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31.
Merav Chatzav Zvi Peleg Levent Ozturk Atilla Yazici Tzion Fahima Ismail Cakmak Yehoshua Saranga 《Annals of botany》2010,105(7):1211-1220
Background and Aims
Micronutrient malnutrition, particularly zinc and iron deficiency, afflicts over three billion people worldwide due to low dietary intake. In the current study, wild emmer wheat (Triticum turgidum ssp. dicoccoides), the progenitor of domesticated wheat, was tested for (1) genetic diversity in grain nutrient concentrations, (2) associations among grain nutrients and their relationships with plant productivity, and (3) the association of grain nutrients with the eco-geographical origin of wild emmer accessions.Methods
A total of 154 genotypes, including wild emmer accessions from across the Near Eastern Fertile Crescent and diverse wheat cultivars, were characterized in this 2-year field study for grain protein, micronutrient (zinc, iron, copper and manganese) and macronutrient (calcium, magnesium, potassium, phosphorus and sulphur) concentrations.Key Results
Wide genetic diversity was found among the wild emmer accessions for all grain nutrients. The concentrations of grain zinc, iron and protein in wild accessions were about two-fold greater than in the domesticated genotypes. Concentrations of these compounds were positively correlated with one another, with no clear association with plant productivity, suggesting that all three nutrients can be improved concurrently with no yield penalty. A subset of 12 populations revealed significant genetic variation between and within populations for all minerals. Association between soil characteristics at the site of collection and grain nutrient concentrations showed negative associations between soil clay content and grain protein and between soil-extractable zinc and grain zinc, the latter suggesting that the greatest potential for grain nutrient minerals lies in populations from micronutrient-deficient soils.Conclusions
Wild emmer wheat germplasm offers unique opportunities to exploit favourable alleles for grain nutrient properties that were excluded from the domesticated wheat gene pool. 相似文献32.
Roi Ben-David Weilong Xie Zvi Peleg Yehoshua Saranga Amos Dinoor Tzion Fahima 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(3):499-510
The gene-pool of wild emmer wheat, Triticum turgidum ssp. dicoccoides, harbors a rich allelic repertoire for disease resistance. In the current study, we made use of tetraploid wheat mapping
populations derived from a cross between durum wheat (cv. Langdon) and wild emmer (accession G18-16) to identify and map a
new powdery mildew resistance gene derived from wild emmer wheat. Initially, the two parental lines were screened with a collection
of 42 isolates of Blumeria graminis f. sp. tritici (Bgt) from Israel and 5 isolates from Switzerland. While G18-16 was resistant to 34 isolates, Langdon was resistant only to 5
isolates and susceptible to 42 isolates. Isolate Bgt#15 was selected to differentiate between the disease reactions of the two genotypes. Segregation ratio of F2-3 and recombinant inbreed line (F7) populations to inoculation with isolate Bgt#15 indicated the role of a single dominant gene in conferring resistance to Bgt#15. This gene, temporarily designated PmG16, was located on the distal region of chromosome arm 7AL. Genetic map of PmG16 region was assembled with 32 simple sequence repeat (SSR), sequence tag site (STS), Diversity array technology (DArT)
and cleaved amplified polymorphic sequence (CAPS) markers and assigned to the 7AL physical bin map (7AL-16). Using four DNA
markers we established colinearity between the genomic region spanning the PmG16 locus within the distal region of chromosome arm 7AL and the genomic regions on rice chromosome 6 and Brachypodium Bd1. A comparative analysis was carried out between PmG16 and other known Pm genes located on chromosome arm 7AL. The identified PmG16 may facilitate the use of wild alleles for improvement of powdery mildew resistance in elite wheat cultivars via marker-assisted
selection. 相似文献
33.
