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排序方式: 共有1010条查询结果,搜索用时 46 毫秒
41.
Yasuda Tetsuya Mishiro Koji Kusunoki Mikio Fujiwara-Tsujii Nao Yasui Hiroe Uechi Nami Fujimura Takako Inokuchi Rika Fujita Kiwamu Kanegae Yasutada Miura Yasushi Kato Ichiro Mitsunaga Takayuki 《Applied Entomology and Zoology》2020,55(2):205-212
Applied Entomology and Zoology - To evaluate the infection risk of Anoplophora malasiaca (Thomson) (Coleoptera: Cerambycidae) in two species of Japanese pine bonsais (Japanese black pine and... 相似文献
42.
Yuji Nakamura Teppei Fujimoto Yasuyuki Ogawa Hidenori Namiki Sayaka Suzuki Masayoshi Asano Chie Sugita Akiyoshi Mochizuki Shojiro Miyazaki Kazuhiko Tamaki Yoko Nagai Shin-ichi Inoue Takahiro Nagayama Mikio Kato Katsuyoshi Chiba Kiyoshi Takasuna Takahide Nishi 《Bioorganic & medicinal chemistry》2013,21(11):3175-3196
With the aim to address an undesired cardiac issue observed with our related compound in the recently disclosed novel series of renin inhibitors, further chemical modifications of this series were performed. Extensive structure–activity relationships studies as well as in vivo cardiac studies using the electrophysiology rat model led to the discovery of clinical candidate trans-adamantan-1-ol analogue 56 (DS-8108b) as a potent renin inhibitor with reduced potential cardiac risk. Oral administration of single doses of 3 and 10 mg/kg of 56 in cynomolgus monkeys pre-treated with furosemide led to significant reduction of mean arterial blood pressure for more than 12 h. 相似文献
43.
Izumi Yajima Mikio Nakamura Hidemasa Sakakibara Junichi Ide Tetsuya Yanai Kazuo Hayashi 《Bioscience, biotechnology, and biochemistry》2013,77(8):1755-1760
The aqueous extract of dried bonito (Katsuobushi) was distilled under reduced pressure. The resulting distillate with diethyl ether and the extract was separated into acidic, phenolic, basic and neutral fractions. The neutral fraction was further fractionated into ten sub-fractions by silica gel column chromatography. All these sub-fractions were analyzed by gas chromatography and gas chromatography-mass spectrometry.One hundred and sixty-five compounds were identified and 12 compounds were tentatively identified from the neutral fraction. Among them, 111 compounds were newly identified as flavor components of Katsuobushi. 相似文献
44.
Mikio Sato Motoko Suzuki Hajime Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(10):1270-1274
Susceptibility of germinating spores of Bacillus subtilis to a rapid chilling was examined by viable countings. Dormant spores were quite resistant to the cold shock but the spores, immediately upon germination, lose viability almost completely by the same treatment. The presence of divalent cation, magnesium, calcium or manganese, in a buffer to which the germinating spores were suspended, markedly protected the cells from the death by the cold shock effect. When the shocked cells were incubated in the buffer containing casein acid hydrolyzate, glucose and magnesium ion for short period of time, a remarkable increase in viable counts was observed. The existence of two critical temperature zones, which were determined by the initial temperature of cell suspension, was confirmed in the cold shock of germinating spores. 相似文献
45.
Jun-ichi Shimizu Mikio Uehara Masazumi Watanabe 《Bioscience, biotechnology, and biochemistry》2013,77(5):1339-1344
Experiments were done to investigate the volatile components in botrytized grape must and transformation of terpenoids in terpene-supplemented grape must by Botrytis cinerea. Twenty-eight compounds were identified in the volatile concentrate of botrytized must with a combined gas chromatograph-mass spectrometer. No terpenoids were detected in the concentrate. Linalool or terpinen-4-ol decreased a lot when Botrytis cinerea was cultured in the must with these terpenes for 15 days. In linalool-supplemented botrytized must 9 identified and 3 unidentified terpenes were found, while only geranial was detected in terpinen-4-ol-supplemented botrytized must. Botrytis cinerea did not produce terpenoid in grape must without terpenes, but transformed linalool added to grape must into some other monoterpenes. 相似文献
46.
