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951.
952.
John H. Pazur Michael E. Tay Beverly A. Pazur Frank J. Miskiel 《Journal of Protein Chemistry》1987,6(5):387-399
Sets of isomeric anti-lactose antibodies with specificity for the lactose units of a cell wall polysaccharide fromStreptococcus faecalis strain N were induced in rabbits immunized with a vaccine of nonviable cells of the organism. Such sets of anti-lactose antibodies
were isolated from the serum of immunized animals by affinity chromatography on lactosyl-Sepharose. Gel electrofocusing experiments
showed that the preparations consisted of multiprotein components. One preparation of antibodies of 13 isomers was separated
into homogeneous components by liquid isoelectrofocusing. The individual isomeric antibodies exhibit specificity for the lactose
units of the antigenic polysaccharide, possess isoelectric points in the range of 5.9–8.0, and belong to the IgG class of
immunoglobulins, and each member yields one light chain and one heavy chain on dissociation in sodium dodecyl sulfate (SDS)
and mercaptoethanol. These results have been interpreted as evidence for the assembly of the chains of isomeric antibodies
by a single-chain pairing mechanism. 相似文献
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Michael J. Orlove 《Journal of theoretical biology》1982,96(2):107-127
A model is demonstrated with three genetic. loci. One is an altruism/selfishness allele pair, the other two affect individual fitness. Two main studies were conducted. One tried to repeat an experiment by Stroebeck, Maynard Smith and Charlesworth which showed genes for high crossover rates can hitchhike up more often than down. But here the altruism/selfishness locus replaced the recombination-rate locus. The other started out with “good, bad” and “bad, good” chromosomes looking to see if altruism would hitchhike, up on “good, good” or down on “bad, bad”, or not hitchhike at all, once crossing over has been going on. In all experiments altruism tended to hitchhike down on the most deleterious genotypes available at the other loci. This means the current study failed to shed light on the question of the cost of oogenesis. 相似文献
956.
Ian D. Stephen Miriam J. Law Smith Michael R. Stirrat David I. Perrett 《International journal of primatology》2009,30(6):845-857
Numerous researchers have examined the effects of skin condition, including texture and color, on the perception of health,
age, and attractiveness in human faces. They have focused on facial color distribution, homogeneity of pigmentation, or skin
quality. We here investigate the role of overall skin color in determining perceptions of health from faces by allowing participants
to manipulate the skin portions of color-calibrated Caucasian face photographs along CIELab color axes. To enhance healthy
appearance, participants increased skin redness (a*), providing additional support for previous findings that skin blood color enhances the healthy appearance of faces. Participants
also increased skin yellowness (b*) and lightness (L*), suggesting a role for high carotenoid and low melanin coloration in the healthy appearance of faces. The color preferences
described here resemble the red and yellow color cues to health displayed by many species of nonhuman animals. 相似文献
957.
Gretchen A Casazza Kevin A Jacobs Sang-Hoon Suh Benjamin F Miller Michael A Horning George A Brooks 《Journal of applied physiology》2004,97(1):302-309
We examined the effects of menstrual cycle phase and oral contraceptive (OC) use on triglyceride mobilization during 90 min of rest and 60 min of leg ergometry exercise at 45 and 65% peak O(2) uptake (Vo(2 peak)) in eight moderately physically active, eumenorrheic women (24.8 +/- 1.2 yr). Subjects were tested during the follicular phase (FP) and the luteal phase (LP) before OC use and during the inactive phase (IP) and high-dose phase (HP) after 4 complete mo of OC use. Glycerol rate of appearance (R(a)), a measure of triglyceride mobilization, was determined in a 3-h postabsorptive state using a primed constant infusion of [1,1,2,3,3-(2)H]glycerol. Before OC use (BOC), there were no significant differences between FP and LP in any of the variables studied. Dietary composition, exercise patterns, plasma glycerol concentrations, growth hormone concentrations, and exercise respiratory exchange ratio did not change with OC use. However, 4 mo of OC use significantly (P < 0.05) increased glycerol R(a) in HP during exercise at 45% Vo(2 peak) (6.2 +/- 0.2, 6.5 +/- 0.4, and 7.7 +/- 1.1 micromol.kg(-1).min(-1) for BOC, IP, and HP, respectively) and in IP and HP at 65% Vo(2 peak) (6.6 +/- 0.1, 8.2 +/- 0.6, and 8.1 +/- 0.7 micromol.kg(-1).min(-1) for BOC, IP, and HP, respectively). Plasma cortisol concentrations were significantly higher with OC use at rest and during exercise at 45 and 65% Vo(2 peak). In summary, although fluctuations of endogenous ovarian steroids have little effect on triglyceride mobilization, the synthetic ovarian steroids found in OCs increase triglyceride mobilization and plasma cortisol concentrations in exercising women. We conclude that the hierarchy of effects of ovarian steroids and their analogs on triglyceride mobilization in exercising women is as follows: energy flux > OC use > recent carbohydrate nutrition, menstrual cycle effects. 相似文献
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Nb1-particles fromNitrobacter agilis were found to be highly stable and could only be disrupted by chemicals or prolonged sonication.Spectra of the Nb1-particles indicated that protein is their major component. They contain no lipid.Highly purified Nb1-particles that were electronmicroscopically free from contaminating membranes, contained 7 different proteins, as shown by sodium dodecyl sulfate-polyacrylamide gelelectrophoresis.Abbreviations SDS
sodium dodecyl sulfate
- PAGE
polyacrylamide-gelelectrophoresis
- M. W.
molecular weight
- O.D.
opitical density
- HAA
hepatitis associated antigen 相似文献