A soluble chloroplast protein catalyzes ribulosebisphosphate carboxylase/oxygenase activation in vivo

Ribulosebisphosphate carboxylase/oxygenase (EC 4.1.1.39) (rubisco) must be fully activated in order to catalyze the maximum rates of photosynthesis observed in plants. Activation of the isolated enzyme occurs spontaneously, but conditions required to observe full activation are inconsistent with those known to occur in illuminated chloroplasts. Genetic studies with a nutant of Arabidopsis thaliana incapable of activating rubisco linked two chloroplast polypeptides to the activation process in vivo. Using a reconstituted light activation system, it was possible to demonstrate the participation of a chloroplast protein in rubisco activation. These results indicate that a specific chloroplast enzyme, rubisco activase, catalyzes the activation of rubisco in vivo.     

Oral behaviour following forebrain ablations in Gallus Domesticus

The oral behaviour of adult Brown Leghorn hens was recorded in response to oral stimulation with water, 2 M sodium chloride, 2 M acetic acid and 0.1 M quinine hydrochloride before and after surgical ablation of either the anterior telencephalon or the entire telencephalon and/or the diencephalon. It was found that beak wiping behaviour could be abolished by the removal of the anterior telencephalon. Head shaking behaviour was abolished only by the complete removal of the forebrain (telencephalon and diencephalon) whereas beak and tongue movements persisted after forebrain removal. Although these three behaviour patterns occur together in response to the stimulus, they appear to be controlled in different areas of the brain. The results are discussed in relation to the current work on the control of oral behaviour in mammals.     

Chemiluminescence associated with phagocytosis of foreign particles in rabbit alveolar macrophages

Rabbit alveolar macrophages exhibit a chemiluminescent response which is associated with phagocytosis of zymosan and polystyrene-butadiene particles. The chemiluminescence reaches a peak in 15 to 25 minutes and then gradually diminishes over the next 1 to 3 hours. During the time of maximal light emission there appears to be no actual uptake of particles, but the response is dependent upon the particle concentration. The metabolic inhibitor, DNP (2,4-dinitrophenol), causes a rapid inhibition of the chemiluminescent response. The addition of ATP to the medium prior to exposure of the cells to particles causes the chemiluminescent response to be greatly diminished, i.e., 0.3mM ATP virtually abolishes the response. These experiments suggest that some metabolic response of the cell to phagocytosis is responsible for the chemiluminescence.