全文获取类型
收费全文 | 20340篇 |
免费 | 1361篇 |
国内免费 | 9篇 |
专业分类
21710篇 |
出版年
2023年 | 163篇 |
2022年 | 208篇 |
2021年 | 431篇 |
2020年 | 366篇 |
2019年 | 388篇 |
2018年 | 632篇 |
2017年 | 587篇 |
2016年 | 799篇 |
2015年 | 1208篇 |
2014年 | 1219篇 |
2013年 | 1526篇 |
2012年 | 1810篇 |
2011年 | 1557篇 |
2010年 | 1048篇 |
2009年 | 806篇 |
2008年 | 1200篇 |
2007年 | 1153篇 |
2006年 | 1032篇 |
2005年 | 901篇 |
2004年 | 827篇 |
2003年 | 727篇 |
2002年 | 717篇 |
2001年 | 270篇 |
2000年 | 230篇 |
1999年 | 184篇 |
1998年 | 164篇 |
1997年 | 110篇 |
1996年 | 110篇 |
1995年 | 115篇 |
1994年 | 83篇 |
1993年 | 82篇 |
1992年 | 112篇 |
1991年 | 101篇 |
1990年 | 77篇 |
1989年 | 65篇 |
1988年 | 73篇 |
1987年 | 55篇 |
1986年 | 48篇 |
1985年 | 63篇 |
1984年 | 51篇 |
1983年 | 34篇 |
1982年 | 40篇 |
1981年 | 38篇 |
1980年 | 28篇 |
1979年 | 31篇 |
1978年 | 32篇 |
1977年 | 29篇 |
1975年 | 21篇 |
1974年 | 27篇 |
1973年 | 17篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
51.
52.
We describe a unique mitochondrial oscillator that depends on oxidative phosphorylation, reactive oxygen species (ROS), and mitochondrial inner membrane ion channels. Cell-wide synchronized oscillations in mitochondrial membrane potential (Delta Psi(m)), NADH, and ROS production have been recently described in isolated cardiomyocytes, and we have hypothesized that the balance between superoxide anion efflux through inner membrane anion channels and the intracellular ROS scavenging capacity play a key role in the oscillatory mechanism. Here, we formally test the hypothesis using a computational model of mitochondrial energetics and Ca(2+) handling including mitochondrial ROS production, cytoplasmic ROS scavenging, and ROS activation of inner membrane anion flux. The mathematical model reproduces the period and phase of the observed oscillations in Delta Psi(m), NADH, and ROS. Moreover, we experimentally verify model predictions that the period of the oscillator can be modulated by altering the concentration of ROS scavengers or the rate of oxidative phosphorylation, and that the redox state of the glutathione pool oscillates. In addition to its role in cellular dysfunction during metabolic stress, the period of the oscillator can be shown to span a wide range, from milliseconds to hours, suggesting that it may also be a mechanism for physiological timekeeping and/or redox signaling. 相似文献
53.
Dinis-Oliveira RJ Remião F Duarte JA Ferreira R Sánchez Navarro A Bastos ML Carvalho F 《Free radical biology & medicine》2006,41(8):1213-1224
The widespread use of the nonselective contact herbicide paraquat (PQ) has been the cause of thousands of deaths from both accidental and voluntary ingestion. The main target organ for PQ toxicity is the lung. No antidote or effective treatment to decrease PQ accumulation in the lung or to disrupt its toxicity has yet been developed. The present study describes a procedure that leads to a remarkable decrease in PQ accumulation in the lung, together with an increase in its fecal excretion and a subsequent decrease in several biochemical and histopathological biomarkers of toxicity. The administration of dexamethasone (100 mg/kg ip) to Wistar rats, 2 h after PQ intoxication (25 mg/kg ip), decreased the lung PQ accumulation to about 40% of the group exposed to only PQ and led to an improvement in tissue healing in just 24 h as a result of the induction of de novo synthesis of P-glycoprotein (P-gp). The involvement of P-gp in these effects was confirmed by Western blot analysis and by the use of a competitive inhibitor of this transporter, verapamil (10 mg/kg ip), which, given 1 h before dexamethasone, blocked its protective effects, causing instead an increase in lung PQ concentration and an aggravation of toxicity. In conclusion, the induction of P-gp, leading to a decrease in lung levels of PQ and the consequent prevention of toxicity, seems to be a new and promising treatment for PQ poisonings that should be further clinically tested. 相似文献
54.
