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991.
Recent technological advances in Global Positioning System (GPS) telemetry have allowed the production of lightweight devices suitable for use on small mammals. We evaluated the use of GPS bugs on the European hedgehog (Erinaceus europaeus) in a series of static and field tests. Static tests were conducted in five different rural habitats, affording different degrees of obstruction to satellites. GPS bug performance was good in all habitats (fix success rate (FSR): median ≥?66.8 %; location error (LE): mean ≤?13.5 m), except woodland (FSR?=?37.7 %; LE?=?15.6 m), with performance highest in the open pasture habitat (FSR?=?100 %; LE?=?6.4 m). Field tests revealed mean FSR was high (84.6 %), with the use of nesting habitats, the probable cause of most failed fixes. Despite being more expensive, GPS bugs require less survey effort and substantially lower labour costs with unlimited longevity permitting re-use in multiple seasons. We recommend the use of GPS bugs in the spatial ecological study of any small mammal in a rural environment, providing accurate and unbiased movement data. Further performance testing is recommended before deployment on species inhabiting forested habitats where reduced FSR and high LE support the alternative use of very high frequency tracking. 相似文献
992.
Hannu Pöysä Jukka Rintala Aleksi Lehikoinen Risto A. Väisänen 《European Journal of Wildlife Research》2013,59(2):245-256
Populations of migratory species have undergone dramatic changes in recent decades, but little is known about the factors actually driving those changes. Of particular concern are quarry species such as migratory ducks (Anatidae), many of which have an unfavourable conservation status in Europe. By including both quarry and non-quarry species, as well as habitat preference and life history characteristics of the species, we investigated the relative importance of hunting pressure, both in Finland and at the European level, in explaining population changes of 16 species of migratory waterbirds in Finland during 1986–2011. Ban of lead shot in 1996 resulted in considerably lower annual hunting bags in Finland thereafter. Species which had the highest hunting pressure had the most negative slopes in population trends from 1986 up to 1997, suggesting that hunting probably limited those populations. However, in general population trends of the species were not strongly associated with hunting pressure in Finland or in Europe. Nor were basic life history characteristics (body mass and clutch size) associated with population trends of the species. In contrast, recent population declines were associated with habitat preferences of the species: those breeding mainly in eutrophic lakes had more negative population trends than those breeding in oligotrophic lakes or generalist species. Reasons for the relatively poor status of species preferring eutrophic lakes probably include over-eutrophication of nutrient-rich lakes, resulting in less abundant food resources, and increased nest depredation. 相似文献
993.
Marcus Bär Joachim Klaer Lothar Weinhardt Regan G. Wilks Stefan Krause Monika Blum Wanli Yang Clemens Heske Hans‐Werner Schock 《Liver Transplantation》2013,3(6):777-781
The surface properties of CuInS2 (CIS) thin‐film solar cell absorbers are investigated by a combination of electron and soft X‐ray spectroscopies. Spatially separated regions of varying colors are observed and identified to be dominated by either CuS or Cu2S surface phases. After their removal by KCN etching, the samples cannot be distinguished by eye and the CIS surface is found to be Cu‐deficient in both regions. However, a significantly more pronounced off‐stoichiometry in the region initially covered by Cu2S can be identified. In this region, the resulting surface band gap is also significantly larger than the EgSurf of the initially CuS‐terminated region. Such variations may represent a hidden parameter which, if overlooked, induces irreproducibility and thus prevents systematic optimization efforts. 相似文献
994.
Maria Bendz Marcin Skwark Daniel Nilsson Viktor Granholm Susana Cristobal Lukas Käll Arne Elofsson 《Proteomics》2013,13(9):1467-1480
Topology analysis of membrane proteins can be obtained by enzymatic shaving in combination with MS identification of peptides. Ideally, such analysis could provide quite detailed information about the membrane spanning regions. Here, we examine the ability of some shaving enzymes to provide large‐scale analysis of membrane proteome topologies. To compare different shaving enzymes, we first analyzed the detected peptides from two over‐expressed proteins. Second, we analyzed the peptides from non‐over‐expressed Escherichia coli membrane proteins with known structure to evaluate the shaving methods. Finally, the identified peptides were used to test the accuracy of a number of topology predictors. At the end we suggest that the usage of thermolysin, an enzyme working at the natural pH of the cell for membrane shaving, is superior because: (i) we detect a similar number of peptides and proteins using thermolysin and trypsin; (ii) thermolysin shaving can be run at a natural pH and (iii) the incubation time is quite short. (iv) Fewer detected peptides from thermolysin shaving originate from the transmembrane regions. Using thermolysin shaving we can also provide a clear separation between the best and the less accurate topology predictors, indicating that using data from shaving can provide valuable information when developing new topology predictors. 相似文献
995.
