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231.
David Requena Ana Chumbe Michael Torres Ofelia Alzamora Manuel Ramirez Hugo Valdivia-Olarte Andres Hazaet Gutierrez Ray Izquierdo-Lara Luis Enrique Saravia Milagros Zavaleta Luis Tataje-Lavanda Ivan Best Manolo Fernández-Sánchez Eliana Icochea Mirko Zimic Manolo Fernández-Díaz 《Bioinformation》2013,9(10):528-536
Background: Avibacterium paragallinarum, the causative agent of infectious coryza, is a highly
contagious respiratory acute disease of poultry, which affects commercial chickens, laying hens and broilers worldwide.
Methodology: In this study, we performed the whole genome sequencing, assembly and annotation of a Peruvian
isolate of A. paragallinarum. Genome was sequenced in a 454 GS FLX Titanium system. De novo assembly was
performed and annotation was completed with GS De Novo Assembler 2.6 using the H. influenzae str. F3031 gene model.
Manual curation of the genome was performed with Artemis. Putative function of genes was predicted with Blast2GO.
Virulence factors were identified by comparison with the Virulence Factor Database.
Results: The genome obtained has a length of 2.47 Mb with 40.66% of GC content. Seventy five large contigs
(>500 nt) were obtained, which comprised 1,204 predicted genes. All the contigs are available in Genbank
[GenBank: PRJNA64665]. A total of 103 virulence factors, reported in the Virulence Factor Database, were
found in A. paragallinarum. Forty four of them are present in 7 species of Haemophilus, which are related
with pathogenesis, virulence and host immune system evasion. A tetracycline-resistance associated transposon
(Tn10), was found in A. paragallinarum, possibly acting as a defense mechanism.
Discussion and conclusion: The availability of A. paragallinarum genome represents an important source of information for
the development of diagnostic tests, genotyping, and novel antigens for potential vaccines against infectious coryza.
Identification of virulence factors contributes to better understanding the pathogenesis, and planning efforts for prevention
and control of the disease. 相似文献
232.
Kristine M. Molina Mayra L. Estrella Noemi Rivera‐Olmedo Christine Frisard Stephenie Lemon Milagros C. Rosal 《Obesity (Silver Spring, Md.)》2018,26(9):1474-1480
Objective
Evidence suggests discrimination increases the risk of obesity. The biopsychosocial model of racism posits that psychological factors such as depressive symptoms may link experiences of perceived interpersonal discrimination to obesity. This study tested whether self‐reported experiences of everyday discrimination were associated with adiposity indicators and whether depressive symptoms explained these associations.Methods
Cross‐sectional survey data of 602 Latino adults living in Lawrence, Massachusetts, from the Latino Health and Well‐being Project (2011‐2013) were used. Participants completed questionnaires assessing perceived everyday discrimination and depressive symptoms. Anthropometric measures (i.e., BMI and waist circumference [WC]) were obtained by trained staff. Structural equation modeling was employed to test for direct and indirect effects of perceived everyday discrimination on adiposity.Results
Perceived everyday discrimination was directly and positively associated with higher BMI and WC, independent of sociodemographic factors, physical activity, and stressful life events. Perceived everyday discrimination was not indirectly associated with BMI and WC through depressive symptoms. However, perceived everyday discrimination was associated with higher levels of depressive symptoms.Conclusions
Self‐reported everyday discrimination among Latino adults is associated with adiposity. Day‐to‐day interpersonal discrimination may be implicated in obesity disparities for Latino adults.233.
