Using the product environmental footprint for supply chain management: lessons learned from a case study on pork

Purpose

The purpose of this study was to test the chain-organization environmental footprint (chain-OEF) approach by applying it to part of a pork production chain in Belgium. The approach is supposed to provide insight into the environmental impact of a specific production chain in an efficient manner by applying pragmatic data collection throughout the chain. This is achieved by allocating the environmental impact of each of the production sites to the product of interest using straightforward allocation rules.

Methods

The cradle-to-gate (up to retail) environmental impact of pork was determined by life cycle assessment (LCA), in line with the product and organisation environmental footprint guidelines (PEF and OEF; European Commission 2013b). Foreground data was gathered at a feed production site, two farmers, a slaughterhouse and a meat processing site. All foreground operations are part of the same pork production chain in Belgium. The chain was completed using background data from Ecoinvent v3.01 (Wernet et al. 2016), Agri-Footprint v1.0 (Blonk 2014), European Life Cycle Database v3.0, LCA Food Database (Nielsen et al. 2003) and OEF Sector Rules Retail (Humbert et al. 2015b). The impact was quantified using the international reference life cycle data system (ILCD) midpoint method for 14 impact categories, but focussing on climate change.

Results and discussion

The total carbon footprint of the cradle-to-gate pork production system equals 0.46 kg CO₂-eq. (100 g pork)^?1. This result is quite similar to that of earlier studies analysing the pork production chain: 0.58 and 0.57 kg CO₂-eq. (100 g pork)^?1 (Bracquené et al. 2011, Agri-Footprint 2014). Most of the carbon footprint was caused by feed production and more specifically, by the feed ingredients and their transport. Grains, soy and palm oil have the largest impact contributions. The farms are responsible for most of the remaining impact. N₂O and CH₄ emissions are the largest cause of greenhouse gas emissions at the farms. Also, in the other 13 considered impact categories, feed production and farming are responsible for more than half of the total impact, mostly followed by meat processing.

Conclusions

Applying the chain-OEF approach in this study has shown that a chain LCA can be performed successfully and pragmatic data collection allows obtaining LCA results relatively fast, especially for small or medium-sized enterprises (SMEs). Whereas data availability was not such an issue, the main bottlenecks identified are data management and the link of LCA to other disciplines such as engineering, policy, etc. which could increase the added value of LCA studies.

     

Islamic gatherings: experiences of discrimination and religious affirmation across established and new immigrant communities

To what extent are perceptions of discrimination associated with religious affirmation among Muslim minorities in the Netherlands? Drawing on recent nationally representative surveys among self-identified Muslims from five ethnic groups in the Netherlands, we test boundary conditions of reactive religiosity. Our findings indicate that for Muslims from established immigrant groups, perceptions of discrimination are associated with more frequent religious attendance, but that this is not the case for Muslims from smaller, less established ethnic communities. Findings are interpreted using a boundary framework.     

Reconstructing the Rasch-Built Myotonic Dystrophy Type 1 Activity and Participation Scale

IntroductionA previously published Rasch-built activity and participation scale specifically designed for patients with myotonic dystrophy type 1 (DM1) was criticized for having been constructed in a relatively small cohort of patients and containing items too broadly phrased for DM1 patients, thus hampering its clinical use.MethodsWe report the results of the reconstructed Rasch-built DM1 activity and participation scale for clinical use (DM1-Activ^C) through Rasch analyses using an expanded questionnaire containing 146 more simply phrased activity and participation inquiries completed by 340 patients with DM1.ResultsThrough stepwise investigation including data quality control, model fit, response category ordering, local dependency and item bias, we succeeded in reconstructing the DM1-Activ^C consisting of 25 items that showed good Rasch model fit, including construct convergent validity, items’ weights and persons’ locations reliability, and unidimensionality.ConclusionThe DM1-Activ^C scale has been reconstructed and fulfills all modern clinimetric requirements. Its use is recommended in future longitudinal trials in patients with DM1 to determine its responsiveness.     

