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21.
Christine P. Diggle Daniel J. Moore Girish Mali Petra zur Lage Aouatef Ait-Lounis Miriam Schmidts Amelia Shoemark Amaya Garcia Munoz Mihail R. Halachev Philippe Gautier Patricia L. Yeyati David T. Bonthron Ian M. Carr Bruce Hayward Alexander F. Markham Jilly E. Hope Alex von Kriegsheim Hannah M. Mitchison Ian J. Jackson Bénédicte Durand Walter Reith Eamonn Sheridan Andrew P. Jarman Pleasantine Mill 《PLoS genetics》2014,10(9)
22.
Shixin Yang Lucian Barbu-Tudoran Marek Orzechowski Roger Craig John Trinick Howard White William Lehman 《Biophysical journal》2014,106(4):855-864
Muscle contraction is regulated by troponin-tropomyosin, which blocks and unblocks myosin binding sites on actin. To elucidate this regulatory mechanism, the three-dimensional organization of troponin and tropomyosin on the thin filament must be determined. Although tropomyosin is well defined in electron microscopy helical reconstructions of thin filaments, troponin density is mostly lost. Here, we determined troponin organization on native relaxed cardiac muscle thin filaments by applying single particle reconstruction procedures to negatively stained specimens. Multiple reference models led to the same final structure, indicating absence of model bias in the procedure. The new reconstructions clearly showed F-actin, tropomyosin, and troponin densities. At the 25 Å resolution achieved, troponin was considerably better defined than in previous reconstructions. The troponin density closely resembled the shape of troponin crystallographic structures, facilitating detailed interpretation of the electron microscopy density map. The orientation of troponin-T and the troponin core domain established troponin polarity. Density attributable to the troponin-I mobile regulatory domain was positioned where it could hold tropomyosin in its blocking position on actin, thus suggesting the underlying structural basis of thin filament regulation. Our previous understanding of thin filament regulation had been limited to known movements of tropomyosin that sterically block and unblock myosin binding sites on actin. We now show how troponin, the Ca2+ sensor, may control these movements, ultimately determining whether muscle contracts or relaxes. 相似文献
23.
Antos Shakhbazau Dzmitry Shcharbin Ihar Seviaryn Natalya Goncharova Svetlana Kosmacheva Mihail Potapnev Barbara Gabara Maxim Ionov Maria Bryszewska 《Molecular biology reports》2010,37(4):2003-2008
We report the use of polyamidoamine (PAMAM-NH2) dendrimers along with other non-viral vehicles for the in vitro transfection of human bone marrow mesenchymal stem cells
(hMSCs) and for engineering MSCs to secrete brain-derived neurotrophic factor (BDNF). Different generations of cationic polyamidoamine
dendrimers (generations 3–6) were tested on HEK 293T cells. hMSCs were then transfected with PAMAM-NH2 G4 dendrimers and Lipofectamine 2000, which elicited the expression of GFP reporter in around 6 and 20% of the cells, respectively.
Both vehicles were then shown to elicit the expression of BDNF in MSCs from a bicistronic cassette. Non-virally induced neurotrophin
expression may be a safe and easy method for adapting autologous stem cells for therapeutic treatment of diseases and neural
system injuries. 相似文献
24.
Leaf rust induced volatile organic compounds signalling in willow during the infection 总被引:1,自引:0,他引:1
Merje Toome Pille Randjärv Lucian Copolovici Ülo Niinemets Katrin Heinsoo Anne Luik Steffen M. Noe 《Planta》2010,232(1):235-243
Plants are known to emit volatile organic compounds (VOC) in response to various biotic or abiotic stresses. Although the
VOC emission in the case of insect attacks is well described, there is only little known about the impact of pathogens on
plant emission. In the present study, we used a willow-leaf rust system to describe the effects of a biotrophic fungal infection
on the VOC emission pattern of willow leaves. We detected that isoprene emissions from rust-infected leaves decreased threefold
compared to control. The total monoterpene emissions did not change although a stress-signalling compound (Z)-β-ocimene showed an increase in infected plants on several days. The infection also increased the emission of sesquiterpenes
and lipoxygenase products (LOX) by factors of 175-fold and 10-fold, respectively. The volatile emission signals showed two
clear peaks during the experiment. At 6, 7 and 12 days post-infection (dpi), the relative volatile emission signal increased
to about sixfold compared to uninfected plants. These time points are directly connected to rust infection since at 6 dpi
the first rust pustules appeared on the leaves and at 12 dpi necrosis had developed around several pustules. We present correlations
between LOX and sesquiterpene emission signals, which suggest at least two different steps in eliciting the volatile emission. 相似文献
25.
Caraş I Tucureanu C Lerescu L Sălăgeanu A 《Roumanian archives of microbiology and immunology》2007,66(1-2):5-9
Dendritic cells (DCs) play a pivotal role in linking innate and adaptive immunity. Migration to the lymph nodes and maturation of DCs are crucial steps in the initiation of specific immune responses. The bacterial product CANTASTIM (CS) is a purified extract of Pseudomonas aeruginosa that induces non-specific protection against bacterial infection, enhances macrophage effector functions and modulates cytokines production. In this study, we used a mouse skin explant culture model and human monocyte-derived DCs to study the effect of CS on the migration and maturation of DCs, respectively. We noticed a significant increase in the number of DCs which migrated from the skin explants when CS was added to the culture medium. Also, CS was able to induce the expression of maturation-associated marker CD83 on human monocyte-derived DCs. DC-based tumor vaccines represent a promising approach for cancer immunotherapy and the migration rate and maturation state of DCs are important parameters for their clinical effectiveness. CS may be an attractive candidate to be tested for the production of DC-based vaccine. 相似文献
26.
Neurochemical Research - Ferulago angulata (Apiaceae) is a shrub indigenous to western Iran, Turkey and Iraq. In traditional medicine, F. angulata is recommended for treating digestive pains,... 相似文献
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The RNA-binding protein Arrest (Bruno) regulates alternative splicing to enable myofibril maturation in Drosophila flight muscle 下载免费PDF全文
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Davis JL Huang L Worodria W Masur H Cattamanchi A Huber C Miller C Conville PS Murray P Kovacs JA 《PloS one》2011,6(1):e16321