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821.
Loren Merrill Stephen A. Tyndel Michael P. Ward Thomas J. Benson Jinelle H. Sperry 《Journal of Field Ornithology》2020,91(2):189-198
Researchers and managers have used the tendency of some species of birds to settle near conspecifics (i.e., conspecific attraction) to help establish or reestablish species of conservation concern in targeted habitats. However, most studies of conspecific attraction as a recruitment tool have been conducted with either migratory or colonial-breeding species, and less is known about the possible importance of conspecific attraction for resident species. In 2017 and 2018 in Louisiana, we examined the possible use of conspecific attraction as a management tool for Northern Bobwhites (Colinus virginianus), game birds that are year-round residents. We used an alternating split experimental design across years where Northern Bobwhite vocalizations were broadcast at half of our study sites during the first year of our study and the other half were controls. The following year, experimental sites became controls, and controls became experimental sites. We also assessed land cover at each site to determine if variation in habitat composition at small spatial scales influenced bobwhite presence. We detected six times more Northern Bobwhites at treatment sites (sites with playback) than control sites and found a positive association between the proportion of grass cover and bobwhite presence. These results suggest that a non-migratory, resident species may select breeding locations based, at least in part, on the presence of conspecifics, and that playback of their calls could be incorporated into management plans. Playing back conspecific calls in early spring when Northern Bobwhites are likely searching for breeding locations may facilitate colonization and allow individuals to find locations where managers are attempting to restore their populations. 相似文献
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Rheology of blood and flow in the microcirculation 总被引:3,自引:0,他引:3
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Zachari Turgeon Dawn White René Jørgensen Danielle Visschedyk Robert J. Fieldhouse Dev Mangroo & A. Rod Merrill 《FEMS microbiology letters》2009,300(1):97-106
The emergence of bacterial antibiotic resistance poses a significant challenge in the pursuit of novel therapeutics, making new strategies for drug discovery imperative. We have developed a yeast growth-defect phenotypic screen to help solve this current dilemma. This approach facilitates the identification and characterization of a new diphtheria toxin (DT) group, ADP-ribosyltransferase toxins from pathogenic bacteria. In addition, this assay utilizes Saccharomyces cerevisiae , a reliable model for bacterial toxin expression, to streamline the identification and characterization of new inhibitors against this group of bacterial toxins that may be useful for antimicrobial therapies. We show that a mutant of the elongation factor 2 target protein in yeast, G701R, confers resistance to all DT group toxins and recovers the growth-defect phenotype in yeast. We also demonstrate the ability of a potent small-molecule toxin inhibitor, 1,8-naphthalimide (NAP), to alleviate the growth defect caused by toxin expression in yeast. Moreover, we determined the crystal structure of the NAP inhibitor–toxin complex at near-atomic resolution to provide insight into the inhibitory mechanism. Finally, the NAP inhibitor shows therapeutic protective effects against toxin invasion of mammalian cells, including human lung cells. 相似文献
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Kathy Darragh Anna Orteu Daniella Black Kelsey J. R. P. Byers Daiane Szczerbowski Ian A. Warren Pasi Rastas Ana Pinharanda John W. Davey Sylvia Fernanda Garza Diana Abondano Almeida Richard M. Merrill W. Owen McMillan Stefan Schulz Chris D. Jiggins 《PLoS biology》2021,19(1)
Plants and insects often use the same compounds for chemical communication, but not much is known about the genetics of convergent evolution of chemical signals. The terpene (E)-β-ocimene is a common component of floral scent and is also used by the butterfly Heliconius melpomene as an anti-aphrodisiac pheromone. While the biosynthesis of terpenes has been described in plants and microorganisms, few terpene synthases (TPSs) have been identified in insects. Here, we study the recent divergence of 2 species, H. melpomene and Heliconius cydno, which differ in the presence of (E)-β-ocimene; combining linkage mapping, gene expression, and functional analyses, we identify 2 novel TPSs. Furthermore, we demonstrate that one, HmelOS, is able to synthesise (E)-β-ocimene in vitro. We find no evidence for TPS activity in HcydOS (HmelOS ortholog of H. cydno), suggesting that the loss of (E)-β-ocimene in this species is the result of coding, not regulatory, differences. The TPS enzymes we discovered are unrelated to previously described plant and insect TPSs, demonstrating that chemical convergence has independent evolutionary origins.Plants and insects often use the same compounds for chemical communication, but little is known about the convergent evolution of such chemical signals. This study identifies a novel terpene synthase involved in production of an anti-aphrodisiac pheromone by the butterfly Heliconius melpomene. This enzyme is unrelated to other insect terpene synthases, providing evidence that the ability to synthesise terpenes has arisen multiple times independently within the insects. 相似文献
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