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41.
42.
Tue Wenzel Kragstrup Babak Jalilian Kresten Krarup Keller Xianwei Zhang Julie Kristine Laustsen Kristian Stengaard-Pedersen Merete Lund Hetland Kim H?rslev-Petersen Peter Junker Mikkel ?stergaard Ellen-Margrethe Hauge Malene Hvid Thomas Vorup-Jensen Bent Deleuran 《PloS one》2016,11(2)
Introduction
In rheumatoid arthritis (RA) immune activation and presence of autoantibodies may precede clinical onset of disease, and joint destruction can progress despite remission. However, the underlying temporal changes of such immune system abnormalities in the inflammatory response during treat-to-target strategies remain poorly understood. We have previously reported low levels of the soluble form of CD18 (sCD18) in plasma from patients with chronic RA and spondyloarthritis. Here, we study the changes of sCD18 before and during treatment of early RA and following arthritis induction in murine models of rheumatoid arthritis.Methods
The level of sCD18 was analyzed with a time-resolved immunoflourometric assay in 1) plasma from early treatment naïve RA patients during a treat-to-target strategy (the OPERA cohort), 2) plasma from chronic RA patients, 3) serum from SKG and CIA mice following arthritis induction, and 4) supernatants from synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs) from 6 RA patients cultured with TNFα or adalimumab.Results
Plasma levels of sCD18 were decreased in chronic RA patients compared with early RA patients and in early RA patients compared with healthy controls. After 12 months of treatment the levels in early RA patients were similar to healthy controls. This normalization of plasma sCD18 levels was more pronounced in patients with very early disease who achieved an early ACR response. Plasma sCD18 levels were associated with radiographic progression. Correspondingly, the serum level of sCD18 was decreased in SKG mice 6 weeks after arthritis induction compared with healthy littermates. The sCD18 levels in both SKG and CIA mice exhibited a biphasic course after arthritis induction with an initial increase above baseline followed by a decline. Shedding of CD18 from RA SFMC and RA PBMC cultures was increased by TNFα and decreased by adalimumab.Conclusions
The plasma sCD18 levels were altered in patients with RA, in mice with autoimmune arthritis and in cell cultures treated with TNFα and adalimumab. Decreased levels of plasma sCD18 could reflect autoimmunity in transition from early to chronic disease and normalization in response to treatment could reflect autoimmunity in remission. 相似文献43.
Characterization of a human granulocyte differentiation antigen (CDw15) commonly recognized by monoclonal antibodies 总被引:1,自引:0,他引:1
Many different anti-human granulocyte monoclonal antibodies recognize the same carbohydrate antigen which contains the trisaccharide 3-fucosyl-N-acetyllactosamine. The antigen is expressed mainly on two cell surface glycoproteins of molecular weights around 105 K and 160 K which are apparently not members of the LFA-1 family of proteins. Although specific for granulocytes in blood, the antigen is expressed on a wide range of non-haemopoietic cell types. 相似文献
44.
YKL-40 protein expression in normal adult human tissues – an immunohistochemical study 总被引:1,自引:0,他引:1
Ringsholt M Høgdall EV Johansen JS Price PA Christensen LH 《Journal of molecular histology》2007,38(1):33-43
YKL-40, a 40 kDa plasma protein, is secreted by macrophages, neutrophils, chondrocytes, vascular smooth muscle cells and cancer
cells. High plasma YKL-40 is found in patients with inflammatory diseases and cancer, but it is not known how the protein
is expressed in tissues. This immunohistochemical study was carried out with the purpose of mapping and grading cytoplasmic
expression of YKL-40 in normal human tissue. Bovine serum albumin had to be used for pre-incubation in order to eliminate
background staining of YKL-40. The majority of cells were stained, but the intensity varied, not just among different cell
types but also within the same cell type. Cells known for exerting a high metabolic activity, i.e., high producing cells or
cells with high turn-over, tended to show the most intense cytoplasmic staining, which was weak or lacking in cells with no
or little activity. Many of these positive cells probably contribute to the YKL-40 found in plasma in healthy subjects in
accordance with previous findings on their in vitro production of the protein. In conclusion, all cells with a functioning
nucleus appeared to be capable of expressing YKL-40 in their cytoplasm, the intensity of which was dependent on cellular activity.
