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491.
492.
Bordoloi Devivasha Padmavathi Ganesan Banik Kishore Devi Khwairakpam Amrita Harsha Choudhary Girisa Sosmitha Buhrmann Constanze Shakibaei Mehdi Kunnumakkara Ajaikumar B. 《Molecular and cellular biochemistry》2021,476(9):3303-3318
Molecular and Cellular Biochemistry - Lung cancer represents one of the most prevalent neoplasms across the globe. Tobacco smoking, exposure to different occupational and environmental carcinogens,... 相似文献
493.
Punithavathi Ranganathan Calpurnia Jayakumar Dean Y. Li Ganesan Ramesh 《Journal of cellular and molecular medicine》2014,18(7):1290-1299
The netrin-1 administration or overexpression is known to protect colon from acute colitis. However, the receptor that mediates netrin-1 protective activities in the colon during colitis remains unknown. We tested the hypothesis that UNC5B receptor is a critical mediator of protective function of netrin-1 in dextran sodium sulfate (DSS)-induced colitis using mice with partial deletion of UNC5B receptor. DSS colitis was performed in mice with partial genetic UNC5B deficiency (UNC5B+/− mice) or wild-type mice to examine the role of endogenous UNC5B. These studies were supported by in vitro models of DSS-induced apoptosis in human colon epithelial cells. WT mice developed colitis in response to DSS feeding as indicated by reduction in bw, reduction in colon length and increase in colon weight. These changes were exacerbated in heterozygous UNC5B knockout mice treated with DSS. Periodic Acid-Schiff stained section shows damages in colon epithelium and mononuclear cell infiltration in WT mice, which was further increased in UNC5B heterozygous knockout mice. This was associated with large increase in inflammatory mediators such as cytokine and chemokine expression and extensive apoptosis of epithelial cells in heterozygous knockout mice as compared to WT mice. Overexpression of UNC5B human colon epithelial cells suppressed DSS-induced apoptosis and caspase-3 activity. Moreover, DSS induced large amount of netrin-1 and shRNA mediated knockdown of netrin-1 induction exacerbated DSS-induced epithelial cell apoptosis. Our results suggest that UNC5B is a critical mediator of cell survival in response to stress in colon. 相似文献
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495.
Trayambak Basak Gaurav Garg Nitin Bhardwaj Vinay Singh Tanwar Sandeep Seth Ganesan Karthikeyan 《Biomarkers》2016,21(5):436-440
Coronary artery disease (CAD) has been increasing alarmingly in India. We had earlier shown that vitamin B12 deficiency is associated with CAD in Indian population. However, only about a quarter of the total vitamin B12 is internalised in the cells by the proteins transcobalamin II. Vitamin B12-bound transcobalamin II (holotranscobalamin, holoTC) is thus referred to as biologically active B12. In this study, we ascertained the levels of holoTC in 501 CAD cases and 1253 healthy controls and for the first time show that holoTC levels are significantly lower (p?=?2.57E-4) in CAD (26.81?pmol/l) cases as compared to controls (29.97?pmol/l). 相似文献
496.
Boopathi M Suryanarayana MV Nigam AK Pandey P Ganesan K Singh B Sekhar K 《Biosensors & bioelectronics》2006,21(12):2339-2344
Molecularly imprinted polymers (MIPs) known as plastic antibodies (PAs) represent a new class of materials possessing high selectivity and affinity for the target molecule. Since their discovery, PAs have attracted considerable interest from bio- and chemical laboratories to pharmaceutical institutes. PAs are becoming an important class of synthetic materials mimicking molecular recognition by natural receptors. In addition, they have been utilized as catalysts, sorbents for solid-phase extraction, stationary phase for liquid chromatography and mimics of enzymes. In this paper, first time we report the preparation and characterization of a PA for the recognition of blistering chemical warfare agent sulphur mustard (SM). The SM imprinted PA exhibited more surface area when compared to the control non-imprinted polymer (NIP). In addition, SEM image showed an ordered nano-pattern for the PA of SM that is entirely different from the image of NIP. The imprinting also enhanced SM rebinding ability to the PA when compared to the NIP with an imprinting efficiency () of 1.3. 相似文献
497.
A procedure for the synthesis of N-succinimidyl 3-iodobenzoate labeled with any iodine isotope ([*I]SIB), which is an agent used in the radioiodination of proteins and peptides, from its tin precursor N-succinimidyl 3-(tri-n-butylstannyl)benzoate (STB) is described. Also included are protocols for the synthesis of an unlabeled standard of SIB and the tin precursor. Radioiododestannylation of STB using tert-butylhydroperoxide as the oxidant gives [*I]SIB in 80% radiochemical yields. The total time for the synthesis of [*I]SIB from STB is approximately 95 min. Use of [*I]SIB yields radioiodinated proteins that are considerably more stable in vivo than those radioiodinated by the direct electrophilic method. 相似文献
498.
Ganesan Pugalenthi Krishna Kumar Kandaswamy P. N. Suganthan G. Archunan R. Sowdhamini 《Amino acids》2010,39(3):777-783
Lipocalins are functionally diverse proteins that are composed of 120–180 amino acid residues. Members of this family have
several important biological functions including ligand transport, cryptic coloration, sensory transduction, endonuclease
activity, stress response activity in plants, odorant binding, prostaglandin biosynthesis, cellular homeostasis regulation,
immunity, immunotherapy and so on. Identification of lipocalins from protein sequence is more challenging due to the poor
sequence identity which often falls below the twilight zone. So far, no specific method has been reported to identify lipocalins
from primary sequence. In this paper, we report a support vector machine (SVM) approach to predict lipocalins from protein
sequence using sequence-derived properties. LipoPred was trained using a dataset consisting of 325 lipocalin proteins and
325 non-lipocalin proteins, and evaluated by an independent set of 140 lipocalin proteins and 21,447 non-lipocalin proteins.
LipoPred achieved 88.61% accuracy with 89.26% sensitivity, 85.27% specificity and 0.74 Matthew’s correlation coefficient (MCC).
When applied on the test dataset, LipoPred achieved 84.25% accuracy with 88.57% sensitivity, 84.22% specificity and MCC of
0.16. LipoPred achieved better performance rate when compared with PSI-BLAST, HMM and SVM-Prot methods. Out of 218 lipocalins,
LipoPred correctly predicted 194 proteins including 39 lipocalins that are non-homologous to any protein in the SWISSPROT
database. This result shows that LipoPred is potentially useful for predicting the lipocalin proteins that have no sequence
homologs in the sequence databases. Further, successful prediction of nine hypothetical lipocalin proteins and five new members
of lipocalin family prove that LipoPred can be efficiently used to identify and annotate the new lipocalin proteins from sequence
databases. The LipoPred software and dataset are available at . 相似文献
499.
Real-world datasets commonly have issues with data imbalance. There are several approaches such as weighting, sub-sampling,
and data modeling for handling these data. Learning in the presence of data imbalances presents a great challenge to machine
learning. Techniques such as support-vector machines have excellent performance for balanced data, but may fail when applied
to imbalanced datasets. In this paper, we propose a new undersampling technique for selecting instances from the majority
class. The performance of this approach was evaluated in the context of several real biological imbalanced data. The ratios
of negative to positive samples vary from ~9:1 to ~100:1. Useful classifiers have high sensitivity and specificity. Our results
demonstrate that the proposed selection technique improves the sensitivity compared to weighted support-vector machine and
available results in the literature for the same datasets. 相似文献
500.