Cerezo-Guisado MI García-Román N García-Marín LJ Alvarez-Barrientos A Bragado MJ Lorenzo MJ 《The Biochemical journal》2007,406(1):175-183
Arachidonic acid is a potential paracrine agent released by the uterine endometrial epithelium to induce PTGS2 [PG (prostaglandin)-endoperoxide synthase 2] in the stroma. In the present study, bovine endometrial stromal cells were used to determine whether PTGS2 is induced by arachidonic acid in stromal cells, and to investigate the potential role of PPARs (peroxisome-proliferator-activated receptors) in this effect. Arachidonic acid increased PTGS2 levels up to 7.5-fold within 6 h. The cells expressed PPARalpha and PPARdelta (also known as PPARbeta) (but not PPARgamma). PTGS2 protein level was increased by PPAR agonists, including polyunsaturated fatty acids, synthetic PPAR ligands, PGA1 and NSAIDs (non-steroidal anti-inflammatory drugs) with a time course resembling that of arachidonic acid. Use of agonists and antagonists indicated PPARalpha (but not PPARdelta or PPARgamma) was responsible for PTGS2 induction. PTGS2 induction by arachidonic acid did not require PG synthesis. PTGS2 levels were increased by the PKC (protein kinase C) activators 4beta-PMA and PGF(2alpha), and the effects of arachidonic acid, NSAIDs, synthetic PPAR ligands and 4beta-PMA were blocked by PKC inhibitors. This is consistent with PPAR phosphorylation by PKC. Induction of PTGS2 protein by 4beta-PMA in the absence of a PPAR ligand was decreased by the NF-kappaB (nuclear factor kappaB) inhibitors MG132 and parthenolide, suggesting that PKC acted through NF-kappaB in addition to PPAR phosphorylation. Use of NF-kappaB inhibitors allowed the action of arachidonic acid as a PPAR agonist to be dissociated from an effect through PKC. The results are consistent with the hypothesis that arachidonic acid acts via PPARalpha to increase PTGS2 levels in bovine endometrial stromal cells. 相似文献
55.
Plant and Soil - Southern South American Proteaceae can occupy soils that are rich in total phosphorus (P) but poor in available P (for example volcanic soils) thanks to their cluster roots (CR),... 相似文献
56.
水分和磷调控的澳大利亚桉树林林下植物群落组合对二氧化碳浓度升高的响应
鉴于林下植物群落具有的关键性功能作用和全球范围内巨大的森林覆盖面积,研究林下群落对 CO2浓度升高(eCO2)的响应以及土壤资源在这些响应中的作用,对于了解CO2浓度升高对森林生态系统造成的影响非常重要。本研究评估了在澳大利亚东部磷有限的桉树林林下群落中,两种限制性的资源(即水分和磷)在发芽、物候、覆盖率、群落组成和叶片性状等方面对eCO2响应的作用。我们收集了含有当地土壤种子库的土壤,在温室条件下种植实验性的林下植物群落。研究结果表明,添加磷提高了植物的总体覆盖率,特别是在生长期的最初4 周以及水分含量高的条件下,而且该响应是由植物群落中的类禾本科植物所驱动。然而,随着实验的进行,不同处理方法之间的差异逐渐减小,所有处理在大约11周后均达到了80%左右的植物覆盖率。相反,植物覆盖率并未受到eCO2 的影响。多元分析结果反映出植物群落组成随时间的变化,盆栽从以裸土为主变为以高覆盖率的多样化群落为主。但是在实验过程中,磷的添加以及水分可利用性和CO2之间的相互作用都对植物群落随时间的变化轨迹有所影响。CO2浓度的升高也增加了群落水平的比叶面积,这表明植物群落对eCO2的功能适应可能发生在成分响应开始之前。鉴于我们用种子库培育的林下群落对eCO2 的响应随着时间的推移而有 所变化,并且受到与磷和水分可利用性的相互作用的调节。我们的结果表明,在水分含量有限的系统中, 特别是在土壤养分可利用性低所导致的生产力响应受限的情况下,CO2浓度的升高在塑造植物群落方面作用有限。 相似文献
57.
