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931.
Using solid-phase organic synthesis, we have prepared a 432-member (18 x 8 x 3) sample library based on the AG 490 "tyrphostin" template. By utilizing 432 reactors each equipped with a unique radiofrequency memory ID tag, the 432 products could be obtained as discrete entities (i.e., not as mixtures) via 18 + 8 + 3, or 29 reactions. Reading each ID tag after each reaction step permitted the "directed sorting" of reactors into appropriate reaction vessels containing multiple reactors. After synthesis, all products were cleaved from the solid-phase support and lyophilized to afford powders. Characterization of 5% of the library members by NMR and mass spectrometry provided verification of structure. In addition, TLC analysis of every library member provided evidence that most (or all) are composed of a single major organic compound. Some 88% of these samples were obtained in amounts of between 5 and 19 mg. Using this reaction sequence and the "directed sorting" approach, the synthesis of much larger AG 490-based libraries can be envisioned. 相似文献
932.
Wild species are valued as a unique source of genetic variation, but they have rarely been used for the genetic improvement of quantitative traits. To identify trait-improving quantitative trait loci (QTL) alleles from exotic species, an accession of Oryza rufipogon, a relative of cultivated rice, was chosen on the basis of a genetic diversity study. An interspecific BC2 testcross population (V20A/O. rufipogon//V20B///V20B////Ce64) consisting of 300 families was evaluated for 12 agronomically important quantitative traits. The O. rufipogon accession was phenotypically inferior for all 12 traits. However, transgressive segregants that outperformed the original elite hybrid variety, V20A/Ce64, were observed for all traits examined. A set of 122 RFLP and microsatellite markers was used to identify QTL. A total of 68 significant QTL were identified, and of these, 35 (51%) had beneficial alleles derived from the phenotypically inferior O. rufipogon parent. Nineteen (54%) of these beneficial QTL alleles were free of deleterious effects on other characters. O. rufipogon alleles at two QTL on chromosomes 1 and 2 were associated with an 18 and 17% increase in grain yield per plant, respectively, without delaying maturity or increasing plant height. This discovery suggests that the innovative use of molecular maps and markers can alter the way geneticists utilize wild and exotic germplasm. 相似文献
933.
A yeast artificial chromosome (YAC) contig from the C57BL/6 (H2
b
) mouse was created from the major histocompatibility complex (Mhc, H2 in mouse) class Ib subregion, H2-M. It spans approximately 1.2 megabase (Mb) pairs and unites the previous >1.5-Mb YAC contigs (Jones et al. 1995) into a single
contig, which includes 21 Mhc class I genes distal to H2-T1. A bacterial artificial chromosome (BAC) contig from the 129 (H2
bc
) mouse, spanning approximately 600 kilobases, was also built from Znf173 (Afp, a gene for acid finger protein), through Tctex5 (t-complex testis expressed-5) and Mog (myelin oligodendrocyte glycoprotein), to H2-M2. Twenty-four sequence-tagged site (STS) markers were newly developed, and 35 markers were mapped in the YAC/BAC contigs,
which define the marker order as Cen –Znf173–Tctex5 – Mog–D17Tu42–D17Mit232–H2-M3–D17Leh525–H2-M2– Tel. The gene order of Znf173 – Tctex5 – Mog – D17Tu42 is conserved between mouse and human, showing that the middle H2-M region corresponds to the subregion of the human Mhc surrounding HLA-A.
Received: 25 July 1997 / Revised: 10 September 1997 相似文献
934.
935.
青蒿转杜松烯合成酶基因发根系的培养 总被引:10,自引:2,他引:8
将已克隆的棉花杜松烯合成酶的cDNA(cadC14)插入到植物表达载体pBI121中,构建含CaMV35S启动子驱动下的杜松烯合成酶基因的植物表达载体pBIC14。用含pBIC14质粒的发根农杆菌(Agrobacteriumrhizogenes)15834感染青蒿(ArtemisiaannuaL.)叶片并诱导发根,共建立121个生长迅速的发根系。经浓度为20mg/L的Kan筛选,获得12个抗Kan阳性根系。PCR和Southernbloting分析表明,外源杜松烯合成酶基因已整合到青蒿基因组中,其转基因频率为3%。RTPCR分析表明,外源杜松烯合成酶基因在C37根系中,在转录水平上已有表达。 相似文献
936.
Amy J. Pfizenmayer Matthew D. Vera Xiaobin Ding Dong Xiao Wei-Chuan Chen Madeleine M. Joullié Deepika Tandon Peter L. Toogood 《Bioorganic & medicinal chemistry letters》1998,8(24):752-3656
A didemnin B analog containing a Tic (1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) as a conformationally restrained replacement for tyrosine has been synthesized and shown to have comparable potency as a protein biosynthesis inhibitor. Synthetic highlights include an oxidation of an alcohol to an acid in the presence of the sensitive Tic heterocycle and a modified Schmidt-type one-pot macrocyclization. 相似文献
937.
草鱼和鲤群体遗传变异的RAPD指纹分析 总被引:30,自引:5,他引:25
利用随机扩增多态DNA技术对革鱼,兴国红鲤,野鲤的种群内,种群间以及种间的遗传变异亏待进行了定量分析。结果表明;草鱼与鲤的RAPD指纹图谱带型差异显著,草鱼与红鲤和鲤种间的平均带纹相似系数分别为0.2583和0.2394,遗传距离分别达到0.9362和1.2277。 相似文献
938.
森林旅游资源评价中的空气负离子研究 总被引:73,自引:2,他引:71
空气负离子被喻为空气维生素或生长素,对人体健康有利。自英国学者威尔逊与法国学者埃尔斯特和格特尔证实空气负离子的存在,人们对空气负离子的研究经历了近百年的发展、应用阶段1)。在我国,解放区战地医院曾采用了空气雾疗法,50年代有关文献中出现了空气负离子的... 相似文献
939.
940.
海蛇乙醇浸出物的营养成分分析 总被引:5,自引:2,他引:3
目的分析四种海蛇蛇体乙醇浸出物(AEBFSS)的营养成分。方法采用自动氨基酸分析仪,高效液相色谱仪和原子吸收分光光度仪。结果AEBFSS含有大量蛋白质和糖类物质;含有19种氨基酸和16种矿物元素。结论海蛇浸酒具有药用及营养功效。 相似文献