Flavodoxins (Flds) are electron transfer proteins that carry a noncovalently bound flavin mononucleotide molecule (FMN) as a redox active center. A distinguishing feature of these flavoproteins is the dramatic change in the E(sq/rd) reduction potential of the FMN upon binding to the apoprotein (at pH 8.0, from -269 mV when free in solution to -438 mV in Anabaena Fld). In this study, the contribution of three neighboring FMN residues, Thr56, Asn58, and Asn97, and of three negatively charged surface residues, Glu20, Asp65, and Asp96, to modulate the redox properties of FMN upon its binding to the apoprotein has been investigated. Additionally, the role of these residues in the apoflavodoxin:FMN interaction has been analyzed. Concerning the redox potentials, the most noticeable result was obtained for the Thr56Gly mutant. In this Fld variant, the increased accessibility of FMN leads to an increase of +63 mV in the E(sq/rd) value. On the other hand, a correlation between the electrostatic environment of FMN and the E(sq/rd) has been observed. The more positive residues or the less negative residues present in the surroundings of the FMN N(1) atom, then the less negative the value for E(sq/rd). With regard to FMN binding to apoflavodoxin, breaking of hydrophobic interactions between FMN and residues 56, 58, and 97 seems to increase the K(d) values, especially in the Thr56Gly Fld. Such results suggest that the H-bond network in the FMN environment influences the FMN affinity. 相似文献
234.
Nadezhda E. Yun Alexey V. Seregin David H. Walker Vsevolod L. Popov Aida G. Walker Jeanon N. Smith Milagros Miller Juan C. de la Torre Jennifer K. Smith Viktoriya Borisevich Joseph N. Fair Nadia Wauquier Donald S. Grant Bayon Bockarie Dennis Bente Slobodan Paessler 《Journal of virology》2013,87(19):10908-10911
Lassa fever (LF) is a potentially lethal human disease that is caused by the arenavirus Lassa virus (LASV). Annually, around 300,000 infections with up to 10,000 deaths occur in regions of Lassa fever endemicity in West Africa. Here we demonstrate that mice lacking a functional STAT1 pathway are highly susceptible to infection with LASV and develop lethal disease with pathology similar to that reported in humans. 相似文献
235.
Alberto Canfrán-Duque Oscar Pastor Manuel Reina Milagros Lerma Alfonso J. Cruz-Jentoft Miguel A. Lasunción Rebeca Busto 《PloS one》2015,10(10)
Scope
First- and second-generation antipsychotics (FGAs and SGAs, respectively), both inhibit cholesterol biosynthesis and impair the intracellular cholesterol trafficking, leading to lipid accumulation in the late endosome/lysosome compartment. In this study we examined if curcumin, a plant polyphenol that stimulates exosome release, can alleviate antipsychotic-induced intracellular lipid accumulation.Methods
HepG2 hepatocarcinoma cells were treated with antipsychotics or placebo and DiI-labelled LDL for 18 h and then exposed to curcumin for the last 2 h. Cells and media were collected separately and used for biochemical analyses, electron microscopy and immunocytochemistry. Exosomes were isolated from the incubation medium by ultracentrifugation.Results
Curcumin treatment reduced the number of heterolysosomes and shifted their subcellular localization to the periphery, as revealed by electron microscopy, and stimulated the release of lysosomal β-hexosaminidase and exosome markers flotillin-2 and CD63 into the media. The presence of DiI in exosomes released by cells preloaded with DiI-LDL demonstrated the endolysosomal origin of the microvesicles. Furthermore, curcumin increased the secretion of cholesterol as well as LDL-derived DiI and [3H]-cholesterol, in association with a decrease of intracellular lipids. Thus, the disruption of lipid trafficking induced by FGAs or SGAs can be relieved by curcumin treatment. This polyphenol, however, did not mitigate the reduction of cholesterol esterification induced by antipsychotics.Conclusion
Curcumin stimulates exosome release to remove cholesterol (and presumably other lipids) accumulated within the endolysosomal compartment, thereby normalizing intracellular lipid homeostasis. This action may help minimize the adverse metabolic effects of antipsychotic treatment, which should now be evaluated in clinical trials. 相似文献236.
