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21.
James L Crainey Túllio RR da Silva Fernando Encinas Michel A Marín Ana Carolina P Vicente Sérgio LB Luz 《Memórias do Instituto Oswaldo Cruz》2016,111(1):79-81
We report here the first complete mitochondria genome of Onchocerca
volvulus from a focus outside of Africa. An O. volvulus
mitogenome from the Brazilian Amazonia focus was obtained using a combination of
high-throughput and Sanger sequencing technologies. Comparisons made between this
mitochondrial genome and publicly available mitochondrial sequences identified 46
variant nucleotide positions and suggested that our Brazilian mitogenome is more
closely related to Cameroon-origin mitochondria than West African-origin
mitochondria. As well as providing insights into the origins of Latin American
onchocerciasis, the Brazilian Amazonia focus mitogenome may also have value as an
epidemiological resource. 相似文献
22.
Hierarchical folding and reorganization of chromosomes are linked to transcriptional changes in cellular differentiation 下载免费PDF全文
Mariano Barbieri Benjamin L Moore Dorothee CA Kraemer Stuart Aitken Sheila Q Xie Kelly J Morris Masayoshi Itoh Hideya Kawaji Ines Jaeger Yoshihide Hayashizaki Piero Carninci Alistair RR Forrest The FANTOM Consortium Colin A Semple Josée Dostie Ana Pombo Mario Nicodemi 《Molecular systems biology》2015,11(12)
23.
24.
Marcia Berrêdo-Pinho Dario E Kalume Paloma R Correa Leonardo HF Gomes Melissa P Pereira Renata F da Silva Luiz RR Castello-Branco Wim M Degrave Leila Mendonça-Lima 《BMC microbiology》2011,11(1):80
Background
Bacille Calmette-Guerin (BCG) is currently the only available vaccine against tuberculosis (TB) and comprises a heterogeneous family of sub-strains with genotypic and phenotypic differences. The World Health Organization (WHO) affirms that the characterization of BCG sub-strains, both on genomic and proteomic levels, is crucial for a better comprehension of the vaccine. In addition, these studies can contribute in the development of a more efficient vaccine against TB. Here, we combine two-dimensional electrophoresis (2DE) and mass spectrometry to analyse the proteomic profile of culture filtrate proteins (CFPs) from M. bovis BCG Moreau, the Brazilian vaccine strain, comparing it to that of BCG Pasteur. CFPs are considered of great importance given their dominant immunogenicity and role in pathogenesis, being available for interaction with host cells since early infection. 相似文献25.
Alice Grison Silvia Zucchelli Alice Urzì Ilaria Zamparo Dejan Lazarevic Giovanni Pascarella Paola Roncaglia Alejandro Giorgetti Paula Garcia-Esparcia Christina Vlachouli Roberto Simone Francesca Persichetti Alistair RR Forrest Yoshihide Hayashizaki Paolo Carloni Isidro Ferrer Claudia Lodovichi Charles Plessy the FANTOM Consortium Piero Carninci Stefano Gustincich 《BMC genomics》2014,15(1)
Background
The mesencephalic dopaminergic (mDA) cell system is composed of two major groups of projecting cells in the Substantia Nigra (SN) (A9 neurons) and the Ventral Tegmental Area (VTA) (A10 cells). Selective degeneration of A9 neurons occurs in Parkinson’s disease (PD) while abnormal function of A10 cells has been linked to schizophrenia, attention deficit and addiction. The molecular basis that underlies selective vulnerability of A9 and A10 neurons is presently unknown.Results
By taking advantage of transgenic labeling, laser capture microdissection coupled to nano Cap-Analysis of Gene Expression (nanoCAGE) technology on isolated A9 and A10 cells, we found that a subset of Olfactory Receptors (OR)s is expressed in mDA neurons. Gene expression analysis was integrated with the FANTOM5 Helicos CAGE sequencing datasets, showing the presence of these ORs in selected tissues and brain areas outside of the olfactory epithelium. OR expression in the mesencephalon was validated by RT-PCR and in situ hybridization. By screening 16 potential ligands on 5 mDA ORs recombinantly expressed in an heterologous in vitro system, we identified carvone enantiomers as agonists at Olfr287 and able to evoke an intracellular Ca2+ increase in solitary mDA neurons. ORs were found expressed in human SN and down-regulated in PD post mortem brains.Conclusions
Our study indicates that mDA neurons express ORs and respond to odor-like molecules providing new opportunities for pharmacological intervention in disease.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-729) contains supplementary material, which is available to authorized users. 相似文献26.