María Capa João Miguel de Matos Nogueira Maíra Cappellani Silva Rossi 《Journal of morphology》2011,272(3):302-319
Fan worms (Sabellidae) possess paired modified prostomial structures at the base of the radiolar crown, dorso‐lateral to the mouth, called dorsal lips. The dorsal lips are involved in the sorting of particles collected by the radiolar crown. The range of variation in the morphology of dorsal lips is extensive, and probably this is not only due to adaptations to different environments and feeding preferences but also due to phylogenetic constraints. In this study, we describe and compare the morphology of dorsal lips in a range of sabellid taxa based on histological cross‐sections of these structures, and compare our data and terminology with those of previous studies. Dorsal lips are maintained erect in most taxa by a modified radiole fused to them known as dorsal radiolar appendage. We suggest that dorsal radiolar appendages with an internal supporting axis (cellular or acellular) and probably also the ventral lips are synapomorphies of the family. J. Morphol., 2011. © 2010 Wiley‐Liss, Inc. 相似文献
58.
Contreras MA Haq E Uto T Singh I Singh AK 《Archives of biochemistry and biophysics》2008,477(2):211-218
Krabbe disease is a neuroinflammatory disorder in which galactosylsphingosine (psychosine) accumulates in nervous tissue. To gain insight into whether the psychosine-induced effects in nervous tissue extend to peripheral organs, we investigated the expression of cytokines and their effects on peroxisomal structure/functions in twitcher mouse liver (animal model of Krabbe disease). Immunofluorescence analysis demonstrated TNF-α and IL-6 expression, which was confirmed by mRNAs quantitation. Despite the presence of TNF-α, lipidomic analysis did not indicate a significant decrease in sphingomyelin or an increase in ceramide fractions. Ultrastructural analysis of catalase-dependent staining of liver sections showed reduced reactivity without significant changes in peroxisomal contents. This observation was confirmed by assaying catalase activity and quantitation of its mRNA, both of which were found significantly decreased in twitcher mouse liver. Western blot analysis demonstrated a generalized reduction of peroxisomal matrix and membrane proteins. These observations indicate that twitcher mouse pathobiology extends to the liver, where psychosine-induced TNF-α and IL-6 compromise peroxisomal structure and functions. 相似文献
59.
Eira Bermúdez-Cuamatzin Alejandro A. Ríos-Chelén Diego Gil Constantino Macías Garcia 《Biology letters》2011,7(1):36-38
Research has shown that bird songs are modified in different ways to deal with urban noise and promote signal transmission through noisy environments. Urban noise is composed of low frequencies, thus the observation that songs have a higher minimum frequency in noisy places suggests this is a way of avoiding noise masking. Most studies are correlative and there is as yet little experimental evidence that this is a short-term mechanism owing to individual plasticity. Here we experimentally test if house finches (Carpodacus mexicanus) can modulate the minimum frequency of their songs in response to different noise levels. We exposed singing males to three continuous treatments: low–high–low noise levels. We found a significant increase in minimum frequency from low to high and a decrement from high to low treatments. We also found that this was mostly achieved by modifying the frequency of the same low-frequency syllable types used in the different treatments. When different low-frequency syllables were used, those sung during the noisy condition were longer than the ones sang during the quiet condition. We conclude that house finches modify their songs in several ways in response to urban noise, thus providing evidence of a short-term acoustic adaptation. 相似文献
60.