Takaaki Koma Cheng Huang Judith F. Aronson Aida G. Walker Milagros Miller Jeanon N. Smith Michael Patterson Slobodan Paessler 《PLoS neglected tropical diseases》2016,10(8)
Machupo virus (MACV), a New World arenavirus, is the etiological agent of Bolivian hemorrhagic fever (BHF). Junin virus (JUNV), a close relative, causes Argentine hemorrhagic fever (AHF). Previously, we reported that a recombinant, chimeric MACV (rMACV/Cd#1-GPC) expressing glycoprotein from the Candid#1 (Cd#1) vaccine strain of JUNV is completely attenuated in a murine model and protects animals from lethal challenge with MACV. A rMACV with a single F438I substitution in the transmembrane domain (TMD) of GPC, which is equivalent to the F427I attenuating mutation in Cd#1 GPC, was attenuated in a murine model but genetically unstable. In addition, the TMD mutation alone was not sufficient to fully attenuate JUNV, indicating that other domains of the GPC may also contribute to the attenuation. To investigate the requirement of different domains of Cd#1 GPC for successful attenuation of MACV, we rescued several rMACVs expressing the ectodomain of GPC from Cd#1 either alone (MCg1), along with the TMD F438I substitution (MCg2), or with the TMD of Cd#1 (MCg3). All rMACVs exhibited similar growth curves in cultured cells. In mice, the MCg1 displayed significant reduction in lethality as compared with rMACV. The MCg1 was detected in brains and spleens of MCg1-infected mice and the infection was associated with tissue inflammation. On the other hand, all animals survived MCg2 and MCg3 infection without detectable levels of virus in various organs while producing neutralizing antibody against Cd#1. Overall our data suggest the indispensable role of each GPC domain in the full attenuation and immunogenicity of rMACV/Cd#1 GPC. 相似文献
237.
Agustina Olivera-Couto Valentina Salzman Milagros Mailhos Michelle?A. Digman Enrico Gratton Pablo?S. Aguilar 《Biophysical journal》2015,108(7):1633-1644
Eisosomes are plasma membrane domains concentrating lipids, transporters, and signaling molecules. In the budding yeast Saccharomyces cerevisiae, these domains are structured by scaffolds composed mainly by two cytoplasmic proteins Pil1 and Lsp1. Eisosomes are immobile domains, have relatively uniform size, and encompass thousands of units of the core proteins Pil1 and Lsp1. In this work we used fluorescence fluctuation analytical methods to determine the dynamics of eisosome core proteins at different subcellular locations. Using a combination of scanning techniques with autocorrelation analysis, we show that Pil1 and Lsp1 cytoplasmic pools freely diffuse whereas an eisosome-associated fraction of these proteins exhibits slow dynamics that fit with a binding-unbinding equilibrium. Number and brightness analysis shows that the eisosome-associated fraction is oligomeric, while cytoplasmic pools have lower aggregation states. Fluorescence lifetime imaging results indicate that Pil1 and Lsp1 directly interact in the cytoplasm and within the eisosomes. These results support a model where Pil1-Lsp1 heterodimers are the minimal eisosomes building blocks. Moreover, individual-eisosome fluorescence fluctuation analysis shows that eisosomes in the same cell are not equal domains: while roughly half of them are mostly static, the other half is actively exchanging core protein subunits. 相似文献
238.
Apolipoprotein D is the major protein component in cyst fluid from women with human breast gross cystic disease. 总被引:3,自引:0,他引:3
GCDFP(gross-cystic-disease-fluid protein)-24, a progesterone-binding protein present in large amounts in cyst fluid from human breast gross cystic disease, was purified in a one-step procedure by size-exclusion h.p.l.c. Peptide fragments obtained by trypsin digestion of the intact protein were purified by reverse-phase h.p.l.c. and analysed for their amino acid composition and subjected to automated Edman degradation. A search of the National Biomedical Research Foundation Data Bank revealed that all the sequenced tryptic peptides from protein GCDFP-24 matched perfectly with regions present in the amino acid sequence determined for human apolipoprotein D. Additional data on N-terminal sequence of the unblocked proteins, carbohydrate-attachment sites, amino acid composition and molecular-mass estimations supported the identity between both molecules. On the basis of this identity a possible role of apolipoprotein D in progesterone transport is proposed. 相似文献
239.