Rapid, sequential changes in surface morphology of PC12 pheochromocytoma cells in response to nerve growth factor 总被引:20,自引:17,他引:20 下载免费PDF全文
The effect of nerve growth factor (NGF), a substance that promotes the differentiation and maintenance of certain neurons, was studied via scanning electron microscopy utilizing the PC12 clonal NGF-responsive pheochromocytoma cell line. After 2-4 d of exposure to NGF, these cells acquire many of the properties of normal sympathic neurons. However, by phase microscopy, no changes are discernible within the first 12-18 h. Since the primary NGF receptor appears to be a membrane receptor, it seemed likely that some of the initial responses to the factor may be surface related. PC12 cells maintained without NGF are round to ovoid and have numerous microvilli and small blebs. After the addition of NGF, there is a rapidly initiated sequential change in the cell surface. Ruffles appear over the dorsal surface of the cells with 1 min, become prominent by 3 min, and almost disappear by 7 min. Microvilli, conversely, disappear as the dorsal ruffles become prominent. Ruffles are seen at the the periphery of cell at 3 min, are prominent on most of the cells by 7 min and are gone by 15 min. The surface remains smooth from 15 min until 45 min when large blebs appear. The large blebs are present on most cells at 2 h and are gone by 4 h. The surface remains relatively smooth until 6-7 h of NGF treatment, when microvilli reappear as small knobs. These microvilli increase in both number and length to cover the cell surface by 10 h. These changes were not observed with other basic proteins, with α-bungarotoxin (which binds specifically to PC12 membranes), and were not affected by an RNA synthesis inhibitor that blocks initiation of neurite outgrowth. Changes in the cell surface architecture appear to be among the earlist NGF responses yet detected and may represent or reflect primary events in the mechanism of the factor’s action. 相似文献
27.
Audrey B. Condren Anil Kumar Pradeep Mettu Katharine J. Liang Lian Zhao Jen-yue Tsai Robert N. Fariss Wai T. Wong 《PloS one》2013,8(1)
Objective
Perivascular mural cells of the choroid have been implicated in physiological functioning as well as in retinal disease pathogenesis. However details regarding their form and function are not well understood. We aim to characterize choroidal mural cells in the adult mouse choroid in terms of their distribution and morphology, and correlate these to their contractile behavior.Methods
Sclerochoroidal flat-mounted explants were prepared from albino transgenic mice in which the α-smooth muscle actin (α-SMA) promoter drives the expression of green fluorescent protein (GFP). α-SMA-expressing smooth muscle cells and pericytes in the living choroid were thereby rendered fluorescent and imaged with confocal microscopy and live-cell imaging in situ.Results
Choroidal perivascular mural cells demonstrate significant diversity in terms of their distribution and morphology at different levels of the vasculature. They range from densely-packed circumferentially-oriented cells that provide complete vascular coverage in primary arteries to widely-spaced stellate-shaped cells that are distributed sparsely over terminal arterioles. Mural cells at each level are immunopositive for contractile proteins α-SMA and desmin and demonstrate vasoconstrictory contractile movements in response to endothelin-1 and the calcium ionophore, A23187, and vasodilation in response to the calcium chelator, BAPTA. The prominence of vasoregulatory contractile responses varies with mural cell morphology and density, and is greater in vessels with dense coverage of mural cells with circumferential cellular morphologies. In the choriocapillaris, pericytes demonstrate a sparse, horizontal distribution and are selectively distributed only to the scleral surface of the choriocapillaris.Conclusions
Diversity and regional specialization of perivascular mural cells may subserve varying requirements for vasoregulation in the choroid. The model of the α-SMA-GFP transgenic albino mouse provides a useful and intact system for the morphological and functional study of choroidal mural cells. 相似文献28.
29.
Alexia?Hermanny M?Valeria?Bahamondes Francisco?Fazano Nadia?M?Marchi Maria?Elena?Ortiz Maria?Heloisa?RR?Genghini Horacio?B?Croxatto Luis?BahamondesEmail author 《Reproductive biology and endocrinology : RB&E》2012,10(1):8
Background
The mechanism of action of levonorgestrel (LNG) as emergency contraception (EC) remains a subject of debate and its effect on sperm function has been only partially explained. The aim of this study was to assess whether LNG at a similar dose to those found in serum following oral intake for EC could affect spermatozoa when exposed to human fallopian tubes in vitro. 相似文献30.
Cloonan N Brown MK Steptoe AL Wani S Chan WL Forrest AR Kolle G Gabrielli B Grimmond SM 《Genome biology》2008,9(8):R127-14