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81.
Gonçalves Renata C. Carvalho Celso Carneiro Michels Monique Abatti Mariane R. Manfredini Andressa Silva Milena C. Dominguini Diogo Steckert Amanda Mina Francielle Streck Emílio Budni Josiane Dal-Pizzol Felipe 《Neurochemical research》2022,47(3):613-621
Neurochemical Research - Critical illness encompasses a wide spectrum of life-threatening clinical conditions requiring intensive care. Our objective was to evaluate cognitive, inflammatory and... 相似文献
82.
Milena S. Espíndola Leonardo J. G. Lima Luana S. Soares Maira C. Cacemiro Fabiana A. Zambuzi Matheus de Souza Gomes Laurence R. Amaral Valdes R. Bollela Olindo A. Martins-Filho Fabiani G. Frantz 《PloS one》2015,10(12)
Background
Successful highly active antiretroviral therapy (HAART) has changed the outcome of AIDS patients worldwide because the complete suppression of viremia improves health and prolongs life expectancy of HIV-1+ patients. However, little attention has been given to the immunological profile of patients under distinct HAART regimens. This work aimed to investigate the differences in the immunological pattern of HIV-1+ patients under the first- or second-line HAART in Brazil.Methods
CD4+ T cell counts, Viral load, and plasma concentration of sCD14, sCD163, MCP-1, RANTES, IP-10, IL-1β, IL-6, TNF-α, IL-12, IFN-α, IFN-γ, IL-4, IL-5, and IL-10 were assessed for immunological characterization of the following clinical groups: Non-infected individuals (NI; n = 66), HIV-1+ untreated (HIV; n = 46), HIV-1+ treated with first-line HAART (HAART 1; n = 15); and HIV-1+ treated with second-line HAART (HAART 2; n = 15).Results
We found that the immunological biosignature pattern of HAART 1 is similar to that of NI individuals, especially in patients presenting slow progression of the disease, while patients under HAART 2 remain in a moderate inflammatory state, which is similar to that of untreated HIV patients pattern. Network correlations revealed that differences in IP-10, TNF-α, IL-6, IFN-α, and IL-10 interactions were primordial in HIV disease and treatment. Heat map and decision tree analysis identified that IP-10>TNF-α>IFN-α were the best respective HAART segregation biomarkers.Conclusion
HIV patients in different HAART regimens develop distinct immunological biosignature, introducing a novel perspective into disease outcome and potential new therapies that consider HAART patients as a heterogeneous group. 相似文献83.
V Perić Mataruga M Vlahović B Janać L Ilijin M Janković Tomanić D Matić M Mrdaković 《Peptides》2012,37(1):55-62
Ghrelin is a 28-amino acid peptide that has significant effects on appetite and growth in humans and animals. The aim of this study was to examine 4th instar larvae of the pest insect Lymantria dispar L. after ghrelin treatment. Parameters included changes in nutritional indices (efficiency of conversion of ingested food, efficiency of conversion of digested food, approximate digestibility); midgut and fat body mass; total proteases, trypsin and leucine aminopeptidase activities in the midgut; number, height and width of columnar and goblet cells and their nuclei in the midgut epithelium and detection of ghrelin-like immunoreactivity in the midgut tissue. Four subpicomolar injections of ghrelin (0.3pmol) or physiological saline (control) were applied every 24h. The nutritional indices were higher in the ghrelin treated than in the control group. Ghrelin treatment was also associated with elevation of midgut mass, induced digestive enzyme activities, increased fat body mass and morphometric changes in columnar and goblet cells. This is the first report of the presence of ghrelin-like hormone in endocrine cells of an insect midgut. Such information provides additional evidence for application of this relatively simple model system in the future studies of the mechanisms underlying of digestion and energy balance in more complex organisms. 相似文献
84.
Kobets T Havelková H Grekov I Volkova V Vojtíšková J Slapničková M Kurey I Sohrabi Y Svobodová M Demant P Lipoldová M 《PLoS neglected tropical diseases》2012,6(6):e1667
Background
Leishmaniasis is a disease caused by protozoan parasites of genus Leishmania. The frequent involvement of Leishmania tropica in human leishmaniasis has been recognized only recently. Similarly as L. major, L. tropica causes cutaneous leishmaniasis in humans, but can also visceralize and cause systemic illness. The relationship between the host genotype and disease manifestations is poorly understood because there were no suitable animal models.Methods
We studied susceptibility to L. tropica, using BALB/c-c-STS/A (CcS/Dem) recombinant congenic (RC) strains, which differ greatly in susceptibility to L. major. Mice were infected with L. tropica and skin lesions, cytokine and chemokine levels in serum, and parasite numbers in organs were measured.Principal Findings
Females of BALB/c and several RC strains developed skin lesions. In some strains parasites visceralized and were detected in spleen and liver. Importantly, the strain distribution pattern of symptoms caused by L. tropica was different from that observed after L. major infection. Moreover, sex differently influenced infection with L. tropica and L. major. L. major-infected males exhibited either higher or similar skin pathology as females, whereas L. tropica-infected females were more susceptible than males. The majority of L. tropica-infected strains exhibited increased levels of chemokines CCL2, CCL3 and CCL5. CcS-16 females, which developed the largest lesions, exhibited a unique systemic chemokine reaction, characterized by additional transient early peaks of CCL3 and CCL5, which were not present in CcS-16 males nor in any other strain.Conclusion
Comparison of L. tropica and L. major infections indicates that the strain patterns of response are species-specific, with different sex effects and largely different host susceptibility genes. 相似文献85.
