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201.
Berta Algueró Enrique Pedroso Vicente Marchán Anna Grandas 《Journal of biological inorganic chemistry》2007,12(6):901-911
Platinated oligonucleotides are promising tools for the control of gene expression, since they may target and cross-link nucleic
acid chains. Here we describe a method for the preparation of platinated oligonucleotides that has proved able to selectively
cross-link complementary sequences, making use of 5-methylcytidine analogs with thioether or imidazole groups attached to
the 4-position. These nucleoside analogs were derivatized as phosphoramidites and introduced in oligonucleotide chains using
standard phosphite triester chemistry. Different oligonucleotide sequences containing either one or two analogs appending
from the 5′-end were synthesized and used in preliminary platination studies. The reaction of transplatin with oligonucleotides
containing the thioether-modified nucleobase was fast, but generally afforded unstable adducts and complex reaction mixtures.
The imidazole-containing oligonucleotides reacted with transplatin much more slowly, in particular at slightly basic pH, and
it was found that the imidazole-modified cytosine was less reactive than the natural nucleobases. In contrast, transplatin
selectively reacted with the thioether and imidazole groups of oligonucleotides containing the two cytosine analogs in neighboring
positions, even in the presence of the four nucleobases and particularly three guanines, affording platinated oligonucleotides
suitable for cross-linking.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
202.
DNA and origin region segregation are not affected by the transition from rod to sphere after inhibition of Escherichia coli MreB by A22 总被引:1,自引:1,他引:0
Karczmarek A Martínez-Arteaga R Baselga RM Alexeeva S Hansen FG Vicente M Nanninga N den Blaauwen T 《Molecular microbiology》2007,65(1):51-63
The bacterial actin homologue MreB forms a helix underneath the cytoplasmic membrane and was shown to be essential in the morphogenesis of the rod-shaped cells. Additionally, MreB was implicated to be involved in DNA segregation. However, in our hands the mreBCD deletion strain (PA340-678) grew without apparent DNA segregation defect, suggesting that the reported chromosome segregation inhibition could be caused by a temporarily effect of MreB inhibition or depletion. To assess the involvement of MreB in DNA segregation during the transition from rod to sphere, we compared the effect of A22 and the PBP2 inhibitor mecillinam on the percentage of cells with segregated nucleoids and the number of oriC foci in wild-type Escherichia coli cells. Cells became spherical in the same time window during both treatments and we could not detect any difference in the chromosome or oriC segregation between these two treatments. Additionally, flow cytometric analyses showed that A22 and mecillinam treatment gave essentially the same chromosome segregation pattern. We conclude that MreB is not directly involved in DNA segregation of E. coli. 相似文献
203.
Sancho-Alonso María Taoro-Gonzalez Lucas Cabrera-Pastor Andrea Felipo Vicente Teruel-Martí Vicent 《Neurochemical research》2022,47(7):2016-2031
Neurochemical Research - Chronic hyperammonemia alters membrane expression of AMPA and NMDA receptors subunits in hippocampus leading to impaired memory and learning. Increasing extracellular cGMP... 相似文献
204.
The tomato res mutant which accumulates JA in roots in non‐stressed conditions restores cell structure alterations under salinity 下载免费PDF全文
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Javier Redondo-Mu?oz Vicente Pérez-García María J. Rodríguez José M. Valpuesta Ana C. Carrera 《Molecular and cellular biology》2015,35(1):249-263
The nuclear envelope (NE) forms a barrier between the nucleus and the cytosol that preserves genomic integrity. The nuclear lamina and nuclear pore complexes (NPCs) are NE components that regulate nuclear events through interaction with other proteins and DNA. Defects in the nuclear lamina are associated with the development of laminopathies. As cells depleted of phosphoinositide 3-kinase beta (PI3Kβ) showed an aberrant nuclear morphology, we studied the contribution of PI3Kβ to maintenance of NE integrity. pik3cb depletion reduced the nuclear membrane tension, triggered formation of areas of lipid bilayer/lamina discontinuity, and impaired NPC assembly. We show that one mechanism for PI3Kβ regulation of NE/NPC integrity is its association with RCC1 (regulator of chromosome condensation 1), the activator of nuclear Ran GTPase. PI3Kβ controls RCC1 binding to chromatin and, in turn, Ran activation. These findings suggest that PI3Kβ regulates the nuclear envelope through upstream regulation of RCC1 and Ran. 相似文献
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