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71.
Studying human impacts on riverine systems is challenging due to the natural diversity and multiple stressors that are co-occurring.
The term stressor(s) refers to variable(s) of anthropogenic landscape changes and local abiotic stream conditions that reflect human activities.
We compared the effects of different stressors and natural (typology) factors on benthic invertebrate assemblages in Slovenian rivers. A total of 270 river sites located in four European ecoregions
were sampled, covering the gradient of human perturbation ranging from (near)natural to highly degraded/polluted. Stressors
commonly affecting running waters were grouped into management-relevant stressor-groups: land use (percentage of urban, intensive and non-intensive agricultural, and natural land use in the catchment and sub-catchment),
eutrophication (in-stream concentrations of phosphorus and nitrogen compounds) and other stressors (e.g., organic pollution, hydromorphological alteration). We compared the effects of the stressor-groups on invertebrate
assemblages using partial Canonical Correspondence Analysis and tested the explanatory power of each stressor-group using
typology variables as covariates. Stressors and typology variables showed high independent effects (40–65%) on invertebrate assemblages despite considerable joint effects from land use and typology. Stressors’ pure effects accounted for 70%, and interactions among the stressor-groups made up the remaining 30% of the total
explained variability. Altogether, this study gives a good perspective on the ability to separate the effects of anthropogenic
stressors from natural causes, as well as to separate effects among groups of stressors. Against this background, we advocate
stressor-specific analyses to provide or improve guidelines for selecting appropriate measures to enhance the river status. 相似文献
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Anette?B?Grossi J?rgen?S?Agerholm Knud?Christensen Henrik?E?Jensen Páll?S?LeifssonEmail author Christian?Bendixen Peter?Karlskov-Mortensen Merete?Fredholm 《Acta veterinaria Scandinavica》2014,56(1):85
Background
Peripheral nerve sheath tumors (PNSTs) are frequently found in Danish cattle at slaughter. Bovine PNSTs share several gross and histopathological characteristics with the PNSTs in humans with heritable neurofibromatosis syndromes. The aim of the present study was to investigate a possible hereditary disposition to PNSTs in dairy cattle by statistical analysis performed on data from 567 cattle with PNSTs. Furthermore, a preliminary genome-wide association study (GWAS) was performed on DNA isolated from 28 affected and 28 non-affected Holstein cows to identify loci in the bovine genome involved in the development of PNSTs.Results
PNSTs were significantly more common in the Danish Holstein breed than in other breeds with 0.49% of Danish Holsteins slaughtered during an eight-year-period having PNSTs. PNSTs also occurred significantly more frequently in the offspring of some specific Holstein sires. Examination of three generation pedigrees showed that these sires were genetically related through a widely used US Holstein sire. The PNSTs included in GWAS were histologically classified as neurofibroma-schwannoma (43%), schwannoma (36%) and neurofibroma (21%) and derived from Holstein cows with multiple PNSTs. A single SNP on chromosome 27 reached genome-wide significance.Conclusions
Gross and histological characteristics of bovine PNSTs are comparable to PNSTs in humans (schwannomatosis). Danish Holsteins are genetically disposed to develop PNSTs but the examined materials are insufficient to allow determination of the mode of inheritance.74.
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Reduced heat shock response in human mononuclear cells during aging and its association with polymorphisms in HSP70 genes 总被引:3,自引:1,他引:3 下载免费PDF全文
Singh R Kølvraa S Bross P Jensen UB Gregersen N Tan Q Knudsen C Rattan SI 《Cell stress & chaperones》2006,11(3):208-215
Age-dependent changes in heat shock response (HSR) were studied in mononuclear cells (monocytes and lymphocytes) collected from young (mean age = 22.6 ± 1.7 years) and middle-aged (mean age = 56.3 ± 4.7 years) subjects after 1 hour of heat shock at 42°C. Genotype-specific HSR was measured by genotyping the subjects for 3 single nucleotide polymorphisms, HSPA1A(A-110C), HSPA1B(A1267G), and HSPA1L(T2437C), 1 each in the 3 HSP70 genes. A significant age-related decrease in the induction of Hsp70 occurred after heat shock in both monocytes and lymphocytes. The noninducible and inducible forms of Hsp70 decreased 1.3-fold (P < 0.001) and 1.4-fold (P < 0.001), respectively, in the monocytes with age. In the young subjects, a positive association was found between HSPA1L(T2437C) polymorphism and HSR. CC carriers had a significantly lower induction than TT carriers in both monocytes (P = 0.015) and lymphocytes (P = 0.044). This polymorphism, which is present in the coding region of HSPA1L gene, can affect the chaperoning function of Hsp70. These data consolidate our other observations that the CC genotype is unfavorable for human longevity and provide a functional explanation in terms of variations in HSR. 相似文献
77.