Identification of small heat shock protein B8 (HSP22) as a novel TLR4 ligand and potential involvement in the pathogenesis of rheumatoid arthritis

Dendritic cells (DCs) are specialized APCs that can be activated upon pathogen recognition as well as recognition of endogenous ligands, which are released during inflammation and cell stress. The recognition of exogenous and endogenous ligands depends on TLRs, which are abundantly expressed in synovial tissue from rheumatoid arthritis (RA) patients. Furthermore TLR ligands are found to be present in RA serum and synovial fluid and are significantly increased, compared with serum and synovial fluid from healthy volunteers and patients with systemic sclerosis and systemic lupus erythematosus. Identification of novel endogenous TLR ligands might contribute to the elucidation of the role of TLRs in RA and other autoimmune diseases. In this study, we investigated whether five members of the small heat shock protein (HSP) family were involved in TLR4-mediated DC activation and whether these small HSPs were present in RA synovial tissue. In vitro, monocyte-derived DCs were stimulated with recombinant alphaA crystallin, alphaB crystallin, HSP20, HSPB8, and HSP27. Using flow cytometry and multiplex cytokine assays, we showed that both alphaA crystallin and HSPB8 were able to activate DCs and that this activation was TLR4 dependent. Furthermore, Western blot and immunohistochemistry showed that HSPB8 was abundantly expressed in synovial tissue from patients with RA. With these experiments, we identified sHSP alphaA crystallin and HSPB8 as two new endogenous TLR4 ligands from which HSPB8 is abundantly expressed in RA synovial tissue. These findings suggest a role for HSPB8 during the inflammatory process in autoimmune diseases such as RA.     

Thiophene-containing dinucleating ligands for the copper-catalyzed oxidative coupling of 2,6-dimethylphenol

The copper-catalysed polymerisation of 2,6-dimethylphenol leads to poly(2,6-dimethyl-1,4-phenylene) ether, which is a widely used high-performance thermoplastic. The oxidative coupling reactions have been performed in acetonitrile with structurally related dinucleating phenol-based “end-off” compartmental N,O,S- ligands. The bio-inspired copper catalysts appeared to be of great interest for a better understanding of the parameters governing the catalytic activities. Steric effects (difference between three side-arm and four side-arm ligands), as well as electronic effects (thiophene versus pyridine substituents) have been evidenced on the initial dioxygen-uptake rates.     

FAF1, a gene that is disrupted in cleft palate and has conserved function in zebrafish

Cranial neural crest (CNC) is a multipotent migratory cell population that gives rise to most of the craniofacial bones. An intricate network mediates CNC formation, epithelial-mesenchymal transition, migration along distinct paths, and differentiation. Errors in these processes lead to craniofacial abnormalities, including cleft lip and palate. Clefts are the most common congenital craniofacial defects. Patients have complications with feeding, speech, hearing, and dental and psychological development. Affected by both genetic predisposition and environmental factors, the complex etiology of clefts remains largely unknown. Here we show that Fas-associated factor-1 (FAF1) is disrupted and that its expression is decreased in a Pierre Robin family with an inherited translocation. Furthermore, the locus is strongly associated with cleft palate and shows an increased relative risk. Expression studies show that faf1 is highly expressed in zebrafish cartilages during embryogenesis. Knockdown of zebrafish faf1 leads to pharyngeal cartilage defects and jaw abnormality as a result of a failure of CNC to differentiate into and express cartilage-specific markers, such as sox9a and col2a1. Administration of faf1 mRNA rescues this phenotype. Our findings therefore identify FAF1 as a regulator of CNC differentiation and show that it predisposes humans to cleft palate and is necessary for lower jaw development in zebrafish.     

