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排序方式: 共有436条查询结果,搜索用时 13 毫秒
71.
Constantina Heltianu Simona-Adriana Manea Cristian Guja Carina Mihai Constantin Ionescu-Tirgoviste 《Central European Journal of Biology》2008,3(3):243-249
Advanced glycation end products (AGEs) are involved in the occurrence of vascular complications in diabetes. The present study
was undertaken to investigate the level of low-molecular weight products of AGEs (LMW-AGEs) in relation to microvascular complications
in type 1 diabetes, and the possible relationship with nitric oxide (NO) as a marker of endothelial function. Patients with
normal renal function (NRF) were classified into two groups: (1) without, and (2) with diabetic neuropathy; and patients with
renal impairment also into two groups: (3) diabetic renal disease, and (4) end-stage renal disease. The fluorescence of LMW-AGEs
and measurement of NO metabolites was assessed in 277 serum samples. In addition, multiple regression analysis was performed.
In group 1, LMW-AGEs level (9.3±1.1 AF%) was higher than in the control group (2.4±0.3 AF%). A trend in the increase of LMW-AGEs
with neuropathy (29.7±5.5 AF%, group 2), and further with renal impairment (47.0±8.0, group 3 and 137.8±25.5 AF%, group 4),
was observed. In multivariate regression analysis LMW-AGEs were associated with NO metabolites in group 2. In NRF patients,
diabetic neuropathy was significantly correlated with LMW-AGEs and NO metabolites, independently of serum creatinine and duration
of diabetes. This relationship suggests that the NO and LMW-AGEs’ actions (possibly synergistic) in endothelial activation
possess a role in the initiation and development of diabetic microvascular complications. 相似文献
72.
Priya A. Debisarun Katharina L. Gssling Ozlem Bulut Gizem Kilic Martijn Zoodsma Zhaoli Liu Marina Oldenburg Nadine Rüchel Bowen Zhang Cheng-Jian Xu Patrick Struycken Valerie A. C. M. Koeken Jorge Domínguez-Andrs Simone J. C. F. M. Moorlag Esther Taks Philipp N. Ostermann Lisa Müller Heiner Schaal Ortwin Adams Arndt Borkhardt Jaap ten Oever Reinout van Crevel Yang Li Mihai G. Netea 《PLoS pathogens》2021,17(10)
73.
Lisa Rizzetto Daniela C. Ifrim Silvia Moretti Noemi Tocci Shih-Chin Cheng Jessica Quintin Giorgia Renga Vasilis Oikonomou Carlotta De Filippo Tobias Weil Bastiaan A. Blok Marcello S. Lenucci Manuel A. S. Santos Luigina Romani Mihai G. Netea Duccio Cavalieri 《The Journal of biological chemistry》2016,291(15):7961-7972
The immune system is essential to maintain the mutualistic homeostatic interaction between the host and its micro- and mycobiota. Living as a commensal, Saccharomyces cerevisiae could potentially shape the immune response in a significant way. We observed that S. cerevisiae cells induce trained immunity in monocytes in a strain-dependent manner through enhanced TNFα and IL-6 production upon secondary stimulation with TLR ligands, as well as bacterial and fungal commensals. Differential chitin content accounts for the differences in training properties observed among strains, driving induction of trained immunity by increasing cytokine production and direct antimicrobial activity both in vitro and in vivo. These chitin-induced protective properties are intimately associated with its internalization, identifying a critical role of phagosome acidification to facilitate microbial digestion. This study reveals how commensal and passenger microorganisms could be important in promoting health and preventing mucosal diseases by modulating host defense toward pathogens and thus influencing the host microbiota-immune system interactions. 相似文献
74.
