全文获取类型
收费全文 | 565篇 |
免费 | 53篇 |
专业分类
618篇 |
出版年
2023年 | 4篇 |
2022年 | 9篇 |
2021年 | 9篇 |
2020年 | 14篇 |
2019年 | 13篇 |
2018年 | 13篇 |
2017年 | 9篇 |
2016年 | 30篇 |
2015年 | 41篇 |
2014年 | 24篇 |
2013年 | 45篇 |
2012年 | 51篇 |
2011年 | 68篇 |
2010年 | 39篇 |
2009年 | 27篇 |
2008年 | 32篇 |
2007年 | 41篇 |
2006年 | 32篇 |
2005年 | 36篇 |
2004年 | 22篇 |
2003年 | 21篇 |
2002年 | 14篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1998年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1984年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1974年 | 2篇 |
1969年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有618条查询结果,搜索用时 21 毫秒
611.
612.
613.
Mihaela Gheorghiu Andreea Olaru Aurelia Tar Cristina Polonschii Eugen Gheorghiu 《Biosensors & bioelectronics》2009,24(12):3517-3523
A new and exciting biosensing avenue based on assessment of the non-monotonous, concentration dependent effect of pore formation is discussed. A novel kinetic model is advanced to relate surface plasmon resonance (SPR) data with actual concentrations of interacting partners. Lipid modified L1 sensor chip provide the accessible platform for SPR exploration of peptide–membrane interaction, with POPC and melittin as model systems. We show that quantitative assessment of the interaction between an antimicrobial peptide and lipid modified sensors is capable to provide both sensing avenues and detailed mechanistic insights into effects of pore-forming compounds. The proposed model combined with appropriate design of the experimental protocol adds a new depth to the classic SPR investigation of peptide–lipid interaction offering a quantitative platform for detection, improved understanding of the manifold facets of the interaction and for supporting the controlled design of novel antimicrobial compounds. This biosensing approach can be applied to an entire set of pore-forming compounds including antimicrobial peptides and exo-toxins. 相似文献
614.
Mihaela C. Balasu Laurentiu N. Spiridon Simona Miron Constantin T. Craescu Axel J. Scheidig Andrei-Jose Petrescu Stefan E. Szedlacsek 《PloS one》2009,4(5)
The activity of ERK2, an essential component of MAP-kinase pathway, is under the strict control of various effector proteins. Despite numerous efforts, no crystal structure of ERK2 complexed with such partners has been obtained so far. PTP-SL is a major regulator of ERK2 activity. To investigate the ERK2–PTP-SL complex we used a combined method based on cross-linking, MALDI-TOF analysis, isothermal titration calorimetry, molecular modeling and docking. Hence, new insights into the stoichiometry, thermodynamics and interacting regions of the complex are obtained and a structural model of ERK2-PTP-SL complex in a state consistent with PTP-SL phosphatase activity is developed incorporating all the experimental constraints available at hand to date. According to this model, part of the N-terminal region of PTP-SL has propensity for intrinsic disorder and becomes structured within the complex with ERK2. The proposed model accounts for the structural basis of several experimental findings such as the complex-dissociating effect of ATP, or PTP-SL blocking effect on the ERK2 export to the nucleus. A general observation emerging from this model is that regions involved in substrate binding in PTP-SL and ERK2, respectively are interacting within the interface of the complex. 相似文献
615.
The white-rot fungus Trametes versicolor decolorized Amaranth. The hypothesis that the carbon structure of Amaranth was broken down in smaller mass fragments was
investigated analyzing the products of decoloration. FTIR spectroscopy, ion chromatography, sulfite and ammonia analysis were
used to compare the culture filtrate before dye addition, with the pure dye, the culture filtrate after dye addition, and
the culture filtrate during the treatment. The hypothesis of polymerization of the decoloration products was tested by spectrophotometric
analysis of dialysates of the pure dye, the culture filtrate before dye addition, and the culture filtrates after dye addition
and decoloration. FTIR showed that the signals typical for the azo group disappeared after decoloration, while new peaks appeared
that were characteristic of substituted naphthalenic or benzenic compounds. Ion chromatography showed that the level of sulfate
in the treatment increased when compared with the level of the sulfate in control, suggesting that the sulfonic groups were
being stripped from Amaranth’s structure and metabolized to sulfate. Sulfite measurements for the treatment and controls showed
no significant difference, and were well below the saturation concentration for sulfite in water, confirming that the medium
was aerobic. Ammonia concentration did not change with the decoloration. Absorbance scans after dialysis of decolorized samples
showed no new peaks, suggesting that the decoloration products were not polymerized. These observations suggests that the
decoloration mechanism starts with the azo link removal, followed by desulfonation, naphthalene ring opening, and the formation
of smaller mass fragments, similar to fungal metabolites. 相似文献
616.
Mihaela Škulj Veronika Okršlar Špela Jalen Simona Jevševar Petra Slanc Borut Štrukelj Viktor Menart 《Microbial cell factories》2008,7(1):6
Background
Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was recognised that a method for accurate and rapid quantification of plasmid copy number is necessary for optimization and better understanding of this process. Lately, qPCR is becoming the method of choice for this purpose. In the presented work, an improved qPCR method adopted for PCN determination in various fermentation processes was developed. 相似文献617.
618.
S Goldstein M Moreau V Barbu M Meier E Alsat C Mazière J C Mazière J Polonovski 《Biochimie》1989,71(3):379-382
Polyclonal antibodies were prepared by immunization of rabbits with partially purified LDL receptor obtained from human placental microvilli. The antiserum reacted with membranes from human placental microvilli and human fibroblasts, as assessed by immunobinding studies. It also reacted with purified LDL receptors of both origins. The antiserum markedly inhibited 125I-labeled LDL binding to cultured human fibroblasts. 相似文献