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91.
The conditioned medium from osteo‐differentiating human mesenchymal stem cells affects the viability of triple negative MDA‐MB231 breast cancer cells
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This study aimed to investigate the effect of conditioned media (CM) from osteo‐differentiating and adipo‐differentiating human mesenchymal stem cells (MSCs) isolated from lipoaspirates of healthy female donors on the viability of triple‐negative breast cancer cells MDA‐MB231. The CM of undifferentiated and differentiating MSCs were collected after 7, 14, 21 and 28 days of culture. The effects of MSC CM on cell proliferation were assessed using an 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay after 24 h. The effects of osteo‐differentiating cell CM on apoptotic promotion, cell cycle impairment, mitochondrial transmembrane potential dissipation, production of reactive oxygen species and autophagosome accumulation were analysed by flow cytometry and Western blot. MTT assay showed that only CM collected from osteo‐induced cells at day 28 (d28O‐CM) reduced tumour cell viability. Treatment with d28O‐CM restrained cell cycle progression through G2 phase, elicited a caspase‐8‐driven apoptotic effect already after 5 h of culture, and down‐regulated autophagosome accumulation and beclin‐1 expression. The finding that factor(s) secreted by osteo‐differentiating MSCs shows properties of an apoptotic inducer and autophagy inhibitor on triple‐negative breast cancer cells may have an important applicative potential that deserves further investigation. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
92.
J G Vostal A B Mukherjee L Miele N R Shulman 《Biochemical and biophysical research communications》1989,165(1):27-36
The active site for uteroglobin inhibition of phospholipase A2 has been localized to a nonapeptide (P1) which is partially homologous to a nonapeptide (P2) in lipocortin, which also inhibits phospholipase A2. P1 and P2 share an identical tetrapeptide (P4) which is required for inhibition, although P4 alone does not inhibit this enzyme. We found the mechanism of inhibition of platelet aggregation and secretion by the nonapeptides and P4 varied depending on whether platelets were thrombin- or ADP-activated. All three peptides decrease thrombin esterolytic activity and thereby inhibit thrombin-induced platelet activation. P1 decreases ADP-induced aggregation and serotonin secretion by inhibiting phospholipase A2 whereas P4 decreases only aggregation by blocking fibrinogen binding to activated platelets. The P4 sequence in P1 may affect the interaction of P1 with platelets since the presence of P4 potentiates P1 inhibition of platelet activation. 相似文献
93.
T. Limongi A. Rocchi F. Cesca H. Tan E. Miele A. Giugni M. Orlando M. Perrone Donnorso G. Perozziello Fabio Benfenati Enzo Di Fabrizio 《Molecular neurobiology》2018,55(12):8788-8798
Biopolymers are increasingly employed for neuroscience applications as scaffolds to drive and promote neural regrowth, thanks to their ability to mediate the upload and subsequent release of active molecules and drugs. Synthetic degradable polymers are characterized by different responses ranging from tunable distension or shrinkage to total dissolution, depending on the function they are designed for. In this paper we present a biocompatible microfabricated poly-ε-caprolactone (PCL) scaffold for primary neuron growth and maturation that has been optimized for the in vitro controlled release of brain-derived neurotrophic factor (BDNF). We demonstrate that the designed morphology confers to these devices an enhanced drug delivery capability with respect to monolithic unstructured supports. After incubation with BDNF, micropillared PCL devices progressively release the neurotrophin over 21 days in vitro. Moreover, the bioactivity of released BDNF is confirmed using primary neuronal cultures, where it mediates a consistent activation of BDNF signaling cascades, increased synaptic density, and neuronal survival. These results provide the proof-of-principle on the fabrication process of micropatterned PCL devices, which represent a promising therapeutic option to enhance neuronal regeneration after lesion and for neural tissue engineering and prosthetics. 相似文献
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Serum From Advanced Heart Failure Patients Promotes Angiogenic Sprouting and Affects the Notch Pathway in Human Endothelial Cells
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Micaela Pannella Cristiana Caliceti Francesca Fortini Giorgio Aquila Francesco Vieceli Dalla Sega Antonio Pannuti Cinzia Fortini Marco Bruno Morelli Alessandro Fucili Gloria Francolini Rebecca Voltan Paola Secchiero Giovanni Dinelli Emanuela Leoncini Manuela Ferracin Silvana Hrelia Lucio Miele Paola Rizzo 《Journal of cellular physiology》2016,231(12):2700-2710
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Zhou Qinwei Desai Smruti A. Wang Xinhui Noronha Elvyra J. Neri Mariangela Ferrone Soldano 《International journal of peptide research and therapeutics》1999,6(1):77-86
Summary Utilizing phage display peptide libraries, we have identified and mapped the antigenic determinants recognized by mouse monoclonal
antibodies (mAb) on two sets of immunologically important molecules, HLA class I and class II antigens. Anti-HLA class I mAb
TP25.99 recognizes a conformational and a linear determinant on distinct regions of the HLA class I α3 domain. Anti-HLA class
I mAb HO-4 recognizes a conformational determinant on the α2 domain of HLA-A2 and A28 allospecificities. Anti-HLA-DR1,-DR4,-DR6,-DR8,-DR9
mAb SM/549 recognizes a conformational determinant on the β chain of HLA class II antigens. These results indicate the versatility
of phage display peptide libraries to characterize antigenic determinants recognized by anti-HLA mAb. 相似文献
100.
Turhan A Tsuda A Konerding MA Lin M Miele L Lee G Mentzer SJ 《In vitro cellular & developmental biology. Animal》2008,44(10):426-433
A central feature of intussusceptive angiogenesis is the development of an intravascular pillar that bridges the opposing
sides of the microvessel lumen. In this report, we created polydimethyl siloxane (PDMS) microchannels with geometric proportions
based on corrosion casts of the colon microcirculation. The structure of the PDMS microchannels was a bifurcated channel with
an intraluminal pillar in the geometric center of the bifurcation. The effect of the intraluminal pillar on particle flow
paths was investigated using an in vitro perfusion system. The microchannels were perfused with fluorescent particles, and
the particle movements were recorded using fluorescence videomicroscopy. We found that the presence of an intravascular pillar
significantly decreased particle velocity in the bifurcation system (p < 0.05). In addition, the pillar altered the trajectory of particles in the center line of the flow stream. The particle
trajectory resulted in prolonged pillar contact as well as increased residence time within the bifurcation system (p < 0.001). Our results suggest that the intravascular pillar not only provides a mechanism of increasing resistance to blood
flow but may also participate in spatial redistribution of cells within the flow stream.
Supported in part by NIH Grants HL47078, HL75426, HL054885, HL070542 and HLO74022. 相似文献