Elena Topchiy Mihai Cirstea HyeJin Julia Kong John H. Boyd Yingjin Wang James A. Russell Keith R. Walley 《PloS one》2016,11(5)
Sepsis is the leading cause of death in critically ill patients. While decreased Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) function improves clinical outcomes in murine and human sepsis, the mechanisms involved have not been fully elucidated. We tested the hypothesis that lipopolysaccharide (LPS), the major Gram-negative bacteria endotoxin, is cleared from the circulation by hepatocyte Low Density Lipoprotein Receptors (LDLR)—receptors downregulated by PCSK9. We directly visualized LPS uptake and found that LPS is rapidly taken up by hepatocytes into the cell periphery. Over the course of 4 hours LPS is transported towards the cell center. We next found that clearance of injected LPS from the blood was reduced substantially in Ldlr knockout (Ldlr-/-) mice compared to wild type controls and, simultaneously, hepatic uptake of LPS was also reduced in Ldlr-/- mice. Specifically examining the role of hepatocytes, we further found that primary hepatocytes isolated from Ldlr-/- mice had greatly decreased LPS uptake. In the HepG2 immortalized human hepatocyte cell line, LDLR silencing similarly resulted in decreased LPS uptake. PCSK9 treatment reduces LDLR density on hepatocytes and, therefore, was another independent strategy to test our hypothesis. Incubation with PCSK9 reduced LPS uptake by hepatocytes. Taken together, these findings demonstrate that hepatocytes clear LPS from the circulation via the LDLR and PCSK9 regulates LPS clearance from the circulation during sepsis by downregulation of hepatic LDLR. 相似文献
75.
76.
Nucleotide-binding oligomerization domain-2 modulates specific TLR pathways for the induction of cytokine release 总被引:13,自引:0,他引:13
Netea MG Ferwerda G de Jong DJ Jansen T Jacobs L Kramer M Naber TH Drenth JP Girardin SE Kullberg BJ Adema GJ Van der Meer JW 《Journal of immunology (Baltimore, Md. : 1950)》2005,174(10):6518-6523
The recognition of peptidoglycan by cells of the innate immune system has been controversial; both TLR2 and nucleotide-binding oligomerization domain-2 (NOD2) have been implicated in this process. In the present study we demonstrate that although NOD2 is required for recognition of peptidoglycan, this leads to strong synergistic effects on TLR2-mediated production of both pro- and anti-inflammatory cytokines. Defective IL-10 production in patients with Crohn's disease bearing loss of function mutations of NOD2 may lead to overwhelming inflammation due to a subsequent Th1 bias. In addition to the potentiation of TLR2 effects, NOD2 is a modulator of signals transmitted through TLR4 and TLR3, but not through TLR5, TLR9, or TLR7. Thus, interaction between NOD2 and specific TLR pathways may represent an important modulatory mechanism of innate immune responses. 相似文献
77.
Divorce between breeding seasons, i.e. mate change while the old breeding partner is still alive, has been well-studied in
several bird species. It can be viewed either as the result of reproductive decisions of individuals to maximize their own
fitness or as the outcome of intra-sexual competition for mating opportunities. In contrast, divorce within a breeding season—also
referred to as rapid mate switching—has received much less attention. Within-season divorce may occur after sudden changes
in environmental conditions, such as those causing the disappearance or devaluation of nesting sites or territories, or when
better breeding partners become available. Within-season divorce can be initiated by a member of the pair, or it can be the
result of a take-over by an unpaired individual that competes for access to the resource. During a field experiment investigating
the effects of limiting nesting sites on reproductive behaviour in Blue Tits, we recorded several cases of within-season divorce.
The rate of divorce was not related to the experimental nest-site limitation, and pairs that changed their partner suffered
reduced reproductive success compared to faithful pairs. Although there were no differences in the timing of breeding, clutch
size or hatching success, pairs with a new partner also suffered a reduced fledging success, which was partly explained by
complete brood failures. This study highlights that the pair bond prior to egg laying can be unstable when conditions force
individuals to compete for a new partner or nest site and indicates the importance of the correct timing of divorce within
the breeding cycle. 相似文献
78.
79.
Ben Langmead Michael C Schatz Jimmy Lin Mihai Pop Steven L Salzberg 《Genome biology》2009,10(11):R134-10
As DNA sequencing outpaces improvements in computer speed, there is a critical need to accelerate tasks like alignment and
SNP calling. Crossbow is a cloud-computing software tool that combines the aligner Bowtie and the SNP caller SOAPsnp. Executing
in parallel using Hadoop, Crossbow analyzes data comprising 38-fold coverage of the human genome in three hours using a 320-CPU
cluster rented from a cloud computing service for about $85. Crossbow is available from . 相似文献
80.
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome 总被引:20,自引:0,他引:20
Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the
human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint
of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques with a novel quality-aware backtracking
algorithm that permits mismatches. Multiple processor cores can be used simultaneously to achieve even greater alignment speeds.
Bowtie is open source . 相似文献