全文获取类型
收费全文 | 918篇 |
免费 | 53篇 |
专业分类
971篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 19篇 |
2021年 | 12篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 20篇 |
2017年 | 19篇 |
2016年 | 22篇 |
2015年 | 43篇 |
2014年 | 36篇 |
2013年 | 70篇 |
2012年 | 58篇 |
2011年 | 77篇 |
2010年 | 48篇 |
2009年 | 40篇 |
2008年 | 56篇 |
2007年 | 60篇 |
2006年 | 63篇 |
2005年 | 59篇 |
2004年 | 64篇 |
2003年 | 59篇 |
2002年 | 50篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 10篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1986年 | 2篇 |
1981年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有971条查询结果,搜索用时 0 毫秒
961.
Kitagawa Hiroshi; Tsutsumi Kae; Ujikawa Miho; Goto Fumitaka; Tamura Jun-ichi; Neumann Klaus W.; Ogawa Tomoya; Sugahara Kazuyuki 《Glycobiology》1997,7(4):531-537
The relationship between sulfation and polymerization in chondroitinsulfate (CS) biosynthesis has been poorly understood. In thisstudy, we investigated the specificity of bovine serum UDP-GalNAc:CS ß-GalNAc transferase responsible for chain elongationusing structurally defined acceptor substrates. They consistedof tetra- and hexasaccharide-serines that were chemically synthesizedand various regular oligosaccharides with a GlcA residue atthe nonreducing terminus, prepared from chondroitin and CS usingtesticular hyaluronidase. The enzyme preparation was obtainedfrom fetal bovine serum by means of heparin-Sepharose affinitychromatography. The preparation did not contain the 相似文献
962.
Miho Sakai Andreas Vonderheit Xun Wei Claudia Küttel Andreas Stemmer 《Biosensors & bioelectronics》2009,25(1):68-75
Macrophage phagocytosis activates NADPH oxidase, an electron transport system in the plasma membrane. This study examined the feasibility of utilizing electrons transferred through the plasma membrane via NADPH oxidase to run a biofuel cell. THP-1 human monocytic cells were chemically stimulated to differentiate into macrophages. Further they were activated to induce a phagocytic response. During differentiation, cells became adherent to a plain gold electrode which was used as anode in a two-compartment fuel cell system. The current production in the fuel cell always corresponded to the NADPH oxidase activity, which was evaluated by the amount of superoxide anion produced upon stimulation in combination with the expression levels of the different NADPH oxidase subunits in cells. Moreover, our results of different inhibitory tests let us conclude that (i) the current observed in the fuel cell originates from NADPH oxidase in activated macrophages and (ii) there are multiple electron transport pathways from the cells to the electrode. One pathway involves superoxide anions produced upon stimulation, additional not yet identified electron transport occurs independently of superoxide anions.This type of novel biofuel cell driven by living human cells may eventually develop into a battery replacement for small medical devices. 相似文献
963.
964.
965.
966.
LA Wistar rats have a deficiency of androsterone UDP-glucuronosyltransferase (UDPGT) and are present in Wistar rat colonies around the world. In order to clarify the molecular mechanism of the deficiency, androsterone UDPGT cDNA clone, pGT2 was isolated from rat liver cDNA library and was digested with restriction enzymes to afford three probes for Northern and Southern blot analyses in HA (normal), heterozygous LA and LA Wistar rats. In Northern blot analysis, androsterone UDPGT mRNA was totally absent in LA Wistar rat liver. Southern blot analysis suggested a large deletion of androsterone UDPGT gene in the rats. Genomic DNA amplifications with synthetic primers which have nucleotide sequences corresponding to the 5′-region of androsterone UDPGT cDNA, suggested that androsterone UDPGT gene has exon 1 with a length of some 700 bp and that this exon is deleted in LA Wistar rats. Based on these lines of evidence, it is concluded that the large portion of androsterone UDPGT gene is deleted in LA Wistar rats, which results in the absence of androsterone UDPGT mRNA and consequently the corresponding enzyme protein. 相似文献
967.
Miho Aoshima Yoshihisa Nishibe Masami Hasegawa Akihiko Yamagishi Tairo Oshima 《Gene》1996,180(1-2):183-187
A hyperthermophile NC12 was newly isolated from Noboribetsu hot spring. To characterize this organism, a gene coding for 16S rRNA was cloned and sequenced. The 16S rRNA sequence from NC12 shows the highest similarity with those from Pyrodictium occultum and Desulfurococcus mobilis among the sequences in the database, indicating that NC12 belongs to a cluster of extreme thermophiles (Crenarchaeota) in the archaeal domain. However, since the highest identity score was only 91.2%, it is suggested that NC12 may constitute a new genus. 相似文献
968.
969.
Keiichi Tsukahara Kimiko Kiguchi Miho Matsui Naoko Kubota Ryuichi Arakawa Takeshi Sakurai 《Journal of biological inorganic chemistry》2000,5(6):765-773
Horse heart metmyoglobins modified with diethylenetriaminepentaacetic acid, metMb(DTPA)n (n=1, 2, 4, and 5), were characterized by a MALDI-TOF mass spectrometry, amino-acid sequence analysis, and UV-Vis and CD spectroscopies. The DTPA-binding sites on metMb were Lys47, Lys50, Lys87, Lys145, and Lys147 for metMb(DTPA)5, Lys47, Lys87, Lys145, and Lys147 for metMb(DTPA)4, Lys87 and Lys145 for metMb(DTPA)2, and Lys87 for metMbDTPA, respectively. The modified metMb(DTPA)n showed cytochrome c peroxidase-like activity more efficiently than native metMb: metMb(DTPA)5>metMb(DTPA)4>metMb(DTPA)2> metMbDTPA approximately equals native metMb. The first-order rate constants for the reactions of ferrylMb(DTPA)n (n=2, 4, and 5) with reduced cytochrome c [cyt c(II)] were saturated with concentrations of cyt c(II), suggesting that the electron transfer (ET) occurs within a diprotein complex. The intramolecular ET rate constants in the diprotein complex increased with increasing the number of DTPA ions. The reactions of native ferrylMb and ferrylMbDTPA with cyt c(II) obeyed a second-order rate law. A possible ET mechanism is proposed; cyt c(II) binds the DTPA-linked anionic patch around Lys87, Lys145, and Lys147 region of ferrylMb(DTPA)n. 相似文献
970.
Noriyuki Kasai Shinji Hirosue Hirofumi Ichinose Miho Ohta 《Biochemical and biophysical research communications》2009,387(1):103-123
We cloned full-length cDNAs of more than 130 cytochrome P450s (P450s) derived from Phanerochaete chrysosporium, and successfully expressed 70 isoforms using a co-expression system of P. chrysosporium P450 and yeast NADPH-P450 reductase in Saccharomyces cerevisiae. Of these P450s, a microsomal P450 designated as PcCYP65a2 consists of 626 amino acid residues with a molecular mass of 68.3 kDa. Sequence alignment of PcCYP65a2 and human CYP1A2 revealed a unique structure of PcCYP65a2. Functional analysis of PcCYP65a2 using the recombinant S. cerevisiae cells demonstrated that this P450 catalyzes 3′-hydroxylation of naringenin to yield eriodictyol, which has various biological and pharmacological properties. In addition, the recombinant S. cerevisiae cells expressing PcCYP65a2 metabolized such polyaromatic compounds as dibenzo-p-dioxin (DD), 2-monochloroDD, biphenyl, and naphthalene. These results suggest that PcCYP65a2 is practically useful for both bioconversion and bioremediation. 相似文献