Comparison of chemical analyses of boat and helicopter-collected water samples

Helicopters can be used to collect water samples from many lakes over a wide geographic area within a relatively short time period. Here we report the results from an experiment in which sequential water samples from a lake were collected first from a nonmotorized boat and then immediately afterward from a helicopter. No significant differences were found between the means of the measurement of 20 chemical parameters for the two methods of collection. When compared to obtaining samples from a boat, collection of samples from a helicopter platform had no effect on the content of the water samples.     

A new facultatively nitrite oxidizing bacterium,Nitrobacter vulgaris sp. nov.

A total of 17 facultatively lithoautotrophic strains of Nitrobacter were investigated. They all were found to be related on the species level by DNA hybridizations. The G+C content of DNA ranged between 58.9 and 59.9 mol %. The isolates originated from divers environments. The cells were 0.5–0.8×1.2–2.0 m in size and motile by one polar to subpolar flagellum. Cell-division normally occurred by budding. Polar caps of intracytoplasmic membranes as well as carboxysomes were present. The cells tended to excrete extracellular polymers forming aggregates or biofilms. Heterotrophic growth was slower than mixotrophic but often faster than litoautotrophic growth. In the presence of nitrite and organic substances the organisms often showed diphasic growth. First nitrite and then the organic material was oxidized. In the absence of oxygen growth was possible by dissimilatory nitrate reduction. Nitrite, nitric and nitrous oxide as well as ammonia were formed. Depending on growth conditions the generation times varied from 12 to 140 h. The new Nitrobacter spec. may be one of the most abundant nitrite-oxidizing bacteria in soils, fresh waters and natural as well as artificial stones. For this organism the name Nitrobacter vulgaris is proposed.The type strain is filed with the culture collection of the Institut für Allgemeine Botanik, Universität Hamburg, FRG.     

Nasal trigeminal responses to toluene presented by an automated delivery system

An apparatus was developed which permits the automated deliveryof volatile chemical stimuli for use in neurophysiology experiments.A computer-controlled olfactometer, incorporating electronicmass flow controllers (EMFCs) and Teflon-lined solenoid valves,generated and delivered clean or odorized air. Neural and respiratorysignals from the animal were amplified and stored, along withtrial information (e.g. odorant concentration) on a chart recorderand video cassette recorder, both of which were controlled bythe computer. This apparatus was used to measure responses totoluene from the rat ethmoid nerve, a part of the ophthalmicdivision of the trigeminal nerve. Multi-unit responses to thiscompound were first observed at 2000 p.p.m. The magnitude ofthe response increased linearly with logarithmic increases inconcentration up to vapour saturation. Changes in respirationin response to toluene also were observed, although neural responsesoften were seen in the absence of respiratory changes.     

Preparation and characterisation of monoclonal and polyclonal antibodies to maize membrane auxin-binding protein

Binding proteins, thought to be auxin receptors, can be solubilised from maize (Zea mays L.) membranes after acetone treatment. From these crude extracts, receptor preparations of over 50% purity can be obtained by a reliable, straight-forward procedure involving three chromatographic steps — anion exchange, gel filtration and high-resolution anion exchange. Such preparations have been used to immunise rats for subsequent production of monoclonal antibodies. By the further step of native polyacrylamide gel electrophoresis the semi-purified preparations yield homogeneous, dimeric (22-kilodalton, kDa) auxin-binding protein, which has been used to produce a polyclonal rabbit antiserum. The preliminary characterisation of this antiserum and of the five monoclonal antibodies is presented. Two of the monoclonal antibodies specifically recognise the major 22-kDa-binding protein polypeptide whilst the other three recognise, in addition, a minor 21-kDa species. All the monoclonal antibodies recognise the polypeptide rather than the glycan side chain and the polyclonal antiserum also recognises deglycosylated binding protein. The antibodies have been used to quantify the abundance of auxinbinding protein in a number of tissues of etiolated maize seedlings. Root membranes contain 20-fold less binding protein than coleoptile membranes.Abbreviations ABP auxin-binding protein - DEAE diethylaminoethyl - Ig immunoglobulin - kDa kilodalton - NAA naphthalene-1-acetic acid - M_r relative molecular mass - PAGE polyacrylamide gel electrophoresis - SDS sodium dodecyl sulfate     

A distributed knowledge-based approach to flexible automation: The contract net framework

This article applied distributed artificial intelligence to the real-time planning and control of flexible manufacturing systems (FMS) consisting of asynchronous manufacturing cells. A knowledge-based approach is used to determine the course of action, resource sharing, and processor assignments. Within each cell there is an embedded automatic planning system that executes dynamic scheduling and supervises manufacturing operations. Because of the decentralized control, real-time task assignments are carried out by a negotiation process among cell hosts. The negotiation process is modeled by augmented Petri nets —the combination of production rules and Petri nets—and is excuted by a distributed, rule-based algorithm.     