ITS2 sequences as barcodes for identifying and analyzing spider mites (Acari: Tetranychidae) 总被引:1,自引:0,他引:1
The use of DNA barcodes, short DNA sequences from a standardized region of the genome, has recently been proposed as a tool
to facilitate species identification and discovery. Here we show that second internal transcribed spacer of nuclear ribosomal
DNA (rDNA-ITS2) barcodes effectively discriminate among 16 species of spider mites (Acari: Tetranychidae) from Israel. The
barcode sequences of each species were unambiguously distinguishable from all other species and formed distinct, nonoverlapping
monophyletic groups in the maximum-parsimony tree. Sequence divergences were generally much greater between species than within
them. Using a 0.02 (2%) threshold for species diagnosis in our data set, 14 out of 16 species recognized by morphological
criteria would be accurately identified. The only exceptions involved the low divergence, 0.011–0.015 (1.1–1.5%), between
Tetranychus urticae and Tetranychus turkestani, where speciation may have occurred only recently. Still, these species had fixed alternative rDNA-ITS2 variants, with five
diagnostic nucleotide substitutions. As a result, we tentatively conclude that rDNA-ITS2 sequence barcodes may serve as an
effective tool for the identification of spider mite species and can be applicable as a diagnostic tool for quarantine and
other pest management activities and decision-making. We predict that our work, together with similar efforts, will provide
in the future the platform for a uniform, accurate, practical and easy-to-use method of spider mite species identification. 相似文献
34.
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36.
Oshrat Levy‐Ontman Merav Fisher Yoram Shotland Yoram Tekoah Shoshana Arad 《Journal of phycology》2015,51(6):1075-1087
N‐glycosylation of proteins is one of the most important post‐translational modifications that occur in various organisms, and is of utmost importance for protein function, stability, secretion, and loca‐lization. Although the N‐linked glycosylation pathway of proteins has been extensively characterized in mammals and plants, not much information is available regarding the N‐glycosylation pathway in algae. We studied the α 1,3‐glucosidase glucosidase II (GANAB) glycoenzyme in a red marine microalga Porphyridium sp. (Rhodophyta) using bioinformatic and biochemical approaches. The GANAB‐gene was found to be highly conserved evolutionarily (compo‐sed of all the common features of α and β subunits) and to exhibit similar motifs consistent with that of homolog eukaryotes GANAB genes. Phylogenetic analysis revealed its wide distribution across an evolutionarily vast range of organisms; while the α subunit is highly conserved and its phylogenic tree is similar to the taxon evolutionary tree, the β subunit is less conserved and its pattern somewhat differs from the taxon tree. In addition, the activity of the red microalgal GANAB enzyme was studied, including functional and biochemical characterization using a bioassay, indicating that the enzyme is similar to other eukaryotes ortholog GANAB enzymes. A correlation between polysaccharide production and GANAB activity, indicating its involvement in polysaccharide biosynthesis, is also demonstrated. This study represents a valuable contribution toward understanding the N‐glycosylation and polysaccharide biosynthesis pathways in red microalgae. 相似文献
37.
Cormode DP Skajaa GO Delshad A Parker N Jarzyna PA Calcagno C Galper MW Skajaa T Briley-Saebo KC Bell HM Gordon RE Fayad ZA Woo SL Mulder WJ 《Bioconjugate chemistry》2011,22(3):353-361
There are many liver diseases that could be treated with delivery of therapeutics such as DNA, proteins, or small molecules. Nanoparticles are often proposed as delivery vectors for such therapeutics; however, achieving nanoparticle accumulations in the therapeutically relevant hepatocytes is challenging. In order to address this issue, we have synthesized polymer coated, fluorescent iron oxide nanoparticles that bind and deliver DNA, as well as produce contrast for magnetic resonance imaging (MRI), fluorescence imaging, and transmission electron microscopy (TEM). The composition of the coating can be varied in a facile manner to increase the quantity of poly(ethylene glycol) (PEG) from 0% to 5%, 10%, or 25%, with the aim of reducing opsonization but maintaining DNA binding. We investigated the effect of the nanoparticle coating on DNA binding, cell uptake, cell transfection, and opsonization in vitro. Furthermore, we exploited MRI, fluorescence imaging, and TEM to investigate the distribution of the different formulations in the liver of mice. While MRI and fluorescence imaging showed that each formulation was heavily taken up in the liver at 24 h, the 10% PEG formulation was taken up by the therapeutically relevant hepatocytes more extensively than either the 0% PEG or the 5% PEG, indicating its potential for delivery of therapeutics to the liver. 相似文献
38.
