全文获取类型
收费全文 | 13013篇 |
免费 | 1117篇 |
国内免费 | 1322篇 |
专业分类
15452篇 |
出版年
2024年 | 52篇 |
2023年 | 240篇 |
2022年 | 535篇 |
2021年 | 855篇 |
2020年 | 592篇 |
2019年 | 678篇 |
2018年 | 646篇 |
2017年 | 497篇 |
2016年 | 582篇 |
2015年 | 909篇 |
2014年 | 997篇 |
2013年 | 1063篇 |
2012年 | 1286篇 |
2011年 | 1091篇 |
2010年 | 662篇 |
2009年 | 568篇 |
2008年 | 603篇 |
2007年 | 542篇 |
2006年 | 416篇 |
2005年 | 386篇 |
2004年 | 281篇 |
2003年 | 248篇 |
2002年 | 239篇 |
2001年 | 225篇 |
2000年 | 156篇 |
1999年 | 183篇 |
1998年 | 126篇 |
1997年 | 93篇 |
1996年 | 80篇 |
1995年 | 78篇 |
1994年 | 82篇 |
1993年 | 50篇 |
1992年 | 62篇 |
1991年 | 49篇 |
1990年 | 49篇 |
1989年 | 51篇 |
1988年 | 36篇 |
1987年 | 35篇 |
1986年 | 36篇 |
1985年 | 30篇 |
1984年 | 14篇 |
1983年 | 14篇 |
1982年 | 10篇 |
1981年 | 3篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1973年 | 2篇 |
1970年 | 2篇 |
1968年 | 2篇 |
1953年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
201.
202.
Assembly and maintenance of nodes of ranvier rely on distinct sources of proteins and targeting mechanisms 总被引:1,自引:0,他引:1
Zhang Y Bekku Y Dzhashiashvili Y Armenti S Meng X Sasaki Y Milbrandt J Salzer JL 《Neuron》2012,73(1):92-107
We have investigated the source(s) and targeting of components to PNS nodes of Ranvier. We show adhesion molecules are freely diffusible within the axon membrane and accumulate at forming nodes from local sources, whereas ion channels and cytoskeletal components are largely immobile and require transport to the node. We further characterize targeting of NF186, an adhesion molecule that pioneers node formation. NF186 redistributes to nascent nodes from a mobile, surface pool. Its initial accumulation and clearance from the internode require extracellular interactions, whereas targeting to mature nodes, i.e., those flanked by paranodal junctions, requires intracellular interactions. After incorporation into the node, NF186 is immobile, stable, and promotes node integrity. Thus, nodes assemble from two sources: adhesion molecules, which initiate assembly, accumulate by diffusion trapping via interactions with Schwann cells, whereas ion channels and cytoskeletal components accumulate via subsequent transport. In mature nodes, components turnover slowly and are replenished via transport. VIDEO ABSTRACT: 相似文献
203.
Supercapacitors: A Layered‐Nanospace‐Confinement Strategy for the Synthesis of Two‐Dimensional Porous Carbon Nanosheets for High‐Rate Performance Supercapacitors (Adv. Energy Mater. 7/2015) 下载免费PDF全文
204.
Lithium Batteries: Highly Nitridated Graphene–Li2S Cathodes with Stable Modulated Cycles (Adv. Energy Mater. 23/2015) 下载免费PDF全文
205.
206.
207.
Ga Hun Boo Ying‐Xiong Qiu Jung Yeon Kim Put O. Ang Samuel Bosch Olivier De Clerck Peimin He Atsushi Higa Bangqin Huang Kazuhiro Kogame Shao‐Lun Liu Tu van Nguyen Shoichiro Suda Ryuta Terada Kathy Ann Miller Sung Min Boo 《Journal of phycology》2019,55(6):1319-1334
The evolutionary and population demographic history of marine red algae in East Asia is poorly understood. Here, we reconstructed the phylogeographies of two upper intertidal species endemic to East Asia, Gelidiophycus divaricatus and G. freshwateri. Phylogenetic and phylogeographic inferences of 393 mitochondrial cox1, 128 plastid rbcL, and 342 nuclear ITS2 sequences were complemented with ecological niche models. Gelidiophycus divaricatus, a southern species adapted to warm water, is characterized by a high genetic diversity and a strong geographical population structure, characteristic of stable population sizes and sudden reduction to recent expansion. In contrast, G. freshwateri, a northern species adapted to cold temperate conditions, is genetically relatively homogeneous with a shallow population structure resulting from steady population growth and recent equilibrium. The overlap zone of the two species roughly matches summer and winter isotherms, indicating that surface seawater temperature is a key feature influencing species range. Unidirectional genetic introgression was detected at two sites on Jeju Island where G. divaricatus was rare while G. freshwateri was common, suggesting the occurrence of asymmetric natural hybrids, a rarely reported event for rhodophytes. Our results illustrate that Quaternary climate oscillations have left strong imprints on the current day genetic structure and highlight the importance of seawater temperature and sea level change in driving speciation in upper intertidal seaweed species. 相似文献
208.