Morphological dispersion of introduced Hawaiian finches: evidence for competition and a Narcissus effect

Summary Morphological analyses were conducted on finch species introduced to Oahu, Hawaii. As many as 25 species of finches representing four families (Emberizidae, Fringillidae, Ploecidae, Estrildidae) have been introduced to Oahu. Of these, 15 species currently have established wild populations. When compared with pools of 25 and 23 species introduced to Oahu, the 15 surviving species are morphologically overdispersed. This result is consistent with the hypothesis that competition has influenced the outcome of these introductions. We also tested the hypothesis that surviving introduced finches in one habitat (sugarcane fields) were morphologically overdispersed. When the seven surviving finch species found in sugarcane were compared with the pools of 25 and 23 species, they too were morphologically overdispersed. However, when the seven sugarcane species were compared with a species pool consisting of only the surviving 15 species found across Oahu, they were not morphologically overdispersed. This result suggests that morphological analyses of community structure based only on comparisons of assemblages of surviving species may be biased by a Narcissus effect.     

Internal seiches in Lake Constance: influence on plankton abundance at a fixed sampling site

During thermal stratification, pronounced internal seiches occurin Lake Constance with periods of 4–6 days The amplitudesreach 12 m at the sampling site in berlinger See (northwesternpart of Lake Constance). Seiches change the thickness of thewarm and generally biologically rich epilimnion and, thus, alsothe areal abundance of planktonic organisms at a fixed samplingsite. The potential impact of seiche-dnven changes of epilimneticthickness on observed plankton abundance was calculated usingcontinuously recorded temperature profiles and weekly verticalprofiles of plankton biomass. Observed plankton biomass wasrecalculated by taking into account the effect of changing layerthickness in order to give a more realistic picture of biomasschanges caused by waxing and waning of populations Our calculationsimply that changes of strata thickness do not mask the generalseasonal trend in population dynamics (e g. spring bloom andclear water phase), estimates of average yearly standing stockare not significantly affected if sampling is done at leastweekly. However, changes of strata thickness can alter arealplankton abundance at the sampling site by a factor of 2 within2 days During pronounced seiches, areal abundance of phytoplankton,which generally exhibit a stronger vertical gradient than-mostother plankton, may be changed by a factor of 3–4 withinhours Hence, at our sampling site, the impact of changes instrata thickness cannot be ignored for detailed analysis ofpopulation dynamics of autotrophs involving comparisons betweensingle observations     

Stimulation of insulin release from pancreatic islets by quinones

Coenzyme Q (CoQ₀) and other quinones were shown to be potent insulin secretagogues in the isolated pancreatic islet. The order of potency was CoQ₀benzoquinonehydroquinonemenadione. CoQ₆ and CoQ₁₀ (ubiquinone), duroquinone and durohydroquinone did not stimulate insulin release. CoQ₀'s insulinotropism was enhanced in calcium-free medium and CoQ₀ appeared to stimulate only the second phase of insulin release. CoQ₀ inhibited inositol mono-, bis- and trisphosphate formation. Inhibitors of mitochondrial respiration (rotenone, antimycin A, FCCP and cyanide) and the calcium channel blocker verapamil, did not inhibit CoQ₀-induced insulin release. Dicumarol, an inhibitor of quinone reductase, did not inhibit CoQ₀-induced insulin release, but it did inhibit glucose-induced insulin release suggesting that the enzyme and quinones play a role in glucose-induced insulin release. Quinones may stimulate insulin release by mimicking physiologically-occuring quinones, such as CoQ₁₀, by acting on the plasma membrane or in the cytosol. Exogenous quinones may bypass the quinone reductase reaction, as well as many reactions important for exocytosis.     

The Isolation and Evaluation of Two Naturally Occurring Mild Strains of Vanilla Necrosis Potyvirus for Control by Cross-Protection

In an attempt to find mild virus strains that would cross-protect sgainst vanilla necrosis potyvirus (VNPV), Vanilla fragrans plants in Tonga were surveyed for the presence of mild or symptomless potyvirus infections. Potyviruses were detected by indirect ELISA using a commercially available portyvirus group monoclonal anibody. From 28 plants with mild or symptomless infections two portyvirus isolates, designated V1 and V3, included systemic infections in Nicotiana benthamiana following mechanical inoculation. V1, which causes a mild mottle in N. benthamiana, is serologically related to VNPV, while V3 which causes mild vein banding is serologically unrelated to VNPV. Prior inoculation with V1 protected N. benthamiana against the severe mosaic symptoms of VNPV when challenge inoculated after 14 and 21 days, but not after 7 days. When V3 was used as the protecting strain, cross-protection was observed in some, but not all plants, when chalenged with VNPV after 14 and 21 days.