An erratum to this article can be found at 相似文献
45.
Tone Bjordal Johansen Ingrid Olsen Merete Rus?s Jensen Ulf R Dahle Gudmund Holstad Berit Dj?nne 《BMC microbiology》2007,7(1):14
Background
Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil, plants and bedding material. Isolates of M. avium originating from humans (n = 37), pigs (n = 51) and wild birds (n = 10) in Norway were examined by IS1245 and IS1311 RFLP using new and specific probes and for the presence of IS901 and ISMpa1 by PCR. Analysis and generation of a dendrogram were performed with the software BioNumerics. 相似文献46.
Pedersen M Jacobsen S Klarlund M Pedersen BV Wiik A Wohlfahrt J Frisch M 《Arthritis research & therapy》2006,8(4):R133-15
The aim of this study was to evaluate new and previously hypothesised non-genetic risk factors for serologic subtypes of rheumatoid
arthritis (RA) defined by the presence or absence of auto-antibodies to cyclic citrullinated peptides (CCP). In a national
case-control study, we included 515 patients recently diagnosed with RA according to the American College of Rheumatology
1987 classification criteria and 769 gender- and age-matched population controls. Telephone interviews provided information
about non-genetic exposures, and serum samples for patients were tested for anti-CCP-antibodies. Associations between exposure
variables and risk of anti-CCP-positive and anti-CCP-negative RA were evaluated using logistic regression. A series of RA
subtype-specific risk factors were identified. Tobacco smoking (odds ratio [OR] = 1.65; 95% confidence interval: 1.03–2.64,
for >20 versus 0 pack-years) was selectively associated with risk of anti-CCP-positive RA, whereas alcohol consumption exhibited
an inverse dose-response association with this RA subtype (OR = 1.98, 1.22–3.19, for 0 versus >0–5 drinks per week). Furthermore,
coffee consumption (OR = 2.18; 1.07–4.42, for >10 versus 0 cups per day), ever use of oral contraceptives (OR = 1.65; 1.06–2.57)
and having a first-degree relative with schizophrenia (OR = 4.18; 1.54–11.3) appeared more strongly associated with risk of
anti-CCP-positive RA. Obesity was selectively associated with risk of anti-CCP-negative RA, with obese individuals being at
more than 3-fold increased risk of this subtype compared with normal-weight individuals (OR = 3.45; 1.73–6.87). Age at menarche
was the only examined factor that was significantly associated with both serologic subtypes of RA (p-trends = 0.01); women
with menarche at age ≥ 15 years had about twice the risk of either RA subtype compared with women with menarche at age ≤ 12
years. Major differences in risk factor profiles suggest distinct etiologies for anti-CCP-positive and anti-CCP-negative RA. 相似文献
47.
Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum) 总被引:1,自引:0,他引:1
Common wheat (Triticum aestivum) has for decades been a textbook example of the evolution of a major crop species by allopolyploidization. Using a sophisticated extension of the PCR technique, we have successfully isolated two single-copy nuclear genes, DMC1 and EF-G, from each of the three genomes found in hexaploid wheat (BA(u)D) and from the two genomes of the tetraploid progenitor Triticum turgidum (BA(u)). By subjecting these sequences to phylogenetic analysis together with sequences from representatives of all the diploid Triticeae genera we are able for the first time to provide simultaneous and strongly supported evidence for the D genome being derived from Aegilops tauschii, the A(u) genome being derived from Triticum urartu, and the hitherto enigmatic B genome being derived from Aegilops speltoides. Previous problems of identifying the B genome donor may be associated with a higher diversification rate of the B genome compared to the A(u) genome in the polyploid wheats. The phylogenetic hypothesis further suggests that neither Triticum, Aegilops, nor Triticum plus Aegilops are monophyletic. 相似文献
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