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941.
The petH gene, encoding ferredoxin-NADP(+) oxidoreductase (FNR), was isolated from a thermophilic cyanobacterium, Synechococcus elongatus (the same strain as Thermosynechococcus elongatus). The petH gene of S. elongatus was a single copy gene, and the N-terminal region of PetH showed a sequence similarity to the CpcD-phycobilisome linker polypeptide. The amino acid sequence of the catalytic domains of PetH was markedly similar to those from mesophilic cyanobacterial PetH and higher plant FNR. The enzymatically active FNR protein was purified to homogeneity from S. elongatus as three forms corresponding to the 45-kDa form retaining the CpcD-like domain, the 34-kDa form lacking the CpcD-like domain, and the 78-kDa complex with phycocyanin. The FNR in the 78-kDa complex was tolerant to proteolytic cleavage. However, the dissociation of phycocyanin from the 78-kDa complex induced to specific proteolysis between the CpcD-like domain and the FAD-binding domain to give rise to the 34-kDa form of FNR. The enzymatic activity of the 45-kDa form was thermotolerant, but the 45-kDa form readily aggregated under the storage at -30 degrees C. These results suggest that the association with phycocyanin via CpcD-like domain gives remarkable stability to S. elongatus FNR. 相似文献
942.
Study of cognitive function among the Adult Health Study (AHS) population in Hiroshima and Nagasaki 总被引:2,自引:0,他引:2
Yamada M Sasaki H Kasagi F Akahoshi M Mimori Y Kodama K Fujiwara S 《Radiation research》2002,158(2):236-240
Although neuropsychological dysfunction is found among A-bomb survivors exposed in utero as it is among patients who receive central nervous system radiotherapy, neuropsychological examinations have not been conducted on the survivors. Its prevalence may be increased as a result of the increased rate of strokes reported among those exposed to a high radiation dose. In this study, we examined the effects of radiation exposure on cognitive function among adult survivors in the Adult Health Study (AHS). The study subjects were men and women born prior to September 1932 who had undergone biennial examinations during the period 1992-1996 in Hiroshima or 1993-1998 in Nagasaki. We evaluated cognitive performance for 3,113 subjects with the Cognitive Abilities Screening Instrument (CASI), and we examined the relationship between cognitive performance and potentially related factors (sex, age, city where the subjects were exposed, years of education, and radiation dose). In contrast to exposure to radiotherapy, exposure to atomic bomb radiation had no apparent effect on cognitive function. Factors that did affect cognitive function were age, sex, city and years of education. Further investigation, including examination of other neurological functions, is required before a final conclusion regarding radiation-induced neurological dysfunction can be reached. 相似文献
943.
Mohanty A Kathuria H Ferjani A Sakamoto A Mohanty P Murata N Tyagi AK 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,106(1):51-57
Transgenic lines of indica rice were generated by Agrobacterium-mediated transformation with the choline oxidase ( codA) gene from Arthrobacter globiformis. Choline oxidase catalyses conversion of choline to glycine betaine. Glycine betaine is known to provide tolerance against a variety of stresses. Molecular analyses of seven independent transgenic lines as performed by Southern, Northern and Western hybridization revealed integration and expression of the transgene as well as inheritance in the progeny plants. A good correlation was observed between levels of mRNA and protein accumulation, and a significant amount of choline oxidase product, i.e. glycine betaine, accumulated in R0 as well as R1 plants. Mendelian as well as non-Mendelian segregation patterns were obtained in the progeny plants. Challenge studies performed with R1 plants by exposure to salt stress (0.15 M NaCl) for 1 week, followed by a recovery period, revealed that in some cases more than 50% of the transgenic plants could survive salt stress and set seed whereas wild-type plants failed to recover. 相似文献
944.
945.
Gene amplification profiling of esophageal squamous cell carcinomas by DNA array CGH 总被引:6,自引:0,他引:6
Ishizuka T Tanabe C Sakamoto H Aoyagi K Maekawa M Matsukura N Tokunaga A Tajiri T Yoshida T Terada M Sasaki H 《Biochemical and biophysical research communications》2002,296(1):152-155
Gene amplification is one of the basic mechanisms that lead to overexpression of oncogenes. DNA array comparative genomic hybridization (CGH) has great potential for comprehensive analysis of both a relative gene-copy number and altered chromosomal regions in cancers, which enables us to identify new amplified genes and unstable chromosomal loci. We examined the amplification status in 32 esophageal squamous cell carcinomas (ESCCs) and 13 ESCC cell lines on 51 frequently amplified loci in a variety of cancers by both DNA array CGH and Southern blot analyses. The 1p34 locus containing MYCL1, 2p24 (MYCN), 7p12 (EGFR), and 12q14 (MDM2) were amplified in one of the 32 cases (3%), and the 17q12 locus (ERBB2) and 8p11 (FGFR1) in two of the 32 cases (6%), while only the 11q13 locus (Cyclin D1, FGF4, and EMS1) was frequently amplified (28%, 9/32), demonstrating this locus to be a major target in ESCCs. One locus, 8q24 (c-MYC) was found to be amplified only in the cell lines. Eight out of 51 loci (15.7%) were found to be amplified in at least one of the 32 primary ESCCs or the 13 ESCC cell lines, suggesting that chromosomal loci frequently amplified in a type of human cancer may also be amplified in other types of cancers. This paper is the first report of an application of DNA array CGH to ESCCs. 相似文献
946.
