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Corrales IF Cortés JA Mesa ML Zamora G 《Biomédica : revista del Instituto Nacional de Salud》2003,23(2):202-207
BCG vaccine has been used for nearly 100 years in the prevention of tuberculosis. The case of a 13 month-old girl vaccinated as a newborn with BCG is described as presenting a sternal mass with associated periostic erosion. The mass was resected, and histopathological examination revealed a chronic inflammatory reaction with caseous granulomas, and extension to bone and skin. PCR amplifications using specific primers for Mycobacterium tuberculosis on paraffin-embedded tissue were negative. BCG vaccination at birth, the histological appearance and the absence of M. tuberculosis DNA in the resected tissue indicated that osteomyelitis and scrofuloderma were a consequence of BCG. Osteomyelitis is a rarely occurring adverse effect of BCG vaccination, more commonly seen in immunosuppressed patients. In the patient described above, no symptoms of immunodeficiency were seen, however. 相似文献
14.
Sanchez-Berrondo J Mesa P Ibarra A Martínez-Jiménez MI Blanco L Méndez J Boskovic J Montoya G 《Nucleic acids research》2012,40(3):1366-1380
DNA replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. One of them is the replicative helicase, which is required for initiation and elongation phases. A MCM helicase found as a prophage in the genome of Bacillus cereus is fused with a primase domain constituting an integrative arrangement of two essential activities for replication. We have isolated this helicase-primase complex (BcMCM) showing that it can bind DNA and displays not only helicase and primase but also DNA polymerase activity. Using single-particle electron microscopy and 3D reconstruction, we obtained structures of BcMCM using ATPγS or ADP in the absence and presence of DNA. The complex depicts the typical hexameric ring shape. The dissection of the unwinding mechanism using site-directed mutagenesis in the Walker A, Walker B, arginine finger and the helicase channels, suggests that the BcMCM complex unwinds DNA following the extrusion model similarly to the E1 helicase from papillomavirus. 相似文献
15.
Emile CL. Marnette Harm Houweling Herman Van Dam Jan Willem Erisman 《Biogeochemistry》1993,23(2):119-144
The chemical composition of surface waters of two Dutch moorland pools and of incident precipitation, was monitored from 1982
to 1990. For this period, sulfur and water budgets were calculated using a hydrochemical model developed for well-mixed non-stratifying
lakes. Total atmospheric deposition of S decreased significantly after 1986 at both locations. A model describing the sulfur
budget in terms of input, output and reduction/oxidation processes predicted a fast decrease of pool water SO4
2− concentrations after a decrease of atmospheric input. However, SO4
2− concentrations in the surface water was lowered only slightly or remained constant. Apparently a source within the lake caused
the unexpectedly high SO4
2− concentrations. The possible supply of SO4
2− from the sediment through regulation by (K-)Al-SO4 containing minerals or desorption of SO4
2− from positively charged surfaces in the sediment was evaluated. Solubility calculations of pore water with respect to alunite,
basaluminite and jurbanite indicated that SO4
2− concentration was not regulated by these minerals. It is suggested here (1) that desorption of SO4
2− from peaty sediments may account for the estimated SO4
2− supply provided that the adsorption complex is periodically recharged by partial oxidation of the upper bottom sediments
and (2) that because of exposure of a part of the pool bottom to the atmosphere during dry summers and subsequent oxidation
of reduced S, the amount of SO4
2− may be provided which complements the decreasing depositional SO4
2− input. In future research these two mechanisms need to be investigated. 相似文献
16.
MORAIS PAULO AMORIM ANTÓNIO VIEIRA DA SILVA CLÁUDIA RIBEIRO TERESA COSTA SANTOS JORGE AFONSO COSTA HELOÍSA 《Journal of genetics》2015,94(3):509-512
Journal of Genetics - 相似文献
17.
Mario La Mesa 《Polar Biology》2007,30(10):1219-1226
Juvenile Antarctic toothfish (Dissostichus mawsoni) collected off the South Shetland Islands (CCAMLR Subarea 48.1) were aged by counting the presumed daily increments in the
sagittal otoliths. The timing and position of the first annulus was examined for each individual fish from 10 to 13 cm TL.
Under reflected light at low magnification, the otolith sections exhibited a wide opaque nucleus, with no evidence of deposition
of the first translucent zone. The average measurement of ventral and dorsal radii of these sections were, respectively, 603
and 768 μm, consistently less than the measurements done in previously estimated 1 year old fish caught in the same area.
