Phytohemagglutinin isolectin stimulation of glucose utilization by lymphocytes

Normal human lymphocytes were incubated with purified $\text{Phaseolus vulgaris}$ phytohemagglutinin isolectins L4 and E4 and the resulting tritiated thymidine incorporation and glucose utilization were measured. Both isolectins stimulated tritiated thymidine incorporation and glucose utilization, but L4 was at least 30 fold more potent. The dose response of glucose utilization parallelled the dose response of tritiated thymidine incorporation produced by the isolectins. Glucose supplementation of the stimulated cultures did not affect the kinetics or time course of tritiated thymidine incorporation.