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The C-terminal cyanogen bromide fragment of VIP, Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2 (all l), was synthesized to provide evidence for the correctness of the sequence proposed by Mutt and Said (1). The synthesis of this hendecapeptide (VIP18–28) was carried out by coupling Z-Ala-Val-(Z)Lys to (Z)Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2. After removal of the protecting groups and purification, the synthetic material was indistinguishable from the natural fragment on paper chromatograms and electropherograms. Their identity was further confirmed by comparison of the products formed on enzymatic hydrolysis. 相似文献
374.
We have isolated and sequenced a cDNA from the shaking-B locus of Drosophila melanogaster. The cDNA contains an open reading frame with extensive homology to another D. melanogaster gene, l(1)ogre. This suggests the existence of a new family of proteins required for the development and maintenance of the D. melanogaster nervous system. 相似文献
375.
Localization and quantification of circular DNA in yeast 总被引:33,自引:0,他引:33
376.
George L. Gabor Miklos Hugh D. Campbell 《Current opinion in genetics & development》1992,2(6):902-906
There is an increasingly heated debate on the very existence of a 'universe of exons' and on the types of genomes that existed after the RNA world. What has been lost in the excitement are the biological issues that relate to the rapid emergence of phenotypic novelties. These issues can be examined by integrating data on protein domains and genomic evolution with the geochemical and palaeontological records. 相似文献
377.
Summary Hybrids were constructed between auxotrophic mutants of a heterothallic distiller's strain and a homothallic wine yeast. The hybridization resulted in a significant increase in both ethanol production and tolerance against exogenous ethanol. The hybrids were heterogeneous in ploidy, probably due to segregation of aneuploids during culturing. Sporulation of the hybrids broke down the high productivity, producing spore clones that were mostly of various intermediate levels of performance. However, a meiotic product superior to both crossing partners was also found. The results demonstrate that fermentation capacity can be improved by crossing with a low performance strain.
Offprint requests to: M. Sipiczki 相似文献