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471.
Andrea?Gramegna Francesco?Amati Leonardo?Terranova Giovanni?Sotgiu Paolo?Tarsia Daniela?Miglietta Maria?Adelaide?Calderazzo Stefano?AlibertiEmail author Francesco?Blasi 《Respiratory research》2017,18(1):211
The role of neutrophil elastase (NE) is poorly understood in bronchiectasis because of the lack of preclinical data and so most of the assumptions made about NE inhibitor potential benefit is based on data from CF. In this context, NE seems to be a predictor of long-term clinical outcomes and a possible target of treatment. In order to better evaluate the role of NE in bronchiectasis, a systematic search of scientific evidence was performed.Two investigators independently performed the search on PubMed and included studies published up to May 15, 2017 according to predefined criteria. A final pool of 31 studies was included in the systematic review, with a total of 2679 patients. For each paper data of interest were extracted and reported in table.In this review sputum NE has proved useful as an inflammatory marker both in stable state bronchiectasis and during exacerbations and local or systemic antibiotic treatment. NE has also been associated with risk of exacerbation, time to next exacerbation and all-cause mortality. This study reviews also the role of NE as a specific target of treatment in bronchiectasis. Inhibition of NE is at a very early stage and future interventional studies should evaluate safety and efficacy for new molecules and formulations. 相似文献
472.
Adriano Guido Antonietta Rosso Rossana Sanfilippo Franco Russo Adelaide Mastandrea 《Geomicrobiology journal》2017,34(10):864-872
Biotic crusts occurring in the Early Pleistocene Rumena Cave, in NW Sicily, have been analyzed from a geomicrobiological point of view. The crusts consist largely of scleractinians and of subordinate bryozoans and serpuloideans, all typical of submarine cave biota. Encrustations document a blind cave in a shadowed setting, or possibly below the fair weather swell zone. Autochthonous and, subordinately, detrital fractions were observed within the skeletal framework of biotic crusts. The syndepositional lithified fraction occurs mainly as very fine-grained laminations. Clotted peloidal and aphanitic (structureless) textures occur in the micrites as well. Autochthonous micrite is always associated with a significant amount of organic matter remains. In caves from the Plemmirio area in SE Sicily, the autochthonous microbial micrite, occurring in the bioconstructions, contains bacterial lipid biomarkers, including abundant compounds derived from sulfate-reducing bacteria. It is likely that a similar microbial mediation was involved in the formation of the autochthonous micrite present in the biotic crusts of the Rumena Cave. 相似文献
473.
Tumor-derived heat shock protein 70 peptide complexes are cross-presented by human dendritic cells 总被引:13,自引:0,他引:13
Noessner E Gastpar R Milani V Brandl A Hutzler PJ Kuppner MC Roos M Kremmer E Asea A Calderwood SK Issels RD 《Journal of immunology (Baltimore, Md. : 1950)》2002,169(10):5424-5432
Our study demonstrates that tumor-derived heat shock protein (HSP)70 chaperones a tyrosinase peptide and mediates its transfer to human immature dendritic cells (DCs) by receptor-dependent uptake. Human tumor-derived HSP70 peptide complexes (HSP70-PC) thus have the immunogenic potential to instruct DCs to cross-present endogenously expressed, nonmutated, and tumor antigenic peptides that are shared among tumors of the melanocytic lineage for T cell recognition. T cell stimulation by HSP70-instructed DCs is dependent on the Ag bound to HSP70 in that only DCs incubated with HSP70-PC purified from tyrosinase-positive (HSP70-PC/tyr(+)) but not from tyrosinase-negative (HSP70-PC/tyr(-)) melanoma cells resulted in the specific activation of the HLA-A*0201-restricted tyrosinase peptide-specific cytotoxic T cell clone. HSP70-PC-mediated T cell stimulation is very efficient, delivering the tyrosinase peptide at concentrations as low as 30 ng/ml of HSP70-PC for T cell recognition. Receptor-dependent binding of HSP70-PC and active cell metabolism are prerequisites for MHC class I-restricted cross-presentation and T cell stimulation. T cell stimulation does not require external DC maturation signals (e.g., exogenously added TNF-alpha), suggesting that signaling DC maturation is an intrinsic property of the HSP70-PC itself and related to receptor-mediated binding. The cross-presentation of a shared human tumor Ag together with the exquisite efficacy are important new aspects for HSP70-based immunotherapy in clinical anti-cancer vaccination strategies, and suggest a potential extension of HSP70-based vaccination protocols from a patient-individual treatment modality to its use in an allogeneic setting. 相似文献
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Cytotechnology - Physical methods are widely utilized to deliver nucleic acids into cells such as electro-transfection or heat shock. An efficient gene electro-transfection requires the best... 相似文献
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Marco Ventura Christian Milani Francesca Turroni Douwe van Sinderen 《Microbial biotechnology》2021,14(1):12-17
The human gut microbiota is involved in multiple health-influencing host interactions during the host’s entire life span. Microbes colonize the infant gut instantaneously after birth and subsequently the founding and interactive progress of this early gut microbiota is considered to be driven and modulated by different host- and microbe-associated forces. A rising number of studies propose that the composition of the human gut microbiota in the early stages of life impact on the human health conditions at later stages of life. This notion has powered research aimed at detailed investigations of the infant gut microbiota composition. Nevertheless, the molecular mechanisms supporting the gut microbiome functionality and the interaction of the early gut microbes with the human host remain largely unknown. 相似文献
480.
Ana I. Gómez-Varela Ricardo Gaspar Adelaide Miranda Juliane L. Assis Rafael H.F. Valverde Marcelo Einicker-Lamas Bruno F. B. Silva Pieter A.A. De Beule 《Journal of biophotonics》2021,14(1):e202000200
The development of nonviral gene delivery vehicles for therapeutic applications requires methods capable of quantifying the association between the genes and their carrier counterparts. Here we investigate the potential of fluorescence cross-correlation spectroscopy (FCCS) to characterize and optimize the assembly of nonviral cationic liposome (CL)-DNA complexes based on a CL formulation consisting of the cationic lipid DOTAP and zwitterionic lipid DOPC. We use a DNA plasmid for lipoplex loading encoding the Oct4 gene, critically involved in reprogramming somatic cells into induced pluripotent stem cells. We demonstrate that FCCS is able to quantitatively determine the extent of the association between DNA and the liposomes and assess its loading capacity. We also establish that the cationic lipid fraction, being proportional to the liposome membrane charge density, as well as charge ratio between the CLs and anionic DNA play an important role in the degree of interaction between the liposomes and DNA. 相似文献