenzoic acid characterized
by an absorption maximum at 280 nm was isolated after 1 h of incubation. The 4-Hydroxybenzoic acid did not change when incubated
for 4 h under the same conditions. 相似文献
154.
155.
Cássio S. Meira José Maurício dos Santos Filho Caroline C. Sousa Pâmela S. Anjos Jéssica V. Cerqueira Humberto A. Dias Neto Rafael G. da Silveira Helena M. Russo Jean-Luc Wolfender Emerson F. Queiroz Diogo R.M. Moreira Milena B.P. Soares 《Bioorganic & medicinal chemistry》2018,26(8):1971-1985
4-(Nitrophenyl)hydrazone derivatives of N-acylhydrazone were synthesized and screened for suppress lymphocyte proliferation and nitrite inhibition in macrophages. Compared to an unsubstituted N-acylhydrazone, active compounds were identified within initial series when hydroxyl, chloride and nitro substituents were employed. Structure-activity relationship was further developed by varying the position of these substituents as well as attaching structurally-related substituents. Changing substituent position revealed a more promising compound series of anti-inflammatory agents. In contrast, an N-methyl group appended to the 4-(nitrophenyl)hydrazone moiety reduced activity. Anti-inflammatory activity of compounds is achieved by modulating IL-1β secretion and prostaglandin E2 synthesis in macrophages and by inhibiting calcineurin phosphatase activity in lymphocytes. Compound SintMed65 was advanced into an acute model of peritonitis in mice, where it inhibited the neutrophil infiltration after being orally administered. In summary, we demonstrated in great details the structural requirements and the underlying mechanism for anti-inflammatory activity of a new family of hydrazone-N-acylhydrazone, which may represent a valuable medicinal chemistry direction for the anti-inflammatory drug development in general. 相似文献
156.
Anna Corsini Milena Colombo Claudio Gardana Sarah Zecchin Paolo Simonetti Lucia Cavalca 《Annals of microbiology》2018,68(5):295-304
Achromobacter sp. strain N2 was isolated from a pyrite-cinder-contaminated soil and presented plant growth promoting traits (ACC deaminase activity, production of indole-3-acetic and jasmonic acids, siderophores secretion, and phosphate solubilization) and arsenic transformation abilities. Achromobacter sp. strain N2 was resistant to different metals and metalloids, including arsenate (100 mM) and arsenite (5 mM). The strain was resistant to ionic stressors (i.e., arsenate and NaCl), whereas bacterial growth was impaired by osmotic stress. Strain N2 was able to oxidize 1.0 mmol L?1 of arsenite to arsenate in 72 h. This evidence was supported by the retrieval of an arsenite oxidase AioA gene highly homologous to arsenite oxidases of Achromobacter and Alcaligenes species. Rice seeds of Oryza sativa (var. Loto) were bio-primed with ACCD-induced and non-induced cells in order to evaluate the effect of inoculation on rice seedlings growth and arsenic uptake. The bacterization with ACCD-induced cells significantly improved seed germination and seedling heights if compared with the seeds inoculated with non-induced cells and non-primed seeds. Enhanced arsenic uptake was evidenced in the presence of ACCD-induced cells, suggesting a role of ACCD activity on the mitigation of the toxicity of arsenic accumulated by the plant. This kind of responses should be taken into account when proposing PGP strains for improving plant growth in arsenic-rich soils. 相似文献
157.
Julie Rousseau Zhengxing Zhang Xiaozhu Wang Chengcheng Zhang Joseph Lau Etienne Rousseau Milena Čolović Navjit Hundal-Jabal François Bénard Kuo-Shyan Lin 《Bioorganic & medicinal chemistry letters》2018,28(5):899-905
Two novel bifunctional tetrahydroxamate chelators 3 and 4 were synthesized and evaluated for labeling antibodies with 89Zr for positron emission tomography imaging. Compared to previously reported tetrahydroxamate chelators 1 and 2 with an iminodiacetamide backbone, 3 and 4 were based on an extended iminodipropionamide and dipropylenetriamine backbone, respectively. Trastuzumab conjugates of 3 and 4 were efficiently labeled with 89Zr (>95% radiochemical yield). The in vitro plasma stability of 89Zr-4-Trastuzumab and especially 89Zr-3-Trastuzumab was greatly improved over previously reported 89Zr-1-Trastuzumab and 89Zr-2-Trastuzumab, but their demetalation remained higher and faster than 89Zr-deferoxamine (DFO)-Trastuzumab. These observations were confirmed by PET imaging and biodistribution in mice, with significant higher bone uptake for 89Zr-4-Trastuzumab, followed by 89Zr-3-Trastuzumab, and to a lesser extent for 89Zr-DFO-Trastuzumab. Molecular modeling showed that 3 and 4 with an extended backbone could form eight-coordinate Zr-complexes as compared to only seven-coordinate Zr-complexes of 1 and 2. Our data suggest further elongation of linker length between hydroxamate motifs of this class of chelators is needed to reach a better Zr-coordination configuration and improve in vivo stability. 相似文献
158.
Galante Yves M. Merlini Luca Silvetti Tiziana Campia Paola Rossi Bianca Viani Fiorenza Brasca Milena 《Applied microbiology and biotechnology》2018,102(11):4687-4702
Applied Microbiology and Biotechnology - New biomaterials from renewable sources and the development of “functionalized biopolymers” are fields of growing industrial interest. Plant... 相似文献
159.
Derya Ürkmez Marta Ostrowska Milena Roszkowska Magdalena Gawlak Krzysztof Zawierucha Reinhardt Møbjerg Kristensen 《Marine Biology Research》2018,14(1):1-16
In this paper, Megastygarctides sezginii sp. nov., a new marine species from the Black Sea (Turkey), is described. Morphological and morphometric studies have revealed that M. sezginii sp. nov. is most similar to M. setoloso Morgan & O’Reilly, 1988, but differs from it through the presence of two types of fibrils, a lack of cuticle granulation, the presence of three lateral projections with fibrils, leg fibrils arranged in transverse stripes and the presence of clumps of fibrils near the clavae. In the studied environment, the new species is frequently found in summer and almost absent in winter. Additionally, a taxonomic key for all Megastygarctides species is presented.
http://zoobank.org/urn:lsid:zoobank.org:pub:53AA1D57-8700-4A18-9099-1CDDBAEB4D3A 相似文献
160.