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排序方式: 共有376条查询结果,搜索用时 15 毫秒
371.
Minoru Takizawa Tomoko Nomura Etsuji Wakisaka Naonobu Yoshizuka Junken Aoki Hiroyuki Arai Keizo Inoue Michihiro Hattori Noboru Matsuo 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》1999,1438(2):301-304
Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycolipids in mammals. We have isolated and sequenced the cDNA clone encoding human lactosylceramide synthase. The deduced amino acid sequence of the human lactosylceramide synthase showed 94.2% identity with rat lactosylceramide synthase. Northern blotting analysis revealed that lactosylceramide synthase mRNA was expressed in various tissues, with the highest level in brain and adrenal gland. 相似文献
372.
Koichiro Suemori Masayuki Saijo Atsushi Yamanaka Daisuke Himeji Masafumi Kawamura Takashi Haku Michihiro Hidaka Chinatsu Kamikokuryo Yasuyuki Kakihana Taichi Azuma Katsuto Takenaka Toru Takahashi Akitsugu Furumoto Toshiyuki Ishimaru Masayuki Ishida Masahiko Kaneko Norimitsu Kadowaki Kenichi Ikeda Shigetoshi Sakabe Tomohiro Taniguchi Hiroki Ohge Takeshi Kurosu Tomoki Yoshikawa Masayuki Shimojima Masaki Yasukawa 《PLoS neglected tropical diseases》2021,15(2)
Severe fever with thrombocytopenia syndrome (SFTS) is a bunyavirus infection with high mortality. Favipiravir has shown effectiveness in preventing and treating SFTS virus (SFTSV) infection in animal models. A multicenter non-randomized, uncontrolled single arm trial was conducted to collect data on the safety and the effectiveness of favipiravir in treatment of SFTS patients. All participants received favipiravir orally (first-day loading dose of 1800 mg twice a day followed by 800 mg twice a day for 7–14 days in total). SFTSV RT-PCR and biochemistry tests were performed at designated time points. Outcomes were 28-day mortality, clinical improvement, viral load evolution, and adverse events (AEs). Twenty-six patients were enrolled, of whom 23 were analyzed. Four of these 23 patients died of multi-organ failure within one week (28-day mortality rate: 17.3%). Oral favipiravir was well tolerated in the surviving patients. AEs (abnormal hepatic function and insomnia) occurred in about 20% of the patients. Clinical symptoms improved in all patients who survived from a median of day 2 to day10. SFTSV RNA levels in the patients who died were significantly higher than those in the survivors (p = 0.0029). No viral genomes were detectable in the surviving patients a median of 8 days after favipiravir administration. The 28-day mortality rate in this study was lower than those of the previous studies in Japan. The high frequency of hepatic dysfunction as an AE was observed. However, it was unclear whether this was merely a side effect of favipiravir, because liver disorders are commonly seen in SFTS patients. The results of this trial support the effectiveness of favipiravir for patients with SFTS. 相似文献
373.
Shinji Sugiura Shinya Yamahira Masato Tamura Kazumi Shin Mayu Shibuta Taku Satoh Yui Matsuzawa Gen Fujii Fumiki Yanagawa Michihiro Mutoh Masumi Yanagisawa Ryuji Kato Hirofumi Matsui 《Biotechnology and bioengineering》2023,120(6):1492-1505
We report an automated cell-isolation system based on fluorescence image analysis of cell aggregates cultured in a photodegradable hydrogel. The system incorporates cell culture in a humidified atmosphere with controlled CO2 concentration and temperature, image acquisition and analysis, micropatterned light exposure, and cell collection by pipetting. Cell aggregates were cultured on hydrogels, and target cells were selected by phase contrast and fluorescence image analysis. After degradation of the hydrogel by exposure to micropatterned UV light, cell aggregates were transferred to a collection vessel by robotic pipetting. We assessed the system for hydrogel degradation, recovery of target cells, and contamination by off-target cells. We demonstrated two practical applications of our method: (i) in cell aggregates from MCF-7-RFP strains in which 18.8% of cells produced red fluorescent protein (RFP), we successfully obtained 14 proliferative fluorescence-positive cell aggregates from 31-wells, and all of the isolated strains produced a higher proportion of RFP production than the original populations; (ii) after fluorescent immunostaining of human epidermal growth factor receptor 2 (HER2) in cancer cells, we successfully isolated HER2-positive cells from a mixed population of HER2-positive and -negative cells, and gene sequence analysis confirmed that the isolated cells mainly contained the target cells. 相似文献
374.
Phosphatidylcholine–cholesterol acyltransferase in the ultracentrifugal residual protein fraction of rat plasma 下载免费PDF全文
Michihiro Sugano 《The Biochemical journal》1971,122(4):469-475
1. The phosphatidylcholine-cholesterol acyltransferase of rat plasma was dissociated from the plasma lipoproteins by ultracentrifugation and shown to be present in the plasma residual-protein fraction of density >1.210. 2. The general properties of the acyltransferase were substantially unchanged in the residual fraction as judged from the effects of differences in the substrates and of overnight starvation on the formation of different cholesterol esters. 3. The enzyme from rats starved overnight, by comparison with the enzyme from fed rats, preferentially formed cholesteryl arachidonate at the expense of cholesteryl linoleate. 4. The results suggest that ultracentrifugal separation of the plasma residual fraction may be used as an initial step for the purification of the acyltransferase. 相似文献
375.
Rana Ashkar Michihiro Nagao Paul?D. Butler Andrea?C. Woodka Mani?K. Sen Tadanori Koga 《Biophysical journal》2015,109(1):106-112
Membrane thickness fluctuations have been associated with a variety of critical membrane phenomena, such as cellular exchange, pore formation, and protein binding, which are intimately related to cell functionality and effective pharmaceuticals. Therefore, understanding how these fluctuations are controlled can remarkably impact medical applications involving selective macromolecule binding and efficient cellular drug intake. Interestingly, previous reports on single-component bilayers show almost identical thickness fluctuation patterns for all investigated lipid tail-lengths, with similar temperature-independent membrane thickness fluctuation amplitude in the fluid phase and a rapid suppression of fluctuations upon transition to the gel phase. Presumably, in vivo functions require a tunability of these parameters, suggesting that more complex model systems are necessary. In this study, we explore lipid tail-length mismatch as a regulator for membrane fluctuations. Unilamellar vesicles of an equimolar mixture of dimyristoylphosphatidylcholine and distearoylphosphatidylcholine molecules, with different tail-lengths and melting transition temperatures, are used as a model system for this next level of complexity. Indeed, this binary system exhibits a significant response of membrane dynamics to thermal variations. The system also suggests a decoupling of the amplitude and the relaxation time of the membrane thickness fluctuations, implying a potential for independent control of these two key parameters. 相似文献
376.
Tsuyoshi Hosoya Kiyoshi Hamano Michihiro Sugano Yoko Ogura Emiko Hatano Takakazu Hamada 《Mycoscience》1999,40(6):525-529
Dicephalosterol, a new testosterone 5α-reductase inhibitor, was found from isolates ofDicephalospora rufocornea, a sclerotiniaceous discomycete widely distributed, but not previously cultured. Under SEM observation, the polar appendage
of the ascospores inD. rufocornea was found to be more solid than was hitherto reported. Dicephalosterol was produced by submerged fermentation for 7 d. This
new analogue of testosterone showed an IC50 of 5.7 μg/ml for rat prostatic 5α-reductase, but no antimicrobial activity against bacteria or fungi. 相似文献