All specimens aged in the present study were approximately 1 year old fish, with an age range between 365 and 431 days, suggesting
a significant underestimation of age in the previous studies. The value of ageing precision indices calculated was relatively
low, supporting the reliability of the reading technique applied. Two checks were easily identified in the core of all otolith
sections, interpreted tentatively as hatching and first exogenous feeding checks. Back-calculating from the time of capture,
the hatching period of D. mawsoni was estimated to occur throughout the summer season, from November to February. In conclusion, the delay in timing of deposition
of the first translucent zone, which occurs in the second winter from hatching, should be taken into account in future studies
on ageing Antarctic toothfish, as well as in the stock assessment models based on length at age data. 相似文献
18.
In comparison with other bathydraconids, all species of the genus Bathydraco are poorly known from an ecological perspective. The diet of juvenile Bathydraco marri Norman, 1938 was studied for the first time in specimens collected in the southwestern Ross Sea during summer 1998. Fish
were collected in a single otter trawl catch at 330–340 m depth. The stomach content analysis showed that this species fed
exclusively on crustaceans. Overall, 20 prey taxa were identified to genus or species level. Mysids, amphipods and copepods
were the most important prey in decreasing order of importance. Other prey, such as Euphausia superba, isopods and tanaids were eaten occasionally and in very small amounts. A multivariate analysis was applied to feeding data
to assess ontogenetic or sex-related changes in diet. No difference was detected between sexes, whereas diet of small and
large fish differed in some degree. An ontogenetic shift from small and pelagic crustaceans such as copepods to benthic–benthopelagic
prey such as amphipods and mysids was observed. Relating present results with published data on physiological characteristics
of B. marri, it was possible to infer their feeding behaviour and mode of life. Like other bathydraconids, this species appeared to be
an inactive and sluggish fish, which relied on more or less motile benthic or epibenthic prey adopting a “sit and wait” feeding
strategy. On the other hand, smaller fish seem to be more active, feeding also on pelagic prey such as copepods that can be
seasonally abundant, thus reducing the intraspecific competition for food. 相似文献
19.
MARTIN CLÉMENT CAROLINE CHAMBERLAND JACQUELINE PÉRODIN RICHARD LEDUC GAÉTAN GUILLEMETTE EMANUEL ESCHER 《Journal of receptor and signal transduction research》2013,33(5-6):417-433
Several models of activation mechanisms were proposed for G protein-coupled receptors (GPCRs), yet no direct methods exist for their elucidation. The availability of constitutively active mutants has given an opportunity to study active receptor conformations within acceptable limits using models such as the angiotensin II type 1 (AT1)1 receptor mutant N111G-hAT1 which displays an important constitutive activity. Recently, by using methionine proximity assay, we showed for the hAT1 receptor that TMD III, VI, and VII form the ligand-binding pocket of the C-terminal amino acid of an antagonistic AngII analogue. In the present contribution, we investigated whether the same residues would also constitute the ligand-binding contacts in constitutively activated mutant (CAM) receptors. For this purpose, the same Met mutagenesis strategy was carried out on the N111G double mutants. Analysis of 43 receptors mutants in the N111G-hAT1 series, photolabeled and CNBr digested, showed that there were only subtle structural changes between the wt-receptor and its constitutively active form. 相似文献
20.
Oylum Erkus Victor CL de Jager Maciej Spus Ingrid J van Alen-Boerrigter Irma MH van Rijswijck Lucie Hazelwood Patrick WM Janssen Sacha AFT van Hijum Michiel Kleerebezem Eddy J Smid 《The ISME journal》2013,7(11):2126-2136
Maintenance of a high degree of biodiversity in homogeneous environments is poorly understood. A complex cheese starter culture with a long history of use was characterized as a model system to study simple microbial communities. Eight distinct genetic lineages were identified, encompassing two species: Lactococcus lactis and Leuconostoc mesenteroides. The genetic lineages were found to be collections of strains with variable plasmid content and phage sensitivities. Kill-the-winner hypothesis explaining the suppression of the fittest strains by density-dependent phage predation was operational at the strain level. This prevents the eradication of entire genetic lineages from the community during propagation regimes (back-slopping), stabilizing the genetic heterogeneity in the starter culture against environmental uncertainty. 相似文献