Shaji?KrishnanEmail author Henk?F. J.?Hendriks Merete?L.?Hartvigsen Albert?A.?de Graaf 《Theoretical biology & medical modelling》2016,13(1):17
Background
An artificial neural network approach was chosen to model the outcome of the complex signaling pathways in the gastro-intestinal tract and other peripheral organs that eventually produce the satiety feeling in the brain upon feeding.Methods
A multilayer feed-forward neural network was trained with sets of experimental data relating concentration-time courses of plasma satiety hormones to Visual Analog Scales (VAS) scores. The network successfully predicted VAS responses from sets of satiety hormone data obtained in experiments using different food compositions.Results
The correlation coefficients for the predicted VAS responses for test sets having i) a full set of three satiety hormones, ii) a set of only two satiety hormones, and iii) a set of only one satiety hormone were 0.96, 0.96, and 0.89, respectively. The predicted VAS responses discriminated the satiety effects of high satiating food types from less satiating food types both in orally fed and ileal infused forms.Conclusions
From this application of artificial neural networks, one may conclude that neural network models are very suitable to describe situations where behavior is complex and incompletely understood. However, training data sets that fit the experimental conditions need to be available.78.
Merete Grung Georg Kreimer Michael Calenberg Michael Melkonian Synnøve Liaaen-Jensen 《Planta》1994,193(1):38-43
Isolated intact eyespot apparatuses, the photoreceptive organelles involved in blue-light-mediated photoresponses of flagellate green algae, were analyzed regarding their carotenoid composition. Carotenoids from the eyespot apparatuses of Spermatozopsis similis were identified by high-performance liquid chromatography, visible-light absorption spectra, mass spectroscopy and by 1H-nuclear magnetic resonance spectroscopy (carotenes), and compared with those of whole-cell extracts. Both extracts contained ,-carotene, ,-carotene (formerly -carotene), lycopene, lutein, zeaxanthin, violaxanthin and all-E-and 9-Z-neoxanthin. The relative carotenoid compositions, however, differed significantly. A twofold relative increase in the total carotene level was evident in the fraction enriched in eyespot apparatuses. This was mainly due to an increase in the monocyclic ,-carotene and the aliphatic lycopene, whereas the relative content of ,-carotene remained unchanged. On the other hand a relative decrease in the total xanthophyll content, especially of lutein and the epoxidic carotenoid neoxanthin, was observed in the eyespot apparatuses compared with the whole-cell extracts. The decrease of the latter resulted almost solely from a reduction of the 9-Z-rather than the all-E-isomer. The bulk of the carotenes is thought to be localized in the highly organized eyespot lipid globules, which act as a combined quarter-wave interference reflector and absorption screen for the photoreceptor in green algae. The enrichment of ,-carotene and lycopene in the eyespot apparatuses, extending the range of visible light absorption to longer wavelengths, represents an adaptation of the screen to the retinal-based photoreceptor of flagellate green algae and is one of the prerequisites for maximal directional sensitivity of the eyespot apparatus.Abbreviations
1H-NMR
nuclear magnetic resonance
- IUPAC
International Union of Pure and Applied Chemistry
- VIS
visible absorption spectra
This work was supported by the Deutsche Forschungsgemeinschaft (G.K. and M.M.). M.G. was supported by a fellowship from the Norwegian Research Council of Science and Humanities. 相似文献
79.