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81.
Bezerra GA Oliveira TM Moreno FB de Souza EP da Rocha BA Benevides RG Delatorre P de Azevedo WF Cavada BS 《Journal of structural biology》2007,160(2):168-176
Plant lectins, especially those purified from species of the Leguminosae family, represent the best studied group of carbohydrate-binding proteins. The legume lectins from Diocleinae subtribe are highly similar proteins that present significant differences in the potency/efficacy of their biological activities. The structural studies of the interactions between lectins and sugars may clarify the origin of the distinct biological activities observed in this high similar class of proteins. In this way, this work presents a crystallographic study of the ConM and CGL (agglutinins from Canavalia maritima and Canavalia gladiata, respectively) in the following complexes: ConM/CGL:Man(alpha1-2)Man(alpha1-O)Me, ConM/CGL:Man(alpha1-3)Man(alpha1-O)Me and ConM/CGL:Man(alpha1-4)Man(alpha1-O)Me, which crystallized in different conditions and space group from the native proteins. The structures were solved by molecular replacement, presenting satisfactory values for R(factor) and R(free). Comparisons between ConM, CGL and ConA (Canavalia ensiformis lectin) binding mode with the dimannosides in subject, presented different interactions patterns, which may account for a structural explanation of the distincts biological properties observed in the lectins of Diocleinae subtribe. 相似文献
82.
83.
do Nascimento AM Costa FC Thiemann OH de Oliveira DC 《Zeitschrift für Naturforschung. C, Journal of biosciences》2007,62(5-6):353-356
The dichloromethane extract of Calea uniflora afforded a mixture of two novel chromanones, uniflorol-A (1) and uniflorol-B (2), and one known chromanone, 2,2-dimethyl-6-(1-hydroxyethyl)-chroman-4-one (3). The structures of these compounds were determined by spectroscopic methods. Biological activity of the compounds against Leishmania major promastigotes was evaluated. Mixture of the novel chromanones 1 and 2 showed significant growth inhibition of the parasite in the micrograms per milliliter range. 相似文献
84.
Mendes KN Nicorici D Cogdell D Tabus I Yli-Harja O Guerra R Hamilton SR Zhang W 《Journal of proteome research》2007,6(7):2753-2767
Multiple signal transduction pathways play a crucial role in cancer development, progression, and response to different therapies. An important issue is whether common signal transduction pathways are ubiquitously altered in all cancer types and some unique pathways are involved in different cancer types. Another important issue is whether and how transduction signaling molecules are heterogeneously expressed and activated in different cancer cells within and between cancer cell types. METHODS: To gain insight into these issues, we assembled a protein lysate array with 90 different cell lines of 12 different cell types. Each sample is diluted 2-fold six times, and samples from the dilution series were printed three times on the array. We then measured the expression levels and phosphorylation status of 52 different signaling proteins with specific antibodies and carried out statistical hierarchical clustering analysis. RESULTS: The most significant finding based on the cluster analysis was that the cell lines did not group based on tumor types, suggesting that the signaling pathways studied were commonly activated in most of the tumor types cultured in vitro. As expected, related proteins associated with specific signaling pathways clustered together, and analysis of the 30 most differentially expressed proteins revealed the PI3-K signaling pathway was upregulated in several different tumor types and the VEGF-angiogenesis pathway was downregulated in hematopoetic cancers. Another important observation, with clinical implications was that EGFR was the most heterogeneous among all the cell lines. We also observed signaling pathways unique to specific types of cancers such as the inverse relationship between p16ink and Rb, and the EGFR mediated pathway activation characteristic of pancreatic cancers. CONCLUSIONS: Using reverse phase lysate array analysis in this study, we were able to determine potential relationships and signaling pathways, both common and unique, to different types of cancer using cell lines in vitro. This data could be utilized for mining information related to an individual cancer of interest and combined with morphological and genomic profiles would help in creating a combination of expression markers and/or functional signaling maps for specific cancer diagnosis and therapy. 相似文献
85.
