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121.
We investigated patterns of acorn growth in Quercus variabilis Blume and Quercus serrata Thunb., seasonal trends in emergence of seed insects found in the acorns, oviposition periods of the insects and falling periods of insect-infested acorns. In Q. variabilis, two insect guilds were associated with acorn development: (i) the immature acorn feeding (IAF) guild [Curculionidae sp., Poecilips cardamomi (Schaufuss), and Characoma ruficirra (Hampson)]; and (ii) the mature acorn feeding (MAF) guild [Curculio robustus (Roelofs), Curculio sikkimensis (Heller), and Cydia glandicolana (Danilevsky)]. In Q. serrata, there were three guilds: (i) the pistillate flower feeding (PFF) guild (cynipid wasp); (ii) the IAF guild [sap absorption by Mechoris ursulus (Roelofs)]; and (iii) the MAF guild (M. ursulus, C. sikkimensis, Cydia danilevskyi (Kuznetzov), C. glandicolana and Autostichidae sp.). The succession of guilds during acorn development may be a consequence of the use by different species of the limited food resource. The lack of a PFF guild in Q. variabilis that was found in our field site would have a positive effect on IAF guilds in utilizing the acorns. 相似文献
122.
Derren J. Heyes Basile Khara Michiyo Sakuma Samantha J. O. Hardman Ronan O'Cualain Stephen E. J. Rigby Nigel S. Scrutton 《PloS one》2012,7(12)
Phytochromes are dimeric photoreceptors that regulate a range of responses in plants and microorganisms through interconversion of red light-absorbing (Pr) and far-red light-absorbing (Pfr) states. Photoconversion between these states is initiated by light-driven isomerization of a bilin cofactor, which triggers protein structural change. The extent of this change, and how light-driven structural changes in the N-terminal photosensory region are transmitted to the C-terminal regulatory domain to initiate the signalling cascade, is unknown. We have used pulsed electron-electron double resonance (PELDOR) spectroscopy to identify multiple structural transitions in a phytochrome from Synechocystis sp. PCC6803 (Cph1) by measuring distances between nitroxide labels introduced into the protein. We show that monomers in the Cph1 dimer are aligned in a parallel ‘head-to-head’ arrangement and that photoconversion between the Pr and Pfr forms involves conformational change in both the N- and C-terminal domains of the protein. Cryo-trapping and kinetic measurements were used to probe the extent and temporal properties of protein motions for individual steps during photoconversion of Cph1. Formation of the primary photoproduct Lumi-R is not affected by changes in solvent viscosity and dielectric constant. Lumi-R formation occurs at cryogenic temperatures, consistent with their being no major structural reorganization of Cph1 during primary photoproduct formation. All remaining steps in the formation of the Pfr state are affected by solvent viscosity and dielectric constant and occur only at elevated temperatures, implying involvement of a series of long-range solvent-coupled conformational changes in Cph1. We show that signalling is achieved through ultrafast photoisomerization where localized structural change in the GAF domain is transmitted and amplified to cause larger-scale and slower conformational change in the PHY and histidine kinase domains. This hierarchy of timescales and extent of structural change orientates the histidine kinase domain to elicit the desired light-activated biological response. 相似文献
123.
124.
Preservation of Purified Peanut Stripe and Turnip Mosaic Potyviruses by Freezing and Freeze-drying 总被引:1,自引:0,他引:1
During storage at - 20 °C of purified preparations of peanut stripe and turnip mosaic potyviruses, infectivity of intact particles and RNA extracted from preserved preparations decreased. This decrease can be attributed to several factors such as virus aggregation and alterations of the nucleic acids and coat proteins. However, the addition to the preparations of 0.5% peptone or 1% sucrose permitted a high level of infectivity to be retained for extended periods. Freeze-drying led to aggregation of the particles and in a marked decrease of infectivity. Virus aggregatioti and RNA degradation were suppressed by the addition of protectants such as 0.5% lysine. Results suggest that other potyviruses may be well preserved under similar conditions. 相似文献
125.
Kiyozo Hasegawa Michiyo Murata Tetsuya Suzuki Akihiro Takigawa 《Bioscience, biotechnology, and biochemistry》2013,77(9):2167-2174
The activities of phospholipids acyl-hydrolases in an enzyme preparation from a mold, Corticium centrifugum, were examined. Lecithin acyl-hydrolase had an optimal pH at 3.5. The reaction proceeded beyond the range of 50%. Sigmoidal curves observed suggested the presence of lysophospholipase in the preparation. The latter enzyme activity was found to be seven times as strong as the former at the same pH. Fractionation by DEAE-Sephadex chromatography and analysis of the reaction products demonstrated that the main component of lecithin acyl-hydrolase was phospholipase B, which hydrolyzed both of fatty acyl ester groups of lecithin. This activity was found to be present as a separate enzyme from most of lysophospholipase. 相似文献
126.