Yamaoka J Kabashima K Kawanishi M Toda K Miyachi Y 《Biochemical and biophysical research communications》2002,291(4):780-786
Endothelial cell injury is a critical event in tissue damage accompanying inflammation, in which both inflammatory cytokines and reactive oxygen species may play pivotal roles, although the exact mechanism has not yet been clarified. We found that combined stimulation with interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) induced both cytotoxicity to murine vascular endothelial cell line F-2 and an increase in nitric oxide (NO). Therefore, in the present study, the implication of NO in cytotoxicity was examined. A potent iNOS-specific inhibitor ONO-1714 completely blocked both cytokine-induced cytotoxicity and NO production. NO scavengers such as carboxy-PTIO and hemoglobin blocked cytotoxicity. Moreover, exogenous NO from NOC 18 also caused cytotoxicity. These results together demonstrated that cytotoxicity of IFN-gamma and TNF-alpha for endothelial cell F-2 was mediated by NO, suggesting a pathogenic role of cytokine-induced NO production in endothelial damage under inflammatory conditions. 相似文献
947.
Regulation of the desaturation of fatty acids and its role in tolerance to cold and salt stress 总被引:2,自引:0,他引:2
The expression of cold-inducible genes is regulated by a two-component system in Synechocystis and Bacillus subtilis. The cold sensors are membrane-bound histidine kinases and it seems likely that they sense and transduce changes in the fluidity of membranes. Desaturation of fatty acids in membrane lipids has been implicated in tolerance to cold and salt stress. 相似文献
948.
M. Sakamoto Jiro Ono Shintaro Okada Mitsuo Masuno Yusuke Nakamura Hiroki Kurahashi 《Human genetics》1998,103(5):586-589
LIS1 is a genetic entity that is responsible for lissencephaly. Previously we have reported isolated lissencephaly sequence(ILS)
in a Japanese patient carrying a balanced chromosomal translocation that disrupted the LIS1 gene. We examined mutations of LIS1 in 12 additional Japanese patients, 8 of them with ILS and 4 with Miller-Dieker syndrome (MDS). Fluorescence in situ hybridization
(FISH) analysis disclosed deletions of part of the LIS1 gene or of the chromosomal region surrounding it in three of the ILS cases and in three of the MDS cases. In one of the remaining
five ILS cases, SSCP analysis and subsequent sequence analysis identified a 1-bp deletion in exon IV, which can be expected
to result in premature termination of the gene product. Our results indicate that in Japan, as elsewhere, abnormality of the
LIS1 gene is a common cause of MDS/ILS.
Received: 20 April 1998 / Accepted: 28 July 1998 相似文献
949.
Matsumoto A Matsumoto N Harui Y Sakamoto M Tomioka K 《Journal of insect physiology》1998,44(7-8):587-596
In the wild type (Canton-S) and period mutant flies of Drosophila melanogaster, we examined the effects of light and temperature on the circadian locomotor rhythm. Under light dark cycles, the wild type and per(S) flies were diurnal at 25 degrees C. However, at 30 degrees C, the daytime activity commonly decreased to form a rather nocturnal pattern, and ultradian rhythms of a 2 approximately 4h period were observed more frequently than at 25 degrees C. The change in activity pattern was more clearly observed in per(0) flies, suggesting that these temperature dependent changes in activity pattern are mainly attributable to the system other than the circadian clock. In a 12h 30 degrees C:12h 25 degrees C temperature cycle (HTLT12:12), per(0) flies were active during the thermophase in constant darkness (DD) but during the cryophase in constant light (LL). The results of experiments with per(0);eya flies suggest that the compound eye is the main source of the photic information for this reversal. Wild type and per(0) flies were synchronized to HTLT12:12 both under LL and DD, while per(S) and per(L) flies were synchronized only in LL. This suggests that the circadian clock is entrainable to the temperature cycle, but the entrainability is reduced in the per(S) and per(L) flies to this particular thermoperiod length, and that temperature cycle forces the clock to move in LL, where the rhythm is believed to be stopped at constant temperature. 相似文献
950.