86.
Jimmy?Cabra-GarcíaEmail author Christian?Bermúdez-Rivas Ana?Milena?Osorio Patricia?Chacón 《Biodiversity and Conservation》2012,21(6):1493-1508
In this study α and β diversity patterns of five leaf litter arthropod groups (ants, predatory ants, oribatid mites, spiders
and other arachnids) were described and compared in 39 sampling patches of a transformed landscape in southwestern Colombia,
that represented five vegetation types: secondary forest, riparian forest, giant bamboo forest, pasture and sugarcane crop.
It was also assessed whether some taxa could be used as diversity surrogates. A total of 6,765 individuals grouped in 290
morphospecies were collected. Species richness in all groups was lower in highly transformed vegetation types (pasture, sugarcane
crop) than in native ones (forests). In contrast, there were no clear tendencies of β diversity among vegetation types. Considering
sampling patches, 0.1–42% of the variation in α diversity of one taxonomic group could be explained from the α diversity of
another, and 0.2–33% of the variation of β diversity of a given taxon was explained by that in other groups. Contrary to recent
findings, we concluded that patterns of α diversity are more congruent than patterns of β diversity. This fact could be attributed
to a sampling effect that promotes congruence in α diversity and to a lack of a clear regional ecological gradient that could
promote congruent patterns of β diversity. We did not find evidence for an ideal diversity surrogate although diversity patterns
of predatory ants had the greatest congruencies. These results support earlier multi-taxon evaluations in that conservation
planning should not be based on only one leaf litter arthropod group. 相似文献
87.
Oliveira Jairo P. Prado Adilson R. Keijok Wanderson J. Valotto Rafaela S. Silva André R. Guimarães Marco C. C. 《Plasmonics (Norwell, Mass.)》2019,14(3):743-753
Plasmonics - Nano-surface modification techniques have been increasingly reported in a multitude of applications. However, the metal-ligand interface can be difficult to characterize, mainly due to... 相似文献
88.
89.
Milena Groth-Malonek Jochen Heinrichs Harald Schneider S.R.S. Robbert Gradstein 《Organisms Diversity & Evolution》2004,4(1-2):51-57
Sequences of the ITS1–5.8S–ITS2 region of nuclear ribosomal DNA were generated for 12 species from 9 genera of Lejeuneaceae and a single species of Jubulaceae (outgroup). The taxon sampling of Lejeuneaceae included representatives of the two widely recognized subfamilies, Lejeuneoideae and Ptychanthoideae. The molecular dataset was analysed independently and in combination with a morphological dataset. The nrITS dataset and the combined dataset resulted in identical topologies. The genus Bryopteris, sometimes treated as a separate family Bryopteridaceae, is nested within the Lejeuneaceae subfamily Ptychanthoideae. Lejeuneaceae subfamily Lejeuneoideae proved to be paraphyletic with the tribe Lejeuneeae sister to Ptychanthoideae, albeit without significant bootstrap support. The tribes Brachiolejeuneeae and Cheilolejeuneeae of Lejeuneoideae, established recently based on morphological evidence, are well supported in bootstrap analyses both of the ITS and the combined molecular–morphological datasets. The results support classifications of Lejeuneaceae based on morphological data and demonstrate the usefulness of the ITS region for phylogenetic studies within or among closely related genera of Lejeuneaceae. 相似文献
90.
Guimarães-Gomes V Oliveira-Carvalho AL Junqueira-de-Azevedo IL S Dutra DL Pujol-Luz M Castro HC Ho PL Zingali RB 《Archives of biochemistry and biophysics》2004,432(1):1-11
Lectins are carbohydrate-binding molecules that mediate a variety of biological processes. In this work, we identify and characterize a lectin from Bothrops insularis venom, with respect to its biochemical properties and theoretical structure. Initially, from a venom gland cDNA library, we cloned and sequenced a cDNA encoding a protein with high identity to snake venom lectins. A lectin molecule was purified to homogeneity from the venom by affinity column and gel filtration. This protein named BiL displayed hemagglutinating activity that was inhibited by galactose, lactose, and EDTA. Mass spectrometry analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that BiL is a disulfide-linked dimeric protein consisting of monomers with 16,206 m/z. The amino acid sequence, deduced from its cDNA sequence, was confirmed by Edman sequencing and by peptide mass fingerprint analysis. BiL shows similarity to other C-type lectin family members. Modeling studies provide insights into BiL dimeric structure and its structural determinants for carbohydrate and calcium binding. 相似文献