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Quintela-Sabarís Celestino Auber Etienne Sumail Sukaibin Masfaraud Jean-François Faucon Michel-Pierre Watteau Françoise Saad Ramez F. van der Ent Antony Repin Rimi Sugau John Nilus Reuben Echevarria Guillaume Leguedois Sophie 《Plant and Soil》2019,435(1-2):201-224
Plant and Soil - Non- or minimally invasive methods are urgently needed to characterize and monitor crop root systems to foster progress in phenotyping and general system understanding. Electrical... 相似文献
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Jean-Louis Dasseux Jean-François Faucon Michael Lafleur Michel Pezolet Jean Dufourcq 《生物化学与生物物理学报:生物膜》1984,775(1):37-50
Perturbations induced by melittin on the thermotropism of dimyristoyl-, dipalmitoyl-, distearoylphosphatidylcholine and natural sphingomyelin are investigated and rationalized from data obtained by fluorescence polarization, differential scanning calorimetry and Raman spectroscopy. Depending on the technique and / or experimental conditions used, the observed effects differ at the same lipid to protein molar ratio, due to partial binding of melittin. The binding is more efficient for tetrameric than for monomeric melittin, but in both cases its affinity is weaker for phosphatidylcholine dispersions in the gel phase than for sonicated vesicles. For temperatures efficient binding occurs whatever the initial state of the lipids is. One can summarize the effects induced by melittin on the transition temperature as follows: (i) No upward shift is observed on synthetic phosphatidylcholines when lipid degradation is avoided. This is achieved by using highly purified melittin, phospholipase inhibitors, and / or non-hydrolysable lipids. (ii) Melittin monomer does not change . (iii) When melittin tetramer is stabilized, it decreases by 10–15 deg. C. The transition broadens, and is finally abolished for . Very similar results are found for natural sphingomyelin. Fluorescence polarization indicates similar changes in order and dynamics of the acyl chains for all lipid studied. For , fluorescence and Raman show that melittin decreases the amount of CH2 groups in ‘trans’ conformation and the intermolecular order of the chains. According to fluorescence data, there is an increase of the rigid-body orientational order at , while from Raman the positional intermolecular order decreases without significant change in the CH2 groups ‘trans’/‘gauche’ ratio. 相似文献
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J. F. Coulon N. Faucon Biguet A. Cavoy † J. Delacour † J. Mallet J. C. David 《Journal of neurochemistry》1990,55(4):1412-1417
Tyrosine hydroxylase, aromatic L-amino-acid decarboxylase, and dopamine beta-hydroxylase activities were studied in the developing fetal rat brain. A delay of 2-3 days between the detection of the tyrosine hydroxylase and the aromatic L-amino-acid decarboxylase and dopamine beta-hydroxylase activities was observed. For this reason, the expression of tyrosine hydroxylase mRNA was studied. Tyrosine hydroxylase mRNA was visualized in the whole brain from 13 days of gestation, but the largest increase of the expression was observed in the hypothalamus. These results are discussed in terms of the relative gene expressions of the three enzymes involved in the biosynthesis of catecholamines and phenolamines in nervous tissues. 相似文献
55.
Jean-Claude Talbot Jacqueline Lalanne Jean-François Faucon Jean Dufourcq 《生物化学与生物物理学报:生物膜》1982,689(1):106-112
In solutions of increasing ionic strength, the molecular weight of melittin varies from 2840 (monomeric melittin) to 11 200. This polymerization, concomitant with an important change in conformation (Talbot, J.C., Dufourcq, J., De Bony, J., Faucon, J.F. and Lussan, C. (1979) FEBS Lett. 102, 191–193), is accompanied by a significant alteration in the partial specific volume of the molecule. The binding of melittin to phospholipids (phosphatidylserine, lysolecithin, dihexanoyl-, dioctanoyl- and lysolauroylphosphatidylcholine) depends on the state of association of the toxin and on the critical micelle concentration of lipids. No interaction is observed between monomeric melittin and free lipids, whereas tetrameric melittin can bind free lipids to form mixed micelles. At phospholipid concentrations above the critical micelle concentration, melittin in any state of self-association can bind lipids. The mixed micelles formed at saturation appear to be independent of the initial state of association of melittin. 相似文献
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Implication of plant-soil relationships for conservation and restoration of copper-cobalt ecosystems