Milagros R. Mananggit Daria L. Manalo Nobuo Saito Kazunori Kimitsuki Alyssa Marie G. Garcia Patricia Mae T. Lacanilao Joely T. Ongtangco Cornhlo R. Velasco Maria Victoria A. del Rosario Maria Glofezita O. Lagayan Kentaro Yamada Chun-Ho Park Satoshi Inoue Motoi Suzuki Mariko Saito-Obata Yasuhiko Kamiya Catalino S. Demetria Beatriz P. Quiambao Akira Nishizono 《PLoS neglected tropical diseases》2021,15(12)
The direct fluorescent antibody test (dFAT) using brain sample after opening the skull is the standard rabies diagnostic test in animal rabies. However, it is not feasible in many resource-limited settings. Lateral flow devices (LFD) combined with a simple sampling methodology is quicker, simpler, and less hazardous than the standard test and can be a useful tool. We conducted a prospective on-site study to evaluate the diagnostic accuracy of the LFD with the straw sampling method compared with that of the dFAT with the skull opening procedure for post-mortem canine rabies diagnosis. We collected 97 rabies-suspected animals between December 1, 2020 and March 31, 2021. Among the 97 samples, 53 and 50 cases were positive tests for dFAT and LFD, respectively. The sensitivity and specificity of LFD with straw sampling method were 94.3% (95% confidence interval [CI], 84.3–98.8%) and 100% (95% CI, 92.0–100%), respectively. The performance of LFD by the straw sampling method showed relatively high sensitivity and 100% specificity compared with that of dFAT performed on samples collected after opening the skull. This methodology can be beneficial and is a strong tool to overcome limited animal surveillance in remote areas. However, because of our limited sample size, more data using fresh samples on-site and the optimizations are urgently needed for the further implementation in endemic areas. 相似文献
240.
Milagros Medina Ricardo O. Louro Jean Gagnon Maria Luisa Peleato Joaquim Mendes Carlos Gómez-Moreno António V. Xavier M. Teixeira 《Journal of biological inorganic chemistry》1997,2(2):225-234
A soluble monoheme c–type cytochrome c
6 has been isolated from the cyanobacterium Anabaena PCC 7119. It is a basic protein, with a molecular mass of 9.7 kDa, which accepts electrons from Anabaena ferredoxin in the ferredoxin-NADP+reductase-dependent NADPH cytochrome c reductase activity assay. The turnover of the reaction has an optimum pH at 7.5. Flavodoxin can also replace ferredoxin in
this assay, but with only 20% efficiency. Plastocyanin from Anabaena PCC 7119, as well as the c
6 cytochromes from the green algae Chlorella fusca and Monoraphidium braunii are also shown to accept electrons from Anabaena ferredoxin. The reduction potential of cytochrome c
6 at pH 6.7 was determined to be 338 mV and is pH dependent, with pK
a
ox=8.4±0.1 and pK
a
red≈9.5. The ferric and ferrous cytochrome forms and their pH equilibria have been studied using visible, EPR and 1H-NMR spectroscopies. The amino acid sequence and the visible and NMR spectroscopic data indicate that the heme iron has a
methionine-histidine axial coordination in the pH range 5–11. However, the EPR data for the ferricytochrome are complex and
show that in this pH range five distinct forms are present. Between pH 5 and 9 the spectrum is dominated by two rhombic species,
with g–values at 2.94, 2.29, 1.43 and at 2.84, 2.34, 1.56, which interconvert with a pK
a of 8.4. The NMR data also show a main interconversion between two cytochrome forms at this pH, which coincides with that
determined from the pH dependence of the reduction potential. Both these forms were associated with a methionine-histidine
heme-iron coordination by correlation with the visible and NMR spectral data, although having crystal field parameters atypical
for this type of coordination. Anabaena cytochrome c
6 is one more example of a heme protein for which the widely used crystal field analysis of the EPR data (truth diagram) fails
to unequivocally determine the type of heme-iron ligation.
Received: 17 May 1996 / Accepted: 13 January 1997 相似文献