Development and characterization of a human cell line from an ovarian mixed mullerian tumor (carcinosarcoma)

Summary A cell line derived from a human ovarian carcinosarcoma was established in tissue culture and in nude mice. Two sublines, LDF and HDF, separated by discontinuous density centrifugation were also established from the parent line JoN. The cloning efficiency of the JoN line was 21%. Morphologic features of adenocarcinoma cells characteristic of the parent JoN cells were retained in the sublines and clones; all lines showed the same karyotype and DNA content (pseudodiploid and pseudotetraploid). Keratin, as demonstrated immunohistochemically, was strongly expressed in the parent line JoN and the xenograft tumor, but not at all in the LDF sublines and only moderately in the HDF sublines. Vimentin, however, was expressed in neither the parent line JoN nor the xenograft tumor, but was present in both sublines. Transglutaminase and plasminogen activator activity was high in the parent line JoN. Neither, sublines nor clones showed the same high enzyme activity as the parent line. It is concluded that this human tumor line JoN is comprised of epithelial cells, capable of multidirectional differentiation.     

Oral behaviour following forebrain ablations in Gallus Domesticus

The oral behaviour of adult Brown Leghorn hens was recorded in response to oral stimulation with water, 2 M sodium chloride, 2 M acetic acid and 0.1 M quinine hydrochloride before and after surgical ablation of either the anterior telencephalon or the entire telencephalon and/or the diencephalon. It was found that beak wiping behaviour could be abolished by the removal of the anterior telencephalon. Head shaking behaviour was abolished only by the complete removal of the forebrain (telencephalon and diencephalon) whereas beak and tongue movements persisted after forebrain removal. Although these three behaviour patterns occur together in response to the stimulus, they appear to be controlled in different areas of the brain. The results are discussed in relation to the current work on the control of oral behaviour in mammals.