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41.
The response of human newborn lymphocytes in autologous mixed lymphocyte culture was examined for specificity (by restimulation), responder cell phenotype, and responder cell frequency. When the newborn T cells were separated from non-T cells by rosetting with sheep erythrocytes (E) in fetal calf serum (FCS), approximately 1:20,000 T cells proliferated. These responders had specificity for E + FCS, were T4+, and were self-restricted. Significant numbers of responder T cells were not found when newborn T and non-T cells were separated by nylon wool. Responses in parallel autologous cultures of adult T cells showed that 1) adults had a higher frequency than newborns of E + FCS specific responders, 2) evidence for self specificity was lacking in restimulated cultures, and 3) occasional responses to antigen on the surface of monocytes could not be excluded. 相似文献
42.
Hostile intercommunity relations, including attacking and killing extra-community infants of both sexes have occurred at most
wild chimpanzee sites. We describe three recent cases of intercommunity attacks on infants committed by members of the Ngogo
chimpanzee community in Kibale National Park, Uganda. Two of the attacks resulted in confirmed infanticides while a third
attack probably resulted in the infant's death. In common with previous accounts of chimpanzee infanticides, the attacks described
here occurred during boundary patrols outside the Ngogo community's usual range, adult and adolescent males were the main
participants, one infant was cannibalized after being killed, and the victims’ mothers did not accompany the attacking party
back to the Ngogo range. However, the patrol parties during each infanticide were larger than before and included females
from the Ngogo community. Our observations indirectly support both the range expansion and imbalance of power hypotheses,
which address why and under which conditions chimpanzee intercommunity encounters lead to aggression. These cases of intercommunity
infanticide add to the growing database of the phenomenon in wild chimpanzees. 相似文献
43.
Summary The results of a recent quantitative analysis of the Teorell membrane oscillator are utilized to explore its role as an excitability analogue. Special attention is paid to its role as a mechano-electric transducer. A membrane of exceptionally well-defined pore structure has been used in this study. The analogue properties arise from nonlinear coupling between water and salt fluxes. When the membrane is simultaneously subjected to controlled gradients of hydrostatic pressure, electrical potential and concentration, bi-stable stationary states can be produced. These arise from the opposing effects of pressure and electro-osmosis on the volume flow. Transitions between these states show hysteresis. The factors governing such transitions are analogous to certain types of stimuli encountered in the natural excitation process. The membrane system also shows oscillatory behavior when the hydrostatic pressure gradient is allowed to vary under constant current conditions. This property is related to the bi-stable stationary state phenomena and is compared to the regenerative behavior found in biologically excitable tissues. Particular emphasis is placed upon analogies between the membrane oscillator and certain natural tissues. The importance of the nonlinear nature of the force-flux coupling in the analogue is stressed, and its possible relevance to biological excitability indicated. Some consideration is also given to the role of electro-osmotic flux coupling in biological tissues. 相似文献
44.
D R Garrod 《Experimental cell research》1972,72(2):588-591
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48.
A. R. E. SINCLAIR 《African Journal of Ecology》1973,11(1):93-107
Censuses of buffalo and wildebeest in the Serengeti-Mara region have been carried out by various workers at intervals since 1958. The methods of these censuses, which normally employ a total count, are described and the errors inherent in such techniques are analysed in detail. After the appropriate corrections had been made the results showed that both populations have increased considerably over the years 1961–1971. 相似文献
49.
T. B. Lapirova V. R. Mikryakova A. S. Mavrin G. A. Yinogradova 《Journal of Evolutionary Biochemistry and Physiology》2000,36(1):47-51
The paper presents data on a change in lysozyme content in tissues of spleen, liver and heart in fry of the Lena River sturgeon
exposed to the presence of sublethal concentrations of Hg2+, Cd2+, and Cu2+ under conditions of chronic experiment. It has been shown that the lysozyme content in fish tissues varies and has a phasic
character. The amplitude of fluctuations of this parameter depends on the moment of sampling, nature of the toxicant, and
structural-functional organization of the studied organs. 相似文献
50.
(Z)- and (E)-4-amino-2-(trifluoromethyl)-2-butenoic acid (4 and 5, respectively) were synthesized and investigated as potential mechanism-based inactivators of gamma-aminobutyric acid aminotransferase (GABA-AT) in a continuing effort to map the active site of this enzyme. The core alpha-trifluoromethyl-alpha,beta-unsaturated ester moiety was prepared via a Reformatsky/reductive elimination coupling of the key intermediates tert-butyl 2,2-dichloro-3,3,3-trifluoropropionate and N,N-bis(tert-butoxy-carbonyl)glycinal. Both 4 and 5 inhibited GABA-AT in a time-dependent manner, but displayed non-pseudo-first-order inactivation kinetics; initially, the inactivation rate increased with time. Further investigation demonstrated that the actual inactivator is generated enzymatically from 4 or 5. This inactivating species is released from the active site prior to inactivation, and as a result, 4 and 5 cannot be defined as mechanism-based inactivators. Furthermore, 4 and 5 are alternate substrates for GABA-AT, transaminated by the enzyme with Km values of 0.74 and 20.5 mM, respectively. Transamination occurs approximately 276 and 305 times per inactivation event for 4 and 5, respectively. The enzyme also catalyzes the elimination of the fluoride ion from 4 and 5. A mechanism to account for these observations is proposed. 相似文献