Integrating Multiple Toxicological Endpoints in a Decision-Making Framework for Contaminated Sediments

Contaminated sediment has been identified as one of the major impediments to ecosystem restoration, but there has been little progress made in the management of sediment contaminants. Four primary lines of evidence are generally required for informed assessments yet the integration of these various lines of evidence is problematic. Using data from 220 reference sites located in the nearshore zone of the Laurentian Great Lakes the normal response of four species of laboratory organisms to sediments representing a wide range of sediment characteristics was examined. The toxicity data from the reference sites were used to establish categories of responses to test sediments. The delineations for the three categories were developed from the standard statistical parameters of population mean and standard deviation (mean ± SD) of an endpoint measured in all reference sediments. Three approaches for integrating information were examined; the first two are score based, the third approach uses a multivariate statistical method to integrate the responses. The methods were examined using both artificial and real test site data and from this it was concluded that ordination is the superior of the three. It is the least subjective within the context of the integration of the endpoints, is quantitative, and also provides appropriate weighting based on the variation observed within reference sites.     

The mud mound nature of the Cassian Platform Margins of the Dolomites A case history: the Cipit boulders from Punta Grohmann (Sasso Piatto Massif, northern Italy)

Summary The sedimentological features and the microbiofacies of the Cassian platforms (Late Ladinian-Carnian) of the Dolomites can be studied only on the basis of the socalled “Cipit boulders”, that are platform-derived olistoliths and clasts fed to the basin and escaped to the extensive dolomitization affecting the buildups. Our paper deals with the Cipit boulders occurring in the Punta Grohmann section (Wengen and S. Cassiano formations, Late Ladinian, Archelaus and Regoledanus Zones). The dominant microfacies are represented by boundstone, consisting of nearly 60% of micritic limestone occurring both as peloidal or aphanitic micrite, mostly organized into stromatolitic laminites of thrombolites. The skeletal organism (Tubiphytes, skeletal cyanobacteria, sphinctozoan sponges, etc.) represent only a minor component of the rock (usually less than 10%). Early cements are widespread and consist both of fan-shaped calcite (replacing former aragonite), bladed isopachous magnesian calcite and radial-fibrous calcite (neomorphic after Mg-calcite). The carbonate platforms from which the olistoliths derive were made up mainly of carbonate mud that underwent early lithification, as witnessed by the considerable amount of early cements: therefore they may be regarded to as mudmounds, and more precisely as microbial mud-mounds, due to the clearly accretionary, organic-controlled nature of most micrites. The micrites, subdivided into auto- and allomicrite on the basis of micromorphological and fabric characteristics, have been tested for epifluorescence. The results confirm the organic control on the deposition of automicrite, also in the cases in which a microbial influence is not obvious (i.e. aphanitic micrite without internal organization).     

Synaptonemal Complex and Recombination Nodules in Wild-Type DROSOPHILA MELANOGASTER Females

Electron microscope serial section reconstruction analysis of all zygotene-pachytene nuclei of meiotic cells from three wild-type germaria (a subunit of the ovary containing the early meiotic stages arrayed in temporal developmental sequence) of Drosophila melanogaster females corroborates and extends earlier observations (Carpenter 1975a) on the nature and sequence of ultrastructural events occurring during the time of meiotic recombination. Emphasis has been placed on (1) the time of appearance and disappearance of the synaptonemal complex (SC) and the changes in its dimensions that accompany a cell's progression through pachytene, and (2) the appearance, disappearance, number and chromosomal locations of recombination nodules (Carpenter 1975b). For both the SC and the recombination nodule the availability of several developmental series has provided an estimate of the biological variability in the properties of these recombination-associated structures. The much more extensive data presented here substantiate the earlier hypothesis that recombination nodules occur at sites where reciprocal meiotic recombination will occur, has occurred, or is occurring. A second morphological type of recombination nodule is reported; it is suggested that the presence of the latter type of nodule may correlate with sites of gene conversion. The hypothesis that there may be two types of meiotic recombination processes is discussed.     

Micromorphological,geochemical, and diagenetic characterization of sirenian ribs preserved in the Late Miocene paleontological site of Cessaniti (southern Calabria,Italy)

The site of Cessaniti (Vibo Valentia, Italy) has been well known since the 19th century for the richness and good preservation of its Miocene fauna and flora. The sedimentary succession of the site represents a paralic system that evolved toward an open-marine environment recording the Tortonian transgression. The fossil assemblage contains rich invertebrate (corals, bivalves, gastropods, brachiopods, echinoids, benthic and planktonic foraminifers) and vertebrate faunas (proboscideans, rhinoceroses, giraffids, bovids, sirenids, marine turtles, and fish remains). The fossils recovered at the Cessaniti site have a relevant role in phylogenetic studies and paleogeographic reconstructions of Late Miocene environments of the southern Italy. This research is focused on the microstructure and preservation state of the fossil bones. Samples of Metaxytherium sp. bones have been analyzed to understand the diagenetic profile of the bone assemblages that characterizes the taphonomic history of the Cessaniti site. The analyses provided a comprehensive account of how bone mineral (bioapatite) has been altered and demonstrated that the post-burial processes did not significantly affect the micromorphological and biogeochemical features of the bones. The excellent preservation state of the bones strengthens the importance of the Cessaniti site for studies of the Mediterranean Miocene vertebrate fauna.     

Teucrium fruticans L., a Multi-Scale Study: From Trichomes to Essential Oil

This work represents the first multi-scale study on Teucrium fruticans L. cultivated at the Ghirardi Botanic Garden (Lombardy, Northern Italy), combining a micromorphological and a phytochemical survey on the plant's aerial parts. Micromorphological investigations, performed by Light Microscopy, Fluorescence Microscopy and Scanning Electron Microscopy, highlighted the presence of five trichomes morphotypes, distinguished by a different distribution pattern: peltates, short-stalked and ball-like medium-stalked capitates, ubiquitous on the whole plant, medium-stalked and long-stalked capitates, exclusive to the floral whorls. Both peltates and medium-stalked capitates were recognized as the main terpene production sites. Phytochemical characterization focused on the essential oils (EOs), obtained by Clevenger-type hydrodistillation in February and April 2022 and characterized by Gas Chromatography-Mass Spectrometry (GC/MS), which resulted mainly formed by sesquiterpene hydrocarbons. The February EO profile was characterized by β-caryophyllene (28.30 %) and germacrene D (19.16 %) as main compounds, while in April β-myrcene was detected at high percentage (13.77 %), in addition to the previous two components (15.72 % and 11.55 %, respectively). Literature data, dealing with the biological activities of the main oil constituents, highlighted an anti-microbial, anti-inflammatory, and anti-tumor potential, due to the high content in sesquiterpenes and, particularly, of β-caryophyllene and germacrene D.