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231.
Arnulfo Medina Celia A. Harvey Dalia Sánchez Merlo Sergio Vílchez Blas Hernández 《Biotropica》2007,39(1):120-128
Although agriculture dominates much of Central America, little is known about the bat assemblages that occur within agricultural landscapes and how bats use different types of tree cover within these landscapes. Using mist-nets and a mark-recapture protocol, we compared bat diversity and movement across six types of tree cover within an agricultural landscape in central Nicaragua. The tree cover types surveyed included secondary forests, riparian forests, forest fallows, live fences, pastures with high tree cover and pastures with low tree cover. We captured a total of 3084 bats of 39 species, including two new species records for the country ( Lonchorhina aurita and Molossops greenhalli ). Of these, 2970 bats and 27 species were in the Phyllostomidae family. There were significant differences in mean species density, abundance and evenness of phyllostomid bats across the different types of tree cover, but not in bat diversity. Riparian forests had the highest mean species density and bat abundance per plot. In contrast, mean bat abundance and species density were lowest in pastures with low tree cover. Of the 1947 phyllostomid bats marked, a total of 64 bats of eight species were recaptured. The average linear distance between extra-site recaptures was 2227 m (± 228 SE) and the maximum distance was 10.6 km. Bats were recorded moving between almost all types of tree cover, and especially to and from riparian forests. Our study suggests that agricultural landscapes retaining a heterogeneous tree cover may maintain a diverse bat assemblage, and that bats visit and use a variety of tree cover types within the agricultural matrix. 相似文献
232.
Body measurements, hematology, and serum chemistry values of the adult Cebus apella monkey 总被引:1,自引:1,他引:0
J M Rosner A Schinini T Rovira R Merlo R Bestard M Maldonado 《Journal of medical primatology》1986,15(4):295-302
Body measurements, hematology, and serum chemistry values were studied in 40 captured male and female Cebus apella monkeys. Some significant dimorphism with male predominance was found. Significant differences were also found for hemoglobin and red cell volume between males and females. Differential white blood cell counts indicated a marked predominance of lymphocytes and high values of gamma globulin in both sexes. 相似文献
233.
G. Lenaz Carla Bovina Cinzia Castelluccio Marika Cavazzoni E. Estornell Romana Fato J. R. Huertas Milena Merlo Pich F. Pallotti Giovanna Parenti Castelli Hana Rauchova 《Protoplasma》1995,184(1-4):50-62
Summary In the mitochondrial respiratory chain, coenzyme Q acts in different ways. A diffusable coenzyme Q pool as a common substrate-like intermediate links the low-potential complexes with complex III. Its diffusion in the lipids is not rate-limiting for electron transfer, but its content is not saturating for maximal rate of NADH oxidation. Protein-bound coenzyme Q is involved in energy conservation, and may be part of enzyme supercomplexes, as in succinate cytochromec reductase. The reason for lack of kinetic saturation of the respiratory chain by quinone concentration is in the low extent of solubility of monomeric coenzyme Q in the membrane lipids. Assays of respiratory enzymes are performed using water soluble coenzyme Q homologs and analogs; several problems exist in using oxidized quinones as acceptors of coenzyme Q reductases. In particular, for complex I no acceptor appears to favorably substitute the endogenous quinone. In addition, quinone reduction sites in complex III compete with the sites in the dehydrogenases, particularly when using duroquinone. The different extent by which these sites operate when different donor substrates (NADH, succinate, glycerol-3-phosphate) are used is best explained by different exposure of the quinone acceptor sites in the dehydrogenases. 相似文献
234.
Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit 总被引:24,自引:0,他引:24
Peter Geigenberger Ralph Reimholz Michael Geiger Lucia Merlo Vittoria Canale Mark Stitt 《Planta》1997,201(4):502-518
To investigate the effect of water stress on carbon metabolism in growing potato tubers (Solanum tuberosum L.), freshly cut and washed discs were incubated in a range of mannitol concentrations corresponding to external water potential
between 0 and −1.2 MPa. (i) Incorporation of [14C]glucose into starch was inhibited in water-stressed discs, and labeling of sucrose was increased. High glucose overrode
the changes at low water stress (up to −0.5 MPa) but not at high water stress. (ii) Although [14C]sucrose uptake increased in water-stressed discs, less of the absorbed [14C]sucrose was metabolised. (iii) Analysis of the sucrose content of the discs confirmed that increasing water deficit leads
to a switch, from net sucrose degradation to net sucrose synthesis. (iv) In parallel incubations containing identical concentrations
of sugars but differing in which sugar was labeled, degradation of [14C]sucrose and labeling of sucrose from [14C]glucose and fructose was found at each mannitol concentration. This shows that there is a cycle of sucrose degradation and
resynthesis in these tuber discs. Increasing the extent of water stress changed the relation between sucrose breakdown and
sucrose synthesis, in favour of synthesis. (v) Analysis of metabolites showed a biphasic response to increasing water deficit.