Inactivation of murine norovirus 1, coliphage phiX174, and Bacteroides [corrected] fragilis phage B40-8 on surfaces and fresh-cut iceberg lettuce by hydrogen peroxide and UV light

In this study, the inactivating properties of liquid hydrogen peroxide (L-H(2)O(2)), vaporized hydrogen peroxide (V-H(2)O(2)), UV light, and a combination of V-H(2)O(2) and UV light were tested on murine norovirus 1 (MNV-1) and bacteriophages (φX174 and B40-8) as models for human noroviruses. Disinfection of surfaces was examined on stainless steel discs based on European Standard EN 13697 (2001). For fresh-produce decontamination, a mixture of the viruses was inoculated onto shredded iceberg lettuce and treated after overnight incubation at 2°C. According to our results, L-H(2)O(2) (2.1%) was able to inactivate MNV-1 and φX174 on stainless steel discs by approximately 4 log(10) units within 10 min of exposure, whereas for B40-8, 15% of L-H(2)O(2) was needed to obtain a similar reduction in 10 min. Only a marginal reduction (≤1 log(10) unit after 5 min of exposure) by V-H(2)O(2) (2.52%) was achieved for the tested model viruses, although in combination with UV light, a 4-log(10)-unit decrease within 5 min of treatment was observed on stainless steel discs. Similar trends were observed for the decontamination of shredded iceberg lettuce, but the viral decline was reduced. These results demonstrated that both L-H(2)O(2) and a combination of V-H(2)O(2) and UV light can be used for norovirus inactivation on surfaces; V-H(2)O(2) (2.52%) in combination with UV light is promising for decontamination of fresh produce with much less consumption of water and disinfectant.     

Transplacental Transmission of Bluetongue Virus Serotype 1 and Serotype 8 in Sheep: Virological and Pathological Findings

The Bluetongue virus serotype 8 (BTV-8) strain, which emerged in Europe in 2006, had an unusually high ability to cause foetal infection in pregnant ruminants. Other serotypes of BTV had already been present in Europe for more than a decade, but transplacental transmission of these strains had never been demonstrated. To determine whether transplacental transmission is a unique feature of BTV-8 we compared the incidence and pathological consequences of transplacental transmission of BTV-8 to that of BTV-1. Nine pregnant ewes were infected with either BTV-8 or BTV-1. The BTV strains used for the infection were field strains isolated on embryonated chicken eggs and passaged twice on mammalian cells. Blood samples were taken to monitor the viraemia in the ewes. Four weeks after the infection, the foetuses were examined for pathological changes and for the presence of BTV. BTV-8 could be demonstrated in 12 foetuses (43%) from 5 ewes (56%). %). BTV-1 was detected in 14 foetuses (82%) from 6 ewes (67%). Pathological changes were mainly found in the central nervous system. In the BTV-8 group, lympho-histiocytic infiltrates, gliosis and slight vacuolation of the neuropil were found. BTV-1infection induced a severe necrotizing encephalopathy and severe meningitis, with macroscopic hydranencephaly or porencephaly in 8 foetuses. In our experimental setting, using low passaged virus strains, BTV-1 was able to induce transplacental transmission to a higher incidence compared to BTV-8, causing more severe pathology.     

Classification of Forefoot Plantar Pressure Distribution in Persons with Diabetes: A Novel Perspective for the Mechanical Management of Diabetic Foot?

Background

The aim of this study was to identify groups of subjects with similar patterns of forefoot loading and verify if specific groups of patients with diabetes could be isolated from non-diabetics.

Methodology/Principal Findings

Ninety-seven patients with diabetes and 33 control participants between 45 and 70 years were prospectively recruited in two Belgian Diabetic Foot Clinics. Barefoot plantar pressure measurements were recorded and subsequently analysed using a semi-automatic total mapping technique. Kmeans cluster analysis was applied on relative regional impulses of six forefoot segments in order to pursue a classification for the control group separately, the diabetic group separately and both groups together. Cluster analysis led to identification of three distinct groups when considering only the control group. For the diabetic group, and the computation considering both groups together, four distinct groups were isolated. Compared to the cluster analysis of the control group an additional forefoot loading pattern was identified. This group comprised diabetic feet only. The relevance of the reported clusters was supported by ANOVA statistics indicating significant differences between different regions of interest and different clusters.

Conclusion/s Significance

There seems to emerge a new era in diabetic foot medicine which embraces the classification of diabetic patients according to their biomechanical profile. Classification of the plantar pressure distribution has the potential to provide a means to determine mechanical interventions for the prevention and/or treatment of the diabetic foot.