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11.
Yoshiro Kinoshita Michinori Misaka Shuhei Kubota Hisao Ishikawa 《Bioscience, biotechnology, and biochemistry》2013,77(11):1975-1981
A mixture of two thionocarbamates was subjected to the acid-catalyzed rearrangement. A sample of the reaction mixture was analyzed by glpc and resolved into four components. From the cross-over result, it has been concluded that the acid-catalyzed rearrangement of alkyl thionocarbamates into the isomeric thiolcarbamates proceeds by an intermolecular alkylating mechanism. This conclusion was supported by the detection of a transalkylated intermediate. 相似文献
12.
Critical examinations were made on the conditions for preparing the sugar solutions to be analyzed by ion-exchange chromatography of sugar-borate complexes by the method of Khym and Zill. A procedure was proposed which gave the best recovery of sugars with minimum hydrolysis of sucrose. By means of this procedure, sugar solutions were prepared from potato tubers which had been stored at a high (30°C) or low temperature (6°C). Results of the chromatographic separation and determination of component sugars showed that main sugars present in potato tubers were sucrose, glucose, and fructose. Maltose and pentoses could not be detected. The contents of sucrose, glucose, and especially, fructose were far greater in potatoes stored at a low temperature than in those stored at a high temperature. 相似文献
13.
Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(1):52-58
Effects of materials extracted from potatoes with hot 80% ethanol on the course of phosphorylase reactions were examined. These materials had no effect on phosphorylase activities as determined by the rate of liberation of inorganic phosphate from glucose-1-phosphate in the presence of a large amount of primer; however, in the presence of a small amount of primer or in its absence, these materials had significant effects on the liberation of inorganic phosphate and starch formation. These results suggested the presence of a small amount of primers in these materials. Evidence was presented against the participation of d-enzyme on the formation of starch when partially purified preparations of potato phosphorylase or crude extracts from potatoes were used as the enzyme. 相似文献
14.
Hajime Taniguchi Fumihiko Odashima Makoto Igarashi Yoshiharu Maruyama Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(8):2107-2115
A bacterium which can utilize potato starch granules as sole carbon source was isolated and identified as Bacillus circulans from its physiological and biochemical properties. Scanning electron microscopic observation of potato starch granules recovered from the culture broth revealed that granules were degraded gradually from their surface resulting in elongated granules with layered structures on their surface. This bacterium produced extracellular amylase which can digest potato starch granules in vitro. The amylase has a unique property in that it produces only maltohexaose from gelatinized starch in the early stage of the reaction. For the production of this amylase potato starch was found to be most effective while soluble sugars including gelatinized starch and maltose had little effect. 相似文献
15.
UDP-(1)fructose was synthesized essentially by the method of Michelson or Roseman et al. The product obtained was much more stable to acid than UDP-fructose isolated from Jerusalem artichoke tubers by Umemura et al.7) and UDP-glucose. Hydrolysis time curves of UDP-(1)fructose and fructose-1-phosphate in 0.01N HGl and 0.1N HCl both at 100°C are presented. It was concluded from these curves that UDP-(1)fructose was first hydrolyzed into UMP and fructose-1-phosphate, and then fructose-1-phosphate was hydrolyzed more slowly into free fructose and inorganic phosphate. 相似文献
16.
Hajime Taniguchi Yasukiyo Umemura Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(2):231-239
The acid-soluble nucleotides were extracted from the tubers of Jerusalem artichoke with percbloric acid, and separated and purified by means of adsorption on and elution from active charcoal, repeated chromatography on columns of Dowex I (Cl-), followed by paper chromatography. The following nucleotides have been characterized and/or identified: 5′-AMP, 3′-AMP, ADP, ATP, 5′-GMP, 2′-GMP, 3′-GMP, 2′,3′-cyclic GMP, GDP, GTP, 5′-UMP, UDP, UTP, NADP, UDP-glucose, UDP-galactose, UDP-fructose, UDP-N-acetylhexosamine and GDP-mannose.** Neither cytosine ribonucleotides nor deoxyribonucleotides have been detected. The significance of these observations is discussed. 相似文献
17.
