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排序方式: 共有105条查询结果,搜索用时 9 毫秒
31.
Sakamoto J Morita S Oba K Matsui T Kobayashi M Nakazato H Ohashi Y;Meta-Analysis Group of the Japanese Society for Cancer of the Colon Rectum 《Cancer immunology, immunotherapy : CII》2006,55(4):404-411
The benefits of immunochemotherapy employing the biological response modifier polysaccharide K (PSK) for patients with curatively
resected colorectal cancer was reassessed by means of a meta-analysis of data with center randomization from 1,094 patients
enrolled in three clinical trials. In all three trials, patients were followed up for at least 5 years after surgery and enrollment
of the last patient and outcomes for standard chemotherapy were compared with those for chemotherapy plus PSK. The endpoints
were overall survival and disease-free survival; and intent-to-treat analysis was performed without patient exclusion. Data
were analyzed using the weighted average of the individual log hazard ratios. The overall survival risk ratio for all eligible
patients was 0.71 (95% confidence interval (CI) : 0.55–0.90; P=0.006), and the disease-free survival risk ratio was 0.72 (95% CI: 0.58–0.90; P=0.003). The results of this meta-analysis suggest that adjuvant immunochemotherapy with PSK can improve both survival and
disease-free survival of patients with curatively resected colorectal cancer. 相似文献
32.
Isolation and characterization of SSR sequences from the genome and TAC clones of common wheat using the PCR technique. 总被引:1,自引:0,他引:1
We have developed the 2-step PCR method, a kind of suppression PCR procedure, to isolate simple sequence repeats (SSRs) from common wheat (Triticum aestivum L.) in a more convenient manner. This system requires neither genomic library screening nor the SSR-enrichment procedure. As a result, we designed 131 primer pairs based on isolated SSRs from not only genomic DNA, but also transformation-competent artificial chromosome (TAC) clones. It has been demonstrated that 34 of the 131 SSR markers developed were polymorphic among 8 wheat lines. Four of 34 polymorphic SSR markers were derived from TAC clones, indicating that this method could be applied to the targeted development of unique SSR markers in large genomic DNA libraries such as those composed of bacterial artificial chromosomes (BACs). A considerable number of isolated SSR clones had similarities with part of several long terminal repeats of retrotransposons (LTR-RTs) identified in various Triticeae genome sequences. Most of those SSRs showed smear amplification profiles, suggesting that a considerable number of dysfunctional SSRs originating from repetitive DNA components, especially LTR-RTs, might exist in the common wheat genome. 相似文献
33.
Apelin is a novel angiogenic factor in retinal endothelial cells 总被引:29,自引:0,他引:29
34.
The present study examined diurnal cycles of oocyte development and maturation in the kyusen wrasse, Halichoeres poecilopterus, and investigated the sensitivity of oocytes to maturation-inducing hormone (MIH) and gonadotropic hormone (GTH). Female fish were sampled at fixed intervals throughout the day, revealing that final oocyte maturation and ovulation were completed by 6:00 hr, and that spawning occurred daily between 6:00 and 9:00 hr. In vitro experiments showed that the steroids 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) and 17,20beta,21-trihydroxy-4-pregnen-3-one (20beta-S) were equally potent and highly effective inducers of germinal vesicle breakdown (GVBD) in kyusen wrasse oocytes. Additionally, circulating levels of 17,20beta-P and 20beta-S increased around the time of GVBD and ovulation, suggesting that 17,20beta-P and 20beta-S act as MIHs in the kyusen wrasse. Moreover, in vitro experiments clearly showed that kyusen wrasse oocytes had a daily developmental cycle of GTH and MIH sensitivity, and that oocytes that completed vitellogenesis acquired GTH-induced maturational competence. An endogenous GTH surge likely occurs between 12:00 and 15:00 hr, and this daily pre-maturational GTH surge probably controls the diurnal maturation cycles of kyusen wrasse oocytes. 相似文献
35.
Michiya Matsuyama Sumito Morita Takao Nasu Masaaki Kashiwagi 《Environmental Biology of Fishes》1998,52(1-3):281-290
The cycle of oocyte development of the bambooleaf wrasse, Pseudolabrus japonicus, was studied to elucidate the endocrinological mechanism of oocyte maturation in a marine teleost. A single female reared with two males spawned every day for 17 days in captivity, indicating that this species is a daily spawner. Ovarian histology revealed that germinal vesicle migration of the largest oocytes progressed from 12:00 to 3:00 h, and germinal vesicle breakdown (GVBD) was completed at 6:00 h. Ovulation and spawning occurred between 6:00 and 9:00 h. The effectiveness of human chorionic gonadotropin (HCG) and 17,20-dihydroxy-4-pregnen-3-one (17,20-P), which is one of the most potent steroidal inducers of GVBD in bambooleaf wrasse oocytes, in inducing final oocyte maturation was examined at eight different times of the day. The responsiveness of the oocyte to HCG and steroid differed at different times of the day. The GVBD could be induced by HCG but not 17,20-P at 9:00 h. Between 12:00 and 18:00 h, not only HCG but also 17,20-P induced GVBD. Both GVBD and ovulation spontaneously occurred between 0:00 and 6:00 h without any hormonal treatment. These results clearly showed that the oocyte of the bambooleaf wrasse possessed a diurnal maturation cycle. Responsiveness of oocytes to HCG appeared earlier than responsiveness to 17,20-P. This suggests that sensitivity to 17,20 -P is induced by gonadotropic hormone (GTH). 相似文献
36.