Jianmei Luo Meiling Chi Hongyu Wang Huanhuan He Minghua Zhou 《Bioprocess and biosystems engineering》2013,36(12):1889-1896
A convenient and promising alternative to surface modification of carbon mesh anode was fulfilled by electrochemical oxidation in the electrolyte of nitric acid or ammonium nitrate at ambient temperature. It was confirmed that such an anode modification method was low cost and effective not only in improving the efficiency of power generation in microbial fuel cells (MFCs) for synthetic wastewater treatment, but also helping to reduce the period for MFCs start-up. The MFCs with anode modification in electrolyte of nitric acid performed the best, achieving a Coulombic efficiency enhancement of 71 %. As characterized, the electrochemical modification resulted in the decrease of the anode potential and internal resistance but the increase of current response and nitrogen-containing and oxygen-containing functional groups on the carbon surface, which might contribute to the enhancement on the performances of MFCs. 相似文献
996.
Mikael Sunn?ker Alberto Giovanni Busetto Elina Numminen Jukka Corander Matthieu Foll Christophe Dessimoz 《PLoS computational biology》2013,9(1)
Approximate Bayesian computation (ABC) constitutes a class of computational methods rooted in Bayesian statistics. In all model-based statistical inference, the likelihood function is of central importance, since it expresses the probability of the observed data under a particular statistical model, and thus quantifies the support data lend to particular values of parameters and to choices among different models. For simple models, an analytical formula for the likelihood function can typically be derived. However, for more complex models, an analytical formula might be elusive or the likelihood function might be computationally very costly to evaluate. ABC methods bypass the evaluation of the likelihood function. In this way, ABC methods widen the realm of models for which statistical inference can be considered. ABC methods are mathematically well-founded, but they inevitably make assumptions and approximations whose impact needs to be carefully assessed. Furthermore, the wider application domain of ABC exacerbates the challenges of parameter estimation and model selection. ABC has rapidly gained popularity over the last years and in particular for the analysis of complex problems arising in biological sciences (e.g., in population genetics, ecology, epidemiology, and systems biology).
This is a “Topic Page” article for PLOS Computational Biology.相似文献
997.
998.
Ancient Evolutionary Trade-Offs between Yeast Ploidy States 总被引:1,自引:0,他引:1
Enik? Z?rg? Karolina Chwialkowska Arne B. Gjuvsland Elena Garré Per Sunnerhagen Gianni Liti Anders Blomberg Stig W. Omholt Jonas Warringer 《PLoS genetics》2013,9(3)
The number of chromosome sets contained within the nucleus of eukaryotic organisms is a fundamental yet evolutionarily poorly characterized genetic variable of life. Here, we mapped the impact of ploidy on the mitotic fitness of baker''s yeast and its never domesticated relative Saccharomyces paradoxus across wide swaths of their natural genotypic and phenotypic space. Surprisingly, environment-specific influences of ploidy on reproduction were found to be the rule rather than the exception. These ploidy–environment interactions were well conserved across the 2 billion generations separating the two species, suggesting that they are the products of strong selection. Previous hypotheses of generalizable advantages of haploidy or diploidy in ecological contexts imposing nutrient restriction, toxin exposure, and elevated mutational loads were rejected in favor of more fine-grained models of the interplay between ecology and ploidy. On a molecular level, cell size and mating type locus composition had equal, but limited, explanatory power, each explaining 12.5%–17% of ploidy–environment interactions. The mechanism of the cell size–based superior reproductive efficiency of haploids during Li+ exposure was traced to the Li+ exporter ENA. Removal of the Ena transporters, forcing dependence on the Nha1 extrusion system, completely altered the effects of ploidy on Li+ tolerance and evoked a strong diploid superiority, demonstrating how genetic variation at a single locus can completely reverse the relative merits of haploidy and diploidy. Taken together, our findings unmasked a dynamic interplay between ploidy and ecology that was of unpredicted evolutionary importance and had multiple molecular roots. 相似文献
999.
Mart Roosimaa Taavi Põdramägi Lumme Kadaja Arno Ruusalepp Kalju Paju Raivo Puhke Margus Eimre Ehte Orlova Andres Piirsoo Nadežda Peet Frank N. Gellerich Enn Seppet 《Mitochondrion》2013,13(5):399-409
Cardiac energy metabolism with emphasis on mitochondria was addressed in atrial tissue from patients with overload-induced atrial dilation. Structural remodeling of dilated (D) atria manifested as intracellular accumulation of fibrillar aggregates, lipofuscin, signs of myolysis and autophagy. Despite impaired complex I dependent respiration and increased diffusion restriction for ADP, no changes regarding adenylate and creatine kinase occurred. We observed 7-fold overexpression of HK2 gene in D atria with concomitant 2-fold greater activation of mitochondrial oxygen consumption by glucose, which might represent an adaption to increased energy requirements and impaired mitochondrial function by effectively joining glycolysis and oxidative phosphorylation. 相似文献
1000.