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Malgorzata Kotulska Joanna Basalyga Maria B. Derylo Przemyslaw Sadowski 《The Journal of membrane biology》2010,236(1):37-41
Single metastable nanopores, appearing before the actual electroporation under constant-current conditions, are used to characterize
the onset of electroporation. Unlike the long-lived electropores typical of the current controlled methods, these pores survive
for milliseconds and observing them is possible due to slow development of electroporation, provided by the gradual accumulation
of charges on a planar membrane. Analysis of the metastable pore appearance frequency and lifetime shows the first introductory
stage of electroporation. During this stage two species of metastable pores open, the majority of very low conductance that
seem not fully developed as hydrophilic electropores. The experiments reveal that voltage value defines the electroporation
onset while the current value affects the rate of electroporation. Membrane capacitance has a great impact on the membrane
susceptibility to the pore appearance, related to its thickness and integrity. Pores of nonperfect membranes appear more easily,
but they do not live any longer than others. 相似文献
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Mieczyslawa Derylo Maria Glowacka Anna Skorupska Zbigniew Lorkiewicz 《Archives of microbiology》1981,130(4):322-324
Lignobacter strain K17 is able to degrade aromatic compounds and to fix atmospheric nitrogen. It was proved that capacity for nitrogen fixation by Lignobacter was plasmid mediated. Plasmid pUCS100 (17.5 Mdal) carrying nif genes was transferred from Lignobacter to Escherichia coli SK1592 and Salmonella typhimurium. The transposon Tn9 was translocated to pUCS100 to facilitate selection of Nif+ bacteria. E. coli SK1592 harboring the new plasmid (pUCS101) reduced acetylene under anaerobic conditions. Plasmids pUCS100 and pUCS101 were not stably maintained in E. coli and S. typhimurium.Abbreviations Mdal
megadalton
- CsCl-EtBr
caesium chloride ethidium bromide
- m.o.i.
multiplicity of infection 相似文献
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