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61.
62.
Charles G. Messing 《Invertebrate Biology》2003,122(3):280-292
Abstract. Examination of the living bathyal feather star, Atelecrinus, using light and scanning electron microscopy reveals a series of morphological features, some apparently unique within the Crinoidea, which forces a reassessment of the relationship of the genus to other living feather stars. In the cavernous centrodorsal cavity, interradial buttresses end with a shallow oral concavity or deep pit. Two adjacent axils each bear a large distolateral calcareous process of unknown function that resembles a shoe with a deeply ridged sole. These processes project into the visceral mass with their “soles” apposed—the only known case among living crinoids in which a skeletal feature appears asymmetrically on two of five rays. Articular facets of distal brachials bear one distally‐projecting, distal muscular fossa that overlaps a recumbent muscular fossa on the succeeding ossicle. Apposed projecting and recumbent fossae alternate sides on successive ossicles. The arm terminates in a long filament that lacks pinnules. Finally, modified ambulacral lappets resembling sessile pedicellariae but unsupported by skeletal plates flank the ambulacral grooves along the middle and proximal arms. Two of the four genera in the Atelecrinidae, extant monotypic Sibogacrinus anomalus and Cretaceous Jaekelometra, are removed from the family and treated as incertae sedis. Unique cirrus sockets are found in Atelecrinus and Atopocrinus sibogae (the only other remaining atelecrinid); members of Atopocrinus share unique ray morphology with those of the Pentametrocrinidae, and uniquely modified distal brachials are shared by individuals of Atelecrinus and Pentametrocrinus varians. However, the relationship of Atelecrinus to other feather stars remains unclear. The genus may represent a highly derived, paedomorphic comatulid as suggested by its unmetamorphosed basal ring and lack of proximal pinnules, or a non‐comatulid distinguished by its cirrus sockets, skeletal processes and pedicellaria‐like features. Robust answers require fresh material suitable for ultra‐structural and molecular analyses. 相似文献
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The maize high-lysine mutant opaque7 is defective in an acyl-CoA synthetase-like protein 总被引:2,自引:0,他引:2
Maize (Zea mays) has a large class of seed mutants with opaque or nonvitreous endosperms that could improve the nutritional quality of our food supply. The phenotype of some of them appears to be linked to the improper formation of protein bodies (PBs) where zein storage proteins are deposited. Although a number of genes affecting endosperm vitreousness have been isolated, it has been difficult to clone opaque7 (o7), mainly because of its low penetrance in many genetic backgrounds. The o7-reference (o7-ref) mutant arose spontaneously in a W22 inbred, but is poorly expressed in other lines. We report here the isolation of o7 with a combination of map-based cloning and transposon tagging. We first identified an o7 candidate gene by map-based cloning. The putative o7-ref allele has a 12-bp in-frame deletion of codons 350-353 in a 528-codon-long acyl-CoA synthetase-like gene (ACS). We then confirmed this candidate gene by generating another mutant allele from a transposon-tagging experiment using the Activator/Dissociation (Ac/Ds) system in a W22 background. The second allele, isolated from ~1 million gametes, presented a 2-kb Ds insertion that resembles the single Ds component of double-Ds, McClintock's original Dissociation element, at codon 496 of the ACS gene. PBs exhibited striking membrane invaginations in the o7-ref allele and a severe number reduction in the Ds-insertion mutant, respectively. We propose a model in which the ACS enzyme plays a key role in membrane biogenesis, by taking part in protein acylation, and that altered PBs render the seed nonvitreous. 相似文献
65.
