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243.
Melinda Erzsebet Toth Szilvia Gonda Laszlo Vigh Miklos Santha 《Cell stress & chaperones》2010,15(6):807-817
Alcohol induces degeneration of neurons and inhibits neurogenesis in the brain. Small heat shock proteins are able to protect
neurons in cerebral ischemia and oxidative stress. In this study, we investigated the neuroprotective effect of small heat
shock protein, Hsp27, after acute and chronic ethanol administrations using transgenic mice overexpressing the human Hsp27
protein. Transgenic mice and wild-type littermates were injected with 2 g/kg ethanol intraperitoneally, and then motor coordination
and muscle strength were analyzed using different behavioral tests, such as footprint analysis, balance beam, and inverted
screen tests. Ethanol-injected transgenic mice showed similar footprints to control saline-injected mice, did not fall of
the beam, and were able to climb to the top of the inverted screen, while wild-type mice showed ataxia and incoordination
after ethanol injection. The effect of Hsp27 on chronic ethanol consumption was also investigated. Drinking water of mice
was replaced by a 20% ethanol solution for 5 weeks, and then brain sections were stained with Fluoro Jade C staining. We found
significantly lesser amount of degenerating neurons in the brain of ethanol-drinking transgenic mice compared to wild-type
mice. We conclude that Hsp27 can protect neurons against the acute and chronic toxic effects of ethanol. 相似文献
244.
D. Miklos Robkis Eileen M. Hoang Pengse Po Carol J. Deutsch E. James Petersson 《Biopolymers》2021,112(1)
Thioamides, single atom oxygen‐to‐sulfur substitutions of canonical amide bonds, can be valuable probes for protein folding and protease studies. Here, we investigate the fluorescence quenching properties of thioamides incorporated into the side‐chains of amino acids. We synthesize and incorporate Fmoc‐protected, solid‐phase peptide synthesis building blocks for introducing Nε‐thioacetyl‐lysine and γ‐thioasparagine. Using rigid model peptides, we demonstrate the distance‐dependent fluorescence quenching of these thioamides. Furthermore, we describe attempts to incorporate of Nε‐thioacetyl‐lysine into proteins expressed in Escherichia coli using amber codon suppression. 相似文献
245.
The C-terminal cyanogen bromide fragment of VIP, Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2 (all l), was synthesized to provide evidence for the correctness of the sequence proposed by Mutt and Said (1). The synthesis of this hendecapeptide (VIP18–28) was carried out by coupling Z-Ala-Val-(Z)Lys to (Z)Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2. After removal of the protecting groups and purification, the synthetic material was indistinguishable from the natural fragment on paper chromatograms and electropherograms. Their identity was further confirmed by comparison of the products formed on enzymatic hydrolysis. 相似文献
246.
We have isolated and sequenced a cDNA from the shaking-B locus of Drosophila melanogaster. The cDNA contains an open reading frame with extensive homology to another D. melanogaster gene, l(1)ogre. This suggests the existence of a new family of proteins required for the development and maintenance of the D. melanogaster nervous system. 相似文献
247.
Localization and quantification of circular DNA in yeast 总被引:33,自引:0,他引:33
248.
George L. Gabor Miklos Hugh D. Campbell 《Current opinion in genetics & development》1992,2(6):902-906
There is an increasingly heated debate on the very existence of a 'universe of exons' and on the types of genomes that existed after the RNA world. What has been lost in the excitement are the biological issues that relate to the rapid emergence of phenotypic novelties. These issues can be examined by integrating data on protein domains and genomic evolution with the geochemical and palaeontological records. 相似文献
249.
Summary Hybrids were constructed between auxotrophic mutants of a heterothallic distiller's strain and a homothallic wine yeast. The hybridization resulted in a significant increase in both ethanol production and tolerance against exogenous ethanol. The hybrids were heterogeneous in ploidy, probably due to segregation of aneuploids during culturing. Sporulation of the hybrids broke down the high productivity, producing spore clones that were mostly of various intermediate levels of performance. However, a meiotic product superior to both crossing partners was also found. The results demonstrate that fermentation capacity can be improved by crossing with a low performance strain.
Offprint requests to: M. Sipiczki 相似文献