Tetsuko Takabe Mikio Nishimtjra Takashi Akazawa 《Bioscience, biotechnology, and biochemistry》2013,77(10):2137-2142
The isolation of the photosynthetically competent chloroplast preparations was undertaken by means of the density gradient centrifugation on the modified silica sol “Percoll.” A clear separation of the intact chloroplast sustaining the high photosynthetic activities (light dependent CO2 fixation ca. 130μmol/mg Chl·hr) was established. The contamination of mitochondria and peroxisomes was estimated to be less than 3% by measuring the activities of their marker enzymes. The chloroplasts were proved to be free from endoplasmic reticulum and cytosol. The photosynthetic CO2 fixation of the isolated chloroplast preparations was saturated by illumination of the light intensity of 20,000 Lux (12 mW/cm2, 400~750 nm). 相似文献
47.
Takayoshi Higuchi Mikio Shimada Hideo Ōhashi 《Bioscience, biotechnology, and biochemistry》2013,77(12):1459-1465
Characterization of S-adenosylmethionine: catechol O-methyltransferase (EC. 2. 1. 1.6) isolated from bamboo shoot was carried out. Ferulic and sinapic acids which are believed to be lignin precursors are formed by the mediation of the enzyme, and the enzyme activity increased progressively during the lignification of bamboo shoots. Evidences suggest that this enzyme may contribute to the synthesis of lignin precursors. 相似文献
48.
Although the electrolytically obtained DPNH was not completely oxidized by usual dehydrogenases or diaphorases, one of the authors noticed that its absorption band at 340 mμ disappeared completely when it was incubated with the extract of mung been seedlings. The reaction was found to be stimulated by the addition of methylene blue, and the product was identified as DPN. Thus, the reaction resembled that of diaphorase, although it was less specific to the configuration of DPNH. But unlike usual diaphorase, it required a cofactor, which was neither flavins nor metallic ion, but an unidentified acidic substance. General properties of the enzyme and the cofactor are reported in this article. 相似文献
49.
Mikio Kawamori Yasushi Morikawa Yoriko Shinsha Kenichiro Takayama Seigo Takasawa 《Bioscience, biotechnology, and biochemistry》2013,77(10):2875-2879
The development of agar plate screening techniques has allowed the isolation of mutants of Trichoderma reesei capable of synthesizing cellulase under the conditions of a high concentration of glucose. Mutants resistant to catabolite repression by glycerol or glucose were isolated on Walseth’s cellulose (WC) agar plates containing 5% glycerol or 5% glucose, respectively. Mutants resistant to catabolite repression by glycerol were not derepressed enough for the production of cellulase on WC agar plates containing 5% glucose or in flask cultures with a mixture of 1% Avicel and 3% glucose. On the contrary, two mutant strains resistant to catabolite repression by glucose (KDD-10 and DGD-16) produced large clearing zones on WC agar plates containing 5% glucose. Both strains could begin to produce CMCase even in the presence of residual glucose and finally produced 1.5 times the CMCase activity, in flask cultures on 1% Avicel and 3% glucose, than that with 1% Avicel alone. These results suggest that KDD-10 and DGD-16 are comparatively derepressed by glucose for cellulase production. 相似文献
50.
Mikio Shimizu Ryo Okachi Kazuo Kimura Takashi Nara 《Bioscience, biotechnology, and biochemistry》2013,77(8):1655-1661
Penicillin acylase (EC 3.5.1.11) of Kluyvera citrophila KY7844 was purified approximately 120-fold by DEAE-cellulose chromatography, hydroxyapatite chromatography and isoelectro-focusing fractionation. The purified enzyme, with an approximate molecular weight of 63,000, appeared to be homogeneous in disc electrophoretic analysis, and showed isoelectric point (Ip) 8.12 and 13.0 units/mg of specific activity for cephalexin hydrolysis. The Michaelis constant (Km) for cephalexin and for 7-[1-(1H)-tetrazolylacetamido]-desacetoxycephalosporanic acid ((1H) T-7ADCA) was 1.4 mM and 3.6 mM, respectively. This enzyme was capable of producing (1H) T-7ADCA in 80% yield from 1-(1H)-tetrazolylacetate methylester and 7-aminodesacetoxycephalosporanic acid. 相似文献