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51.
Herbivory differentially alters plant litter dynamics of evergreen and deciduous trees 总被引:5,自引:0,他引:5
Here we propose that herbivore-induced changes in leaf litter quality can modify aboveground litterfall dynamics differentially in evergreen and deciduous trees. Because aboveground plant litterfall is an important source of nutrients in terrestrial ecosystems, any factor that alters plant litter quality can have large "afterlife" effects on the decomposition rate of that litter and the subsequent rate of nutrient release. Two contrasting patterns emerge from the literature and are corroborated by our two experimental case studies. First, in evergreens, herbivory commonly results in premature leaf abscission, improved litter "quality" and an acceleration of litter decomposition. Second, in deciduous trees, herbivory commonly results in the induction of secondary compounds that decelerates decomposition. We argue that these broad patterns reflect predictable differential responses to herbivores that can have important consequences for terrestrial nutrient cycling and productivity and that warrant more attention in the literature. 相似文献
52.
Five different concentrations (100, 250, 500, 1000 and 2000 μg/L of aflatoxin B1 were found to be inhibitory to seed germination and seedling growth (root and shoot lengths) of mustard seeds (variety Pusa
bold). These also lowered the levels of chlorophyll and carotenoids in the emerging leaves during seedling growth. The inhibitory
effect was correlated with the concentration of applied toxin. 相似文献
53.
54.
Invertebrate activity under snow in deciduous woods 总被引:1,自引:0,他引:1
Pitfall traps caught large numbers of representatives of 44 families of invertebrates just before snow fall and under the snow. Some groups of ectotherms were most active in late autumn and late winter, the activity of some increased throughout the winter and some showed no major change although subnivean temperatures were usually near 0°C. The most commonly trapped groups were: phalangids, collembolans, diplopods, coleopterans, dipterans, arachnids, gastropods and chilopods. 相似文献
55.
A pre-aspartate-specific protease from human leukocytes that cleaves pro-interleukin-1 beta 总被引:8,自引:0,他引:8
R A Black S R Kronheim J E Merriam C J March T P Hopp 《The Journal of biological chemistry》1989,264(10):5323-5326
Interleukin-1 beta is a 17.4-kilodalton hormone derived from a 33-kilodalton inactive precursor produced by monocytes. We used the precursor as a substrate to detect proteolytic activities in peripheral blood mono-nuclear cell-conditioned medium that might be involved in interleukin-1 beta processing. We found that the conditioned medium, following passage through DEAE-Sephacel, generates a biologically active fragment from the precursor that runs slightly higher than the mature hormone in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The responsible activity behaved as a single protein in ion exchange chromatography. It was completely inhibited by metal ion chelators and not by inhibitors of serine, cysteine, or aspartate proteases, and it was dependent on both calcium (or magnesium) and zinc. The enzyme was not inhibited by three substrate-based metalloprotease inhibitors, phosphoramidon, benzyloxycarbonyl-Gly-Leu-NH2, and N-(2-carboxy-3-phenylpropionyl)-Leu. NH2-terminal sequence analysis showed that cleavage of the precursor occurred between a histidine and an aspartate residue, and digestion of synthetic peptides indicated that the protease is specific for pre-aspartate cleavages. 相似文献
56.
The Association of d-Ribulose- 1,5-Bisphosphate Carboxylase/Oxygenase with Phosphoribulokinase 总被引:1,自引:0,他引:1 下载免费PDF全文
When Ribulose- 1,5-bisphosphate carboxylase/oxygenase was purified from spinach leaves (Spinacia oleracea) using precipitation with polyethylene glycol and MgCl2 followed by DEAE cellulose chromatography, 75% of phosphoribulokinase and 7% of phosphoriboisomerase activities copurified with ribulose- 1,5-bisphosphate carboxylase/oxygenase. This enzyme preparation showed ribose-5-phosphate and ribulose-5-phosphate dependent carboxylase and oxygenase activities which were nearly equivalent to its corresponding ribulose- 1,5-bisphosphate dependent activity. The ribose-5-phosphate and ribulose-5-phosphate dependent reaction rates were stable and linear for much longer time periods than the ribulose- 1,5-bisphosphate dependent rates. When sucrose gradients were used to purify ribulose- 1,5-bisphosphate carboxylase/oxygenase from crude stromal extracts, phosphoribulokinase was found to cosediment with ribulose- 1,5-bisphosphate carboxylase. Under these conditions most of the phosphoriboisomerase activity remained with the slower sedimenting proteins. Ammonium sulfate precipitation resulted in separation of the ribulose- 1,5-bisphosphate carboxylase peak from phosphoribulokinase peak. Crude extracts of peas Pisum sativum and spinach contained 0.725 to 0.730 milligram of phosphoribulokinase per milligram of chlorophyll, respectively, based on an enzyme-linked immunosorbent assay. 相似文献
57.