Kizhner T Ben-David D Rom E Yayon A Livne E 《In vitro cellular & developmental biology. Animal》2011,47(4):294-301
Bone repair is a major concern in reconstructive surgery. Transplants containing osteogenically committed mesenchymal stem cells (MSCs) provide an alternative source to the currently used autologous bone transplants which have limited supply and require additional surgery to the patient. A major drawback, however is the lack of a critical mass of cells needed for successful transplantation. The purpose of the present study was to test the effects of FGF2 and FGF9 on expansion and differentiation of MSCs in order to establish an optimal culture protocol resulting in sufficient committed osteogenic cells required for successful in vivo transplantation. Bone marrow-derived MSCs cultured in αMEM medium supplemented with osteogenic supplements for up to three passages (control medium), were additionally treated with FGF2 and FGF9 in various combinations. Cultures were evaluated for viability, calcium deposition and in vivo osteogenic capacity by testing subcutaneous transplants in nude mice. FGF2 had a positive effect on the proliferative capacity of cultured MSCs compared to FGF9 and control medium treated cultures. Cultures treated with FGF2 followed by FGF9 showed an increased amount of extracted Alizarin red indicating greater osteogenic differentiation. Moreover, the osteogenic capacity of cultured cells transplanted in immunodeficient mice revealed that cells that were subjected to treatment with FGF2 in the first two passages and subsequently to FGF9 in the last passage only, were more successful in forming new bone. It is concluded that the protocol using FGF2 prior to FGF9 is beneficial to cell expansion and commitment, resulting in higher in vivo bone formation for successful bone tissue engineering. 相似文献
39.
L. Tsror M. Hazanovsky S. Mordechai-Lebiush T. Ben-David I. Dori E. Matan 《Journal of Phytopathology》2005,153(3):150-154
Control of root rot and wilt on Kangaroo Paw (Anigozanthos) caused by Pythium myriotylum was tested, using artificial inoculations, in greenhouse experiments. Disease incidence in the inoculated but non‐treated plots was 74% in Expt A (1999) and 46% in Expt B (2000). Disease incidence was significantly reduced by treatments with fenamidon (26 and 70%) and Terraclor SuperX (65 and 41%) in Expts A and B, respectively, in comparison with the non‐treated plots. Disease incidence was reduced with Ridomil Gold by 7 and 48% in Expts A and B, respectively; with Dynone by 22 and 41% in Expts A and B, respectively; with Tachigaren by 20% in both experiments; and with Trichoderma harzianum treatment, disease incidence was reduced by 9% only in Expt B. Yields (recorded only in Expt B) obtained in plots inoculated with P. myriotylum were significantly lower than in the non‐inoculated control plots. The most efficient treatment was fenamidon, with 324% more flowers than in the non‐treated control. The number of flowers in terraclor SuperX, Ridomil Gold, Dynone and T. harzianum treatments was higher by 285, 249, 234 and 235% than in the non‐treated control. In vitro tests demonstrated that the most effective doses, causing 50% inhibition of mycelial growth (ED50), were 0.09, 0.4, 0.4 and 4 μg/ml for the fungicides fenamidon, Ridomil Gold (mefenoxam), Terraclor SuperX (etridiazole + quintozene) and Dynone (prothiocarb), respectively. 相似文献
40.
Shao H Revach M Moshonov S Tzuman Y Gazit K Albeck S Unger T Dikstein R 《Molecular and cellular biology》2005,25(1):206-219
A major function of TFIID is core promoter recognition. TFIID consists of TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Most of them contain a histone fold domain (HFD) that lacks the DNA-contacting residues of histones. Whether and how TAF HFDs contribute to core promoter DNA binding are yet unresolved. Here we examined the DNA binding activity of TAF9, TAF6, TAF4b, and TAF12, which are related to histones H3, H4, H2A, and H2B, respectively. Each of these TAFs has intrinsic DNA binding activity adjacent to or within the HFD. The DNA binding domains were mapped to evolutionarily conserved and essential regions. Remarkably, HFD-mediated interaction enhanced the DNA binding activity of each of the TAF6-TAF9 and TAF4b-TAF12 pairs and of a histone-like octamer complex composed of the four TAFs. Furthermore, HFD-mediated interaction stimulated sequence-specific binding by TAF6 and TAF9. These results suggest that TAF HFDs merge with other conserved domains for efficient and specific core promoter binding. 相似文献