Michinori Tanaka Shinya Oishi Hiroaki Ohno Nobutaka Fujii 《International journal of peptide research and therapeutics》2007,13(1-2):271-279
A reliable method for solid-phase synthesis of peptide aldehydes by using a new oxazolidine linker is described. Based on
a comparative study using the usual cleavage protocol as is used for the Fmoc-based peptide synthesis, we found that this
new linker is more appropriate for the synthesis of peptide aldehydes compared with the precedent acetal, semicarbazone or
threonine linker. Whereas N-Acylated oxazolidines might be partially deprotected to non-N-acylated intermediates in the TFA cocktail containing several soft nucleophiles which cause significant side reactions, the
new oxazolidine linker could produce the desired peptide aldehydes by simple Et2O washing and subsequent aqueous workup in high chemical yields and purity. We demonstrate the new method is useful especially
for the preparation of highly functionalized long-chain peptide aldehydes which require several scavenger chemicals in the
final deprotection step.
This paper is dedicated to the memory of the late Prof. R. Bruce Merrifield, who passed away May 14, 2006. 相似文献
18.
Ando Shiori Funato Michinori Ohuchi Kazuki Inagaki Satoshi Sato Arisu Seki Junko Kawase Chizuru Saito Toshio Nishio Hisahide Nakamura Shinsuke Shimazawa Masamitsu Kaneko Hideo Hara Hideaki 《Neurochemical research》2019,44(7):1773-1779
Neurochemical Research - Spinal muscular atrophy (SMA) is an inherited disease characterized by progressive motor neuron death and subsequent muscle weakness and is caused by deletion or mutation... 相似文献
19.
Kashiwagi T Hara K Kohara M Iwahashi J Hamada N Honda-Yoshino H Toyoda T 《The Journal of biological chemistry》2002,277(32):28700-28705
20.
Involvement of chalcone reductase in the soybean isoflavone metabolon: identification of GmCHR5, which interacts with 2‐hydroxyisoflavanone synthase 下载免费PDF全文
Ryo Mameda Toshiyuki Waki Yosuke Kawai Seiji Takahashi Toru Nakayama 《The Plant journal : for cell and molecular biology》2018,96(1):56-74
Soybean (Glycine max) 5‐deoxyisoflavonoids (daidzein and its conjugates) are precursors of glyceollin phytoalexins. They are also converted to equol by microbes in the human intestine, resulting in health benefits. 5‐Deoxyisoflavonoids accumulate in the roots (93% mol/mol of the total root isoflavonoids) and seeds of unstressed soybean plants. Chalcone reductase (CHR) is a key enzyme mediating 5‐deoxyisoflavonoid biosynthesis because it catalyzes the production of 6′‐deoxychalcone through its effects on the chalcone synthase (CHS)‐catalyzed reaction. The soybean genome encodes at least 11 CHR‐related homologs, but it is unclear which ones are functionally important for daidzein accumulation in unstressed plants. Among the CHR homologs, the temporal and spatial expression patterns of GmCHR5 were the most correlated with the distribution patterns of 5‐deoxyisoflavonoids. The CHR activity of GmCHR5 was confirmed in vitro and in planta. In the in vitro assays, the ratio of CHR products (6′‐deoxychalcone) to total CHS products (R value) was dependent on GmCHR5 and CHS concentrations, with higher concentrations resulting in higher R values (i.e. approaching 90%). Subcellular localization analyses revealed that GmCHR5 was present in the cytoplasm and nucleus. Protein–protein interaction assays indicated that GmCHR5, but not GmCHR1 and GmCHR6, interacted with 2‐hydroxyisoflavanone synthase (IFS) isozymes. The CHS isozymes also interacted with IFS isozymes but not with GmCHR5. The proposed micro‐compartmentalization of isoflavone biosynthesis through the formation of an IFS‐mediated metabolon is probably involved in positioning GmCHR5 close to CHS, resulting in an R value that is high enough for the accumulation of abundant 5‐deoxyisoflavonoids in soybean roots. 相似文献