Moderate water stress (0–200 mM mannitol) led to a decrease of the phosphorylated intermediates, especially 3-phosphoglycerate
(3PGA). The decrease of metabolites at moderate water stress was not seen when high concentrations of glucose were supplied
to the discs. More extreme water stress (300–500 mM mannitol) was accompanied by an accumulation of metabolites at low and
high glucose. (vi) Moderate water stress led to an activation of sucrose phosphate synthase (SPS) in discs, and in intact
tubers. The stimulation involved a change in the kinetic properties of SPS, and was blocked␣by protein phosphatase inhibitors.
(vii) The amount of ADP-glucose (ADPGlc) decreased when discs were incubated on 100 or 200 mM mannitol. There was a strong
correlation between the in vivo levels of ADPGlc and 3PGA when discs were subjected to moderate water stress, and when the
sugar supply was varied. (viii) The level of ADPGlc increased and starch synthesis was further inhibited when discs were incubated
in 300–500 mM mannitol. (ix) It is proposed that moderate water stress leads to an activation of SPS and stimulates sucrose
synthesis. The resulting decline of 3PGA leads to a partial inhibition of ADP-glucose pyrophosphorylase and starch synthesis.
More-extreme water stress leads to a further alteration of partitioning, because it inhibits the activities of one or more
of the enzymes involved in the terminal reactions of starch synthesis.
Received: 26 August 1996 / Accepted: 5 November 1996 相似文献
235.
236.
B Brusoni G Marchetti L Merlo G M Minoja L Ladelli R Lacchini C F Melloni T Longo 《Bollettino della Società italiana di biologia sperimentale》1979,55(17):1773-1779
Experiments were performed on 19 anaesthetized open-chest dog instrumented with polyethylene catheters inserted: into the aorta, in pulmonary artery and in left atrium and with an electromagnetic flow-transducer placed around the ascending aorta in order to record : systemic arterial and pulmonary pressures, mean left auricular pressure and phasic aortic flow. Heart rate, stroke volume, total systemic and pulmonary resistance, cardiac work were moreover calculated. Each dog was given intravenously by slow infusione : Dopamine (micrograms 5--10--20/kg/min/ 5 min), Isoproterenol (microgram 0.125--0.25--0.5/kg/min/5 min) and Norepinephrine (microgram 0.25--0.5--1 /kg/min/5 min). Results obtained on systemic hemodynamics agree with those reported by many other investigators. On pulmonary circulation : Isoproterenol, at the tested doses, elicited vasodilator effects, Norepinephrine increased total pulmonary resistance but not pulmonary vascular resistance, while Dopamine did not modify or slightly reduced vascular pulmonary tone. 相似文献
237.
Prof. Dr. J. Metz III M. Merlo H. Billich W. G. Forssmann 《Cell and tissue research》1978,186(2):227-240
Summary At least four types of endocrine-like cells have been detected histochemically in the mucosa of the human colon and rectum, i.e. argentaffin cells storing 5-hydroxytryptamine (5 HT) and non-argentaffin cells reacting with glucagon, somatostatin and bovine pancreatic peptide (BPP) antibodies. Ultrastructurally, four main types and three rare types of endocrine-like cells have been identified. Among the former cells were: (1) argentaffin EC1 cells, known to store 5 HT and substance P, (2) poorly argyrophil L cells, corresponding to the glucagon-immunoreactive cells storing enteroglucagon or glucagon-like immunoreactivity (GL1), (3) inconstantly argyrophil F-like cells, possibly corresponding to BPP-immunoreactive cells, and (4) fairly argyrophil H cells of unknown function. Rare D cells, corresponding to somatostatin cells, N cells, corresponding to neurotensin cells, and P cells, of unknown function, have been also found.Supported in part by the Italian National Research Council (Grant N. 76.01558.04) 相似文献
238.