Richard S. Morrison Robert R. Brooks Roger D. Reeves François Malaisse Paul Horowitz Michael Aronson George R. Merriam 《Phytochemistry》1981,20(3):455-458
Copper and cobalt were determined in tissue extracts of five metallophytes from Shaba Province, Zaïre. About 4O% of the cobalt and copper was extractable into deionized water and a further 4O% was extractable in 0.2M hydrochloric acid. It was concluded that copper and cobalt are bound to several different ligands instead of a single ligand as in the case of nickel hyperaccumulators. Proton microprobe studies on the cobalt accumulator Haumaniastrum robertii showed a strong inverse correlation between the spatial distributions of cobalt and potassium, as well as a direct relationship between cobalt and calcium. It is suggested that some of the cobalt may be immobilized with calcium in oxalate crystals. 相似文献
58.
The Relative Concentration of Solids in the Nucleolus, Nucleus, and Cytoplasm of the Developing Nerve Cell of the Chick 下载免费PDF全文
Growing and differentiating nerve cells of the fifth cranial ganglion of the chick embryo were studied by several means. During the period of 70 hours to 11 days of incubation (Hamburger-Hamilton stages 19 to 37) average cell mass increased more than 4.5 times while cells changed from relatively undifferentiated neuroblasts to morphologically characteristic nerve cells with long processes. By making simplifying assumptions about thickness of nucleus and nucleolus, relative to cytoplasmic thickness, it was possible to calculate solute concentration of nucleus and nucleolus relative to that of the cytoplasm from measurements of optical retardations through living cells. Differences in relative solute concentration were observed in nucleolus, cytoplasm, and nucleoplasm in the approximate ratio 1.2:1.0:0.8, respectively. The ratio remained essentially constant during the growth period examined despite the fact that the cell components grow at markedly different rates. This suggests that solid concentrations are physical characteristics of nucleus, nucleolus, and cytoplasm which are maintained even during rapid growth and differentiation. By cytochemical means it was demonstrated that mass increase in the nucleus is not associated with increase in deoxyribonucleic acid. Both ribonucleic acid and protein are in greater concentration in nucleolus and cytoplasm than in the nucleoplasm. Electron microscopy shows interruptions in the nuclear envelope as well as an approximately even distribution of electron density in nucleus and cytoplasm. It is pointed out that consistent differences in solid concentration can exist on either side of the nuclear envelope even though it contains "pores." Implications of these data are discussed. 相似文献
59.
60.
The Drosophila gene tailless is expressed at the embryonic termini and is a member of the steroid receptor superfamily 总被引:29,自引:0,他引:29
F Pignoni R M Baldarelli E Steingrímsson R J Diaz A Patapoutian J R Merriam J A Lengyel 《Cell》1990,62(1):151-163
The zygotically active tailless (tll) gene plays a key role in the establishment of nonmetameric domains at the anterior and posterior poles of the Drosophila embryo. We have cloned the tll gene and show that it encodes a protein with striking similarity to steroid hormone receptors in both the DNA binding "finger" and ligand binding domains. tll RNA is initially expressed in embryos in two mirror-image symmetrical domains; this pattern then quickly resolves into a pattern consistent with the mutant phenotype: a posterior cap and an anterior dorsal stripe. That the tll gene may also play a role in the nervous system is suggested by its strong expression in the forming brain and transient expression in the peripheral nervous system. 相似文献