Mitsunori Kono Tsuneo Oda Michiko Tawada Takashi Imada Yoshihiro Banno Naohiro Taya Tetsuji Kawamoto Hidekazu Tokuhara Yoshihide Tomata Naoki Ishii Atsuko Ochida Yoshiyuki Fukase Tomoya Yukawa Shoji Fukumoto Hiroyuki Watanabe Keiko Uga Akira Shibata Hideyuki Nakagawa Satoshi Yamamoto 《Bioorganic & medicinal chemistry》2018,26(2):470-482
A series of tetrahydroisoquinoline derivatives were designed, synthesized, and evaluated for their potential as novel orally efficacious retinoic acid receptor-related orphan receptor-gamma t (RORγt) inverse agonists for the treatment of Th17-driven autoimmune diseases. We carried out cyclization of the phenylglycinamide core by structure-based drug design and successfully identified a tetrahydroisoquinoline carboxylic acid derivative 14 with good biochemical binding and cellular reporter activity. Interestingly, the combination of a carboxylic acid tether and a central fused bicyclic ring was crucial for optimizing PK properties, and the compound 14 showed significantly improved PK profile. Successive optimization of the carboxylate tether led to the discovery of compound 15 with increased inverse agonistic activity and an excellent PK profile. Oral treatment of mice with compound 15 robustly and dose-dependently inhibited IL-17A production in an IL23-induced gene expression assay. 相似文献
127.
128.
Yutaka Tanaka Masato Sasaki Fumie Ito Toshio Aoyama Michiyo Sato-Okamoto Azusa Takahashi-Nakaguchi Hiroji Chibana Nobuyuki Shibata 《Fungal biology》2018,122(1):19-33
Candida glabrata is the second most common source of Candida infections in humans. In this pathogen, the maintenance of cell wall integrity (CWI) frequently precludes effective pharmacological treatment by antifungal agents. In numerous fungi, cell wall modulation is reported to be controlled by endoplasmic reticulum (ER) stress, but how the latter affects CWI maintenance in C. glabrata is not clearly understood. Here, we characterized a C. glabrata strain harboring a mutation in the CNE1 gene, which encodes a molecular chaperone associated with nascent glycoprotein maturation in the ER. Disruption of cne1 induced ER stress and caused changes in the normal cell wall structure, specifically a reduction in the β-1,6-glucan content and accumulation of chitin. Conversely, a treatment with the typical ER stress inducer tunicamycin up-regulated the production of cell wall chitin but did not affect β-1,6-glucan content. Our results also indicated that C. glabrata features a uniquely evolved ER stress-mediated CWI pathway, which differs from that in the closely related species Saccharomyces cerevisiae. Furthermore, we demonstrated that ER stress-mediated CWI pathway in C. glabrata is also induced by the disruption of other genes encoding proteins that function in a correlated manner in the quality control of N-linked glycoproteins in the ER. These results suggest that calcineurin and ER quality control system act as a platform for maintaining CWI in C. glabrata. 相似文献
129.
Efficient transformation of pBR322 and its derived
plasmids, which have been widely used as cloning vectors in Escherichia
coli, was observed in Pseudomonas avenae (K1), the pathogen of
leaf blight disease in cereals. Moreover, there was a 10- to 50-fold
transformation efficiency (1.3–3.0 × 106/μg DNA) in the
proline-auxotrophic mutant (Pr47), whose virulence to rice seedlings
decreased. Similar enhancement of the frequency of transfer by mobilization
of RSF1010, a broad host range plasmid, was observed in the recipient Pr47
strain in mating with donor Pseudomonas syringae. The plasmids
harbored in these strains were maintained very stably after subcultures.
Thus, a highly efficient transformation system with pBR322-derived plasmids
used as a vector and Pseudomonas as a host bacterium was developed.
Received: 13 July 1996 / Accepted: 26 August 1996 相似文献
130.
A 3-Mb Sequence-Ready Contig Map Encompassing the Multiple Disease Gene Cluster on Chromosome 11q13.1-q13.3 总被引:1,自引:0,他引:1
Kitamura Eiko; Hosoda Fumie; Fukushima Michiyo; Asakawa Shuichi; Shimizu Nobuyoshi; Imai Takashi; Soeda Eiichi; Ohki Misao 《DNA research》1997,4(4):281-289
Despite the presence of several human disease genes on chromosome11q13, few of them have been molecularly cloned. Here, we reportthe construction of a contig map encompassing 11q13.1q13.3using bacteriophage P1 (P1), bacterial artificial chromosome(BAC), and P1-derived artificial chromosome (PAC). The contigmap comprises 32 P1 clones, 27 BAC clones, 6 PAC clones, and1 YAC clone and spans a 3-Mb region from D11S480 to D11S913.The map encompasses all the candidate loci of Bardet-Biedlesyndrome type I (BBS1) and spinocerebellar ataxia type 5 (SCA5),one-third of the distal region for hereditary paraganglioma2 (PGL2), and one-third of the central region for insulin-dependentdiabetes mellitus 4 (IDDM4). In the process of map construction,61 new sequence-tagged site (STS) markers were developed fromthe Not I linking clones and the termini of clone inserts. Wehave also mapped 30 ESTs on this map. This contig map will facilitatethe isolation of polymorphic markers for a more re.ned analysisof the disease gene region and identi.cation of candidate genesby direct cDNA selection, as well as prediction of gene functionfrom sequence information of these bacterial clones. 相似文献