Giovanni Benelli Nickolas G. Kavallieratos Elisa Donati Margherita Mencattelli Gabriella Bonsignori Cesare Stefanini Angelo Canale Russell H. Messing 《BioControl》2014,59(5):487-500
Aphidius colemani Viereck (Hymenoptera: Braconidae) is a pan-tropical broadly oligophagous endoparasitoid of many aphids of economic importance, including Aphis gossypii Glover and Myzus persicae (Sulzer). While the trophic interactions occurring among A. colemani and its hosts have been extensively studied, little is known about the male- and female-borne cues that guide mating dynamics. Male wing fanning has been found to play a key role in A. colemani courtship, as successful mounting of females without initial wing fanning has never been observed. In this research, we analyzed wing fanning performance and mating ability of males from three different strains of A. colemani: wasps commercially mass-reared on A. gossypii, wild wasps from parasitized A. gossypii, and wild wasps from parasitized Aphis nerii Boyer de Fonscolombe. Results showed that virgin females did not rely on particular male fanning features during mate choice. Moreover, when A. colemani individuals developed on A. gossypii, no major differences were detected in courtship and mating ability between field collected and mass-reared wasps. In contrast, courtship performance and mating success varied between wild A. colemani males reared on different hosts, with those developing on A. nerii having lower quality wing fanning performance during the mounting attempt phase, and reduced ability to compete for females with other males reared on A. gossypii. 相似文献
66.
Ray Gervacio F. Blanco Joseph Califano Barbara Messing Jeremy Richmon Jia Liu Harry Quon Geoffrey Neuner John Saunders Patrick K. Ha Sheila Sheth Maura Gillison Carole Fakhry 《PloS one》2014,9(1)
Background
Base of tongue (BOT) is a difficult subsite to examine clinically and radiographically. Yet, anatomic delineation of the primary tumor site, its extension to adjacent sites or across midline, and endophytic vs. exophytic extent are important characteristics for staging and treatment planning. We hypothesized that ultrasound could be used to visualize and describe BOT tumors.Methods
Transcervical ultrasound was performed using a standardized protocol in cases and controls. Cases had suspected or confirmed BOT malignancy. Controls were healthy individuals without known malignancy.Results
100% of BOT tumors were visualized. On ultrasound BOT tumors were hypoechoic (90.9%) with irregular margins (95.5%). Ultrasound could be used to characterize adjacent site involvement, midline extent, and endophytic extent, and visualize the lingual artery. No tumors were suspected for controls.Conclusions
Ultrasonography can be used to transcervically visualize BOT tumors and provides clinically relevant characteristics that may not otherwise be appreciable. 相似文献67.
Ladybirds are a hot-spot for the invasion of male-killing bacteria. These maternally inherited endosymbionts cause the death of male host embryos, to the benefit of female sibling hosts and the bacteria that they contain. Previous studies have shown that high temperatures can eradicate male-killers from ladybirds, leaving the host free from infection. Here we report the discovery of two maternally inherited sex ratio distorters in populations of a coccinellid, Coccinella undecimpunctata, from a hot lowland region of the Middle East. DNA sequence analysis indicates that the male killing is the result of infection by Wolbachia, that the trait is tetracycline sensitive, and that two distinct strains of Wolbachia co-occur within one beetle population. We discuss the implications of these findings for theories of male-killing and suggest avenues for future field-work on this system. 相似文献
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Exposure to ethanol for several days increases the expression of dihydropyridine-sensitive, voltage-dependent Ca2+ channels in brain and in the neural cell line PC12. Since protein phosphorylation is a major mechanism by which ion channels are regulated, we used protein kinase inhibitors to investigate whether ethanol-induced up-regulation of Ca2+ channels involves activation of a protein kinase. Sphingosine and polymixin B, which inhibit protein kinase C and calmodulin-dependent kinases, prevented the enhancement of 45Ca2+ uptake induced by exposure of PC12 cells to ethanol for 4 days. In addition, sphingosine blocked the ability of ethanol to increase the number of [3H]dihydropyridine binding sites in PC12 cell membranes. Sphingosine's effect was prevented by simultaneous exposure to phorbol 12,13-dibutyrate, a potent activator of protein kinase C. Therefore, protein kinase C appears to be involved in the up-regulation of dihydropyridine-sensitive Ca2+ channels during prolonged exposure to ethanol. 相似文献