Mice of the TO Swiss strain received diets containing different amounts of saturated or unsaturated fat throughout life. These diets produced characteristic changes in cardiac phospholipid fatty acid composition, but produced no significant differences in fatty acid composition of phospholipids from a crude membrane fraction of brain. When littermates of these animals were exposed to ethanol vapour in an inhalation chamber it was observed that mice which had received a diet high in saturated fat lost the righting reflex at an estimated concentration of ethanol in blood higher than that required for mice receiving a control diet, or a diet rich in polyunsaturated fat. Analysis of the brain membrane fraction from those animals which had received ethanol revealed that mice receiving the highly saturated fat diet now had a significantly greater proportion of saturated fatty acids in brain membrane phospholipids. These results are discussed in relation to the hypothesis that brain membrane lipid composition may influence the behavioural response to ethanol. 相似文献
239.
Partial purification from potato tubers of three fructokinases and three hexokinases which show differing organ and developmental specificity 总被引:8,自引:0,他引:8
A combination of chromatography on DE-52 cellulose, Cibacron Blue agarose, Mono Q anion exchanger and gel filtration was used to resolve different hexose-phosphorylating enzymes from growing sink potato tubers (Solanum tuberosum L.). Three enzymes (fructokinases: FK1, FK2 and FK3) are active with fructose and inactive with glucose, and three (hexokinases: HK1, HK2 and HK3) are active with glucose but not with fructose. Elution from DE-52 columns showed that the relative abundance of the six activities changes, depending on the organ and on the developmental stage. FK1 and FK2 were present at high activities in tubers but at very low activity in leaves; conversely FK3 was present at very low activity in tubers but at high activity in leaves. During storage of potato tuber, and also during sprouting, there was a decrease of FK1 and FK2. In contrast, glucose-phosphorylating activity was very low in growing tubers. During storage and sprouting the activity of the glucose-phosphorylating enzymes rose, until they exceeded FK1 and FK2. This was due particularly to an increase of HK1, whereas HK2 declined relative to HK1, and HK3 was always negligible. These changes in the pattern of hexose-phosphorylating enzyme forms are compared with the changing metabolic fluxes and pools of hexose sugars in potato tubers. It is concluded that organ- and development-specific changes in the abundance of the various enzyme forms contribute to the regulation of hexose metabolism in the potato.Abbreviations DTT
dithiothreitol
- FK
fructokinase
- FPLC
fast protein liquid chromatography
- HK
hexokinase
- Susy
sucrose synthase
- UDPGlc
uridine-5-diphosphoglucose
This work was supported by the Deutsche Forschungsgemeinschaft (SFB 137). We are grateful to Professor E. Beck (Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, FRG) for providing laboratory facilities, and to Professor L. Willmitzer and Dr. U. Sonnewald (Institut für Genbiologische Forschung, Berlin, FRG) and Professor H.W. Heldt and Dr. D. Heineke (Institut für Biochemie der Pflanze, Universität Göttingen, FRG) for discussion. 相似文献
240.
Paolo Gasparini Anna Grifa Salvatore Savasta Ilaria Merlo Luigi Bisceglia Angela Totaro Leopoldo Zelante 《Human genetics》1994,94(6):671-674
The product of the human motilin gene (MLN) has an important role in regulating gastrointestinal motility. The precise chromosomal localisation and expression of this gene are still unresolved. Here, we report a detailed study assigning MLN to 6p21.3; MLN is tightly linked to the HLA-DQalpha locus. Moreover, MLN expression has been evaluated in a large series of tissues. Positive signals have been obtained for brain, bronchi and a gastrointestinal malignancy. Direct sequencing exon by exon of the codifying region, intron/exon boundaries and promoter has allowed the identification of three DNA polymorphisms, one of which corresponds to a common protein variant. The chromosomal localisation of MLN, and its expression in broncoepithelial cells suggests that this gene is involved in immotile-cilia syndrome (ICS) disease. Sequence and segregation analysis of the MLN gene carried out in two families, in which the disease locus was previously assigned to 6p21.3, exclude MLN as a candidate gene for the HLA-associated form of ICS. 相似文献