全文获取类型
收费全文 | 491277篇 |
免费 | 54648篇 |
国内免费 | 761篇 |
出版年
2018年 | 4857篇 |
2017年 | 4714篇 |
2016年 | 6806篇 |
2015年 | 9910篇 |
2014年 | 11393篇 |
2013年 | 15254篇 |
2012年 | 18127篇 |
2011年 | 18437篇 |
2010年 | 12058篇 |
2009年 | 10709篇 |
2008年 | 16064篇 |
2007年 | 16540篇 |
2006年 | 15608篇 |
2005年 | 14881篇 |
2004年 | 14858篇 |
2003年 | 13875篇 |
2002年 | 13537篇 |
2001年 | 20147篇 |
2000年 | 20186篇 |
1999年 | 16134篇 |
1998年 | 6033篇 |
1997年 | 5973篇 |
1996年 | 5687篇 |
1995年 | 5452篇 |
1994年 | 5158篇 |
1993年 | 5151篇 |
1992年 | 13132篇 |
1991年 | 13055篇 |
1990年 | 12803篇 |
1989年 | 12245篇 |
1988年 | 11402篇 |
1987年 | 10679篇 |
1986年 | 10211篇 |
1985年 | 9949篇 |
1984年 | 8282篇 |
1983年 | 7211篇 |
1982年 | 5478篇 |
1981年 | 4954篇 |
1980年 | 4566篇 |
1979年 | 7776篇 |
1978年 | 6299篇 |
1977年 | 5556篇 |
1976年 | 5185篇 |
1975年 | 6085篇 |
1974年 | 6594篇 |
1973年 | 6409篇 |
1972年 | 5771篇 |
1971年 | 5355篇 |
1970年 | 4500篇 |
1969年 | 4452篇 |
排序方式: 共有10000条查询结果,搜索用时 156 毫秒
941.
Calorimetric properties of mixtures of ganglioside GM1 and dipalmitoylphosphatidylcholine 总被引:2,自引:0,他引:2
L O Sillerud D E Schafer R K Yu W H Konigsberg 《The Journal of biological chemistry》1979,254(21):10876-10880
942.
943.
Liane Wehder Günther Ernst Anna C. Crecelius Orlando Guntinas-Lichius Christian Melle Ulrich S. Schubert Ferdinand von Eggeling 《The journal of histochemistry and cytochemistry》2010,58(10):929-937
Carcinoma tissue consists of not only tumor cells but also fibroblasts, endothelial cells or vascular structures, and inflammatory cells forming the supportive tumor stroma. Therefore, the spatial distribution of proteins that promote growth and proliferation in these complex functional units is of high interest. Matrix-assisted laser desorption/ionization imaging mass spectrometry is a newly developed technique that generates spatially resolved profiles of protein signals directly from thin tissue sections. Surface-enhanced laser desorption/ionization mass spectrometry (MS)combined with tissue microdissection allows analysis of defined parts of the tissue with a higher sensitivity and a broader mass range. Nevertheless, both MS-based techniques have a limited spatial resolution. IHC is a technique that allows a resolution down to the subcellular level. However, the detection and measurement of a specific protein expression level is possible only by semiquantitative methods. Moreover, prior knowledge about the identity of the proteins of interest is necessary. In this study, we combined all three techniques to gain highest spatial resolution, sensitivity, and quantitative information. We used frozen tissue from head and neck tumors and chose two exemplary proteins (HNP1–3 and S100A8) to highlight the advantages and disadvantages of each technique. It could be shown that the combination of these three techniques results in congruent but also synergetic data. (J Histochem Cytochem 58:929–937, 2010) 相似文献
944.
945.
946.
Most of the previous studies on the effects of iron deficiency on skeletal muscle respiratory capacity and work performance have been investigated in severe or moderate iron-deficiency anemia. We report here that even in mild iron deficiency where the hemoglobin concentration was 10 g/dl and the iron stores in livers and spleen were not completely depleted, a marked reduction in succinate dehydrogenase was observed in skeletal muscles but not in heart. Similarly, cytochrome oxidase activities were reduced. Although no significant change in glycerophosphate dehydrogenase was detected in the iron-deficient rats, exposure to cold in this group greatly reduced this enzyme activity. As cold acclimatization accelerates marrow erythropoiesis (20) which in turn, demands more iron, it seems that in the iron-insufficient state, this iron demand for marrow activity may persist at the expense of the tissue iron pool, resulting in a marked reduction in glycerophosphate dehydrogenase activities. Since succinate dehydrogenase plays a significant role in the impairment of mitochondrial function and early fatigue of iron-deficient muscle (11), the present study shows that even in mild iron deficiency, some loss of muscle functions could result as succinate dehydrogenase activities were greatly reduced. 相似文献
947.
948.
By freezing blocks of paraffin-embedded tissues to a convenient temperature it is possible to obtain routinely 1 micron sections that can be further processed as normal thicker sections. Normal and disposable steel knives can be used and the staining time should be increased in most procedures. Gradual freezing of blocks to the temperature of dry ice is the simplest and safest way to obtain an adequate temperature. The best results were obtained using as fixative 4% paraformaldehyde in phosphate buffered saline solution. 相似文献
949.
Summary The sex circle model for crossing over and gene conversion proposed by Stahl has a number of properties which are in conflict with a considerable body of recombination data in fungi. The model is unable to explain the observed frequencies of gene conversion and postmeiotic segregation for particular mutants in several species. It does not provide an explanation for fine structure map expansion, nor does it account satisfactorily for the polarised distribution of outside markers amongst allelic recombinants. 相似文献
950.