Mitsuo Masuno Shunji Tomatsu Yoshihiro Nakashima Toshinori Hori Seiji Fukuda Michiya Masue Kazuko Sukegawa Tadao Orii 《Genomics》1993,16(3)
Plasmid clones of three independent genomic fragments of the gene for human N -acetylgalactosamine-6-sulfate sulfatase (GALNS; EC 3.1.6.4) were utilized in a fluorescence in situ suppression hybridization study to assign the locus to chromosome 16q24. Enzyme assay for GALNS in a patient with del(16)(q22.1) confirmed this finding. 相似文献
37.
Kobayashi M Okamoto K Akimori T Tochika N Yoshimoto T Okabayashi T Sugimoto T Araki K 《Journal of molecular histology》2004,35(1):69-74
Thymidine phosphorylase (TP) is known to be more concentrated in human cancer tissues than in adjacent normal tissue based on findings using enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. However, the ultrastructural localization of TP in cancer tissues has not previously been demonstrated. We investigated the localization of TP in gastric cancer and colorectal cancer tissue by ELISA, immunohistochemistry, and immunoelectron microscopy. Between April 1997 and May 2000, we obtained surgically resected specimens from 42, 46, and 36 cases of advanced gastric, colon, and rectal cancer, respectively. ELISA demonstrated that the TP level was higher in cancer tissues than in adjacent normal tissue. Immunohistochemically, cancer cells were positive for the enzyme in some cases. However, in a number of cases immunopositive inflammatory cells were also present in cancerous tissues. At the electron microscope level, TP was diffusely distributed in the cytoplasm of cancer cells and in the mitochondria of the neutrophil in gastric cancer tissue. In rectal cancer tissues, cytoplasmic granules in macrophages in cancer tissues were immunoreactive for the TP. These findings suggest that TP is produced by macrophages and exists in neutrophils and cancer cells. 相似文献
38.
Takeshi Fujita Fumiko Chiwaki Ryou-u Takahashi Kazuhiko Aoyagi Kazuyoshi Yanagihara Takao Nishimura Masashi Tamaoki Masayuki Komatsu Rie Komatsuzaki Keisuke Matsusaki Hitoshi Ichikawa Hiromi Sakamoto Yasuhide Yamada Takeo Fukagawa Hitoshi Katai Hiroyuki Konno Takahiro Ochiya Teruhiko Yoshida Hiroki Sasaki 《PloS one》2015,10(6)
Diffuse-type solid tumors are often composed of a high proportion of rarely proliferating (i.e., dormant) cancer cells, strongly indicating the involvement of cancer stem cells (CSCs) Although diffuse-type gastric cancer (GC) patients have a poor prognosis due to high-frequent development of peritoneal dissemination (PD), it is limited knowledge that the PD-associated CSCs and efficacy of CSC-targeting therapy in diffuse-type GC. In this study, we established highly metastatic GC cell lines by in vivo selection designed for the enrichment of PD-associated GC cells. By microarray analysis, we found C-X-C chemokine receptor type 4 (CXCR4) can be a novel marker for highly metastatic CSCs, since CXCR4-positive cells can grow anchorage-independently, initiate tumors in mice, be resistant to cytotoxic drug, and produce differentiated daughter cells. In clinical samples, these CXCR4-positive cells were found from not only late metastasis stage (accumulated ascites) but also earlier stage (peritoneal washings). Moreover, treatment with transforming growth factor-β enhanced the anti-cancer effect of docetaxel via induction of cell differentiation/asymmetric cell division of the CXCR4-positive gastric CSCs even in a dormant state. Therefore, differentiation inducers hold promise for obtaining the maximum therapeutic outcome from currently available anti-cancer drugs through re-cycling of CSCs. 相似文献
39.
Kitano H Irie S Yamaguchi A Matsuyama M 《Journal of experimental zoology. Part A, Ecological genetics and physiology》2011,315(4):232-241
The diversity of reproductive strategies found in fish is attributed to the adaptation of gametogenesis to different habitats. To date, however, information about the dynamics of male gametogenesis and its molecular mechanisms of control by the brain-pituitary-gonadal (BPG) axis, a well-known facet of the endocrine system in vertebrates, is not sufficient to explain the variation in spawning and regulatory mechanisms among species. The bambooleaf wrasse (Pseudolabrus sieboldi) is a protogynous hermaphrodite fish that shows clear diurnal fluctuations in gonadotropin gene expression and serum sex steroid levels associated with spawning. In this study, morphometrical and histological analyses were performed to determine the number of spermatogonial generations in the testis of the sex-changed male (secondary testis). In addition, the diurnal dynamics of S-phase entry of germ cells was explored by measuring the frequency of BrdU-incorporating germ cells at different times of day. We found that the bambooleaf wrasse spermatozoa were generated through nine spermatogonial generations, followed by meiotic divisions accompanied by loss of some spermatocytes and spermatids through apoptosis. BrdU analyses revealed a high frequency of entry into S-phase of undifferentiated type A spermatogonia at 03:00?hr. On the other hand, the frequency of spermatocytes at S-phase of the cell cycle decreased during 00:00-09:00?hr. This study demonstrates for the first time the daily fluctuations of S-phase entry of male germ cells in fish. These results will provide a useful foundation for understanding the roles of endocrinological and cytological regulation of fish spermatogenesis. 相似文献
40.
Michiya Ohno Fumiko Deguchi Kumiko Izumi Hirotoshi Ishigaki Hiroshi Sarui Akihiko Sasaki Tomonori Segawa Takahiko Yamaki Takao Kojima Hiroshige Ohashi 《PloS one》2014,9(11)