Meng Zhang Xinkai Ding Weixiang Peng Hongliang Zhang Bingjie Wu 《Molecular simulation》2019,45(3):178-185
Radiation damage in reactor materials caused by the collision of the fast neutrons has a great impact on the reliability and safety of nuclear reactors. The element vanadium has attracted interest in many fields due to its advantageous properties in alloys. Thus, molecular dynamics simulation (MD) and first-principles calculation have been executed here to explore the radiation-resistant properties of five materials adding a layer in the bulk (pure iron and four types of Fe–V alloys containing 10%-40% V). The following results were inferred from these simulations. Firstly, the number of Frenkel pairs (FPs) at the stable quenching stage in the bulk decreases when the Fe–V alloy is added as an anti-radiation layer to the bulk. These benefits are evident for the Fe80V20 and alloy layers with more vanadium. The main reason is that the Fe–V binding energy is greater than the Fe-Fe binding energy, which can make the Primary Knock-On atom (PKA) lose more energy at the Fe–V alloy layer. Secondly, the average value of point-defect, cluster and defect clustered fractions in the bulk of Fe–V alloy is smaller than that in the pure iron at the stable quenching stage, especially for the Fe80V20 alloy. 相似文献
209.
Tao Zhong Jianmei Zhang Xiaoying Han Xiaoxiao Gongye Tianqi Lyu Menghan Jiang Kaiwei Yu Xiaoqian Meng Dong Cheng Hui Lyu Tianliang Zhang Lei Zhang Shuzhen Liu 《Journal of cellular biochemistry》2019,120(9):15337-15346
3,3′,4,4′,5-Polychlorinated biphenyl (PCB126) is a persistent organic environmental pollutant which can affect various biological activities of organisms, such as immunity, neurological function, and reproduction. In our study, we aimed to investigate the effects of PCB126 on granulosa cells (GCs). GCs were collected from ovaries in PMSG-treated mice, after 24 hours culture. GCs were then incubated with 10 pg/mL, 100 pg/mL, and 10 ng/mL of PCB126 for another 24 hours. Following these steps, exposed GCs were collected for further experimentation. Our data showed that the number of GCs in the 10 ng/mL PCB126 decreased. Meanwhile, pyknotic nuclei and condensed chromatin increased, while the apoptotic cells in the 10 ng/mL PCB126 group were significantly increased. Furthermore, the expression of the apoptotic executive protein caspase-3 increased after PCB126 treatment. The expression of Bax, Bcl-2, and Bim related to the mitochondrial apoptosis pathway were also influenced to different degrees. Thus, our data suggested that PCB126 affect the GCs apoptosis, and mitochondrial apoptosis pathway was involved in this process. 相似文献
210.
Information on the spatial distribution of cytotypes and karyotype variation in plants is critical for studies of the origin and evolution of polyploid complexes. Here, the spatial distribution of cytological races and intraspecific variation in the karyotype of Lycoris radiata, an endemic species to East Asia, is investigated. Conventional karyotype analysis methods were used to determine ploidy level and karyotypical characteristics in 2,420 individuals from 114 populations of L. radiata nearly covering the whole distribution areas in China. Of 114 populations studied, 52 (45.61%), 58 (50.88%), and 4 (3.51%) are diploid, triploid, and mixoploid populations, respectively, with 1,224, 1,195, and 1 individuals being diploid, triploid, and tetraploid, respectively. The triploid possesses a much wider distribution range than the diploid, with the former almost occupying the entire range of this complex species in East Asia and the latter distributing in the middle and east regions of China. Triploids tend to occur at high altitudes, and the relationship between the ploidy and altitude is significantly positive but low (r2 = 0.103, p < 0.01). About 98.6% of examined bulbs have a common karyotype consisting of 22 or 33 acrocentric (A) chromosomes. Some aberrant chromosomes which should be generated from A‐type chromosome have been found including metacentrics (m), small metacentrics (m′), and B‐type chromosome. The results can provide a fundamental cytogeographic data for further studies on the evolutionary origins and adaptive divergences of polyploids, especially the triploid, within L. radiata using molecular and/or ecological methods in the future. 相似文献