Piccinini Alexandre Oliveira Mariana Pacheco Silva Mariella Reinol Bett Gabriela Souza Becker Isabel Borges Mendes Talita Farias Salla Daniéle Hendler Silva Larissa Espindola Vilela Thais Ceresér Moraes Fernanda Mendes Moterle Diego Damiani Adriani Paganini Dagostin Lígia Salvan Tietbohl Lariani Tamires Bittencourt João Vitor Silvano Biehl Erica Denicol Tais Luise Bonfante Sandra Regina Andrade Vanessa Moraes Silveira Paulo Cesar Lock Prophiro Josiane Somariva Ferreira Gabriela Kozuchovski Petronilho Fabricia Kanis Luiz Alberto Rezin Gislaine Tezza 《Neurochemical research》2022,47(7):1888-1903
Neurochemical Research - This study aimed to evaluate the effect of Cynara cardunculus leaf ethanol extract on inflammatory and oxidative stress parameters in the hypothalamus, prefrontal cortex,... 相似文献
86.
87.
The galactose‐binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines
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Deysen Kerlla Fernandes Bezerra Girão Benildo Sousa Cavada Alana de Freitas Pires Timna Varela Martins Álvaro Xavier Franco Cecília Mendes Morais Kyria Santiago do Nascimento Plinio Delatorre Helton Colares da Silva Celso Shiniti Nagano Ana Maria Sampaio Assreuy Pedro Marcos Gomes Soares 《Journal of molecular recognition : JMR》2015,28(5):285-292
In this study, the amino acid sequence and anti‐inflammatory effect of Bauhinia bauhinioides (BBL) lectin were evaluated. Tandem mass spectrometry revealed that BBL possesses 86 amino acid residues. BBL (1 mg/kg) intravenously injected in rats 30 min prior to inflammatory stimuli inhibited the cellular edema induced by carrageenan in only the second phase (21% – 3 h, 19% – 4 h) and did not alter the osmotic edema induced by dextran. BBL also inhibited carrageenan peritoneal neutrophil migration (51%), leukocyte rolling (58%) and adhesion (68%) and the neutrophil migration induced by TNF‐α (64%). These effects were reversed by the association of BBL with galactose, demonstrating that the carbohydrate‐binding domain is essential for lectin activity. In addition, BBL reduced myeloperoxidase activity (84%) and TNF‐α (68%) and IL1‐β (47%) levels. In conclusion, the present investigation demonstrated that BBL contains highly homologous isolectins, resulting in a total of 86 amino acid residues, and exhibits anti‐inflammatory activity by inhibiting neutrophil migration by reducing TNF‐α and IL1‐β levels via the lectin domain. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
88.
89.
Marco Aurélio Soares Roberta Amália de Carvalho Araújo Marjorie Mendes Marini Luciana Márcia de Oliveira Leonardo Gomes de Lima Viviane de Souza Alves Maria Sueli Soares Felipe Marcelo Macedo Brigido Celia Maria de Almeida Soares Jose Franco da Silveira Jeronimo Concei??o Ruiz Patrícia Silva Cisalpino 《BMC genomics》2015,16(1)
Background
Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes.Results
We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR.Conclusion
Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1564-7) contains supplementary material, which is available to authorized users. 相似文献90.
Review of Florida Red Tide and Human Health Effects 总被引:1,自引:0,他引:1
Fleming LE Kirkpatrick B Backer LC Walsh CJ Nierenberg K Clark J Reich A Hollenbeck J Benson J Cheng YS Naar J Pierce R Bourdelais AJ Abraham WM Kirkpatrick G Zaias J Wanner A Mendes E Shalat S Hoagland P Stephan W Bean J Watkins S Clarke T Byrne M Baden DG 《Harmful algae》2011,10(2):224-233
This paper reviews the literature describing research performed over the past decade on the known and possible exposures and human health effects associated with Florida red tides. These harmful algal blooms are caused by the dinoflagellate, Karenia brevis, and similar organisms, all of which produce a suite of natural toxins known as brevetoxins. Florida red tide research has benefited from a consistently funded, long term research program, that has allowed an interdisciplinary team of researchers to focus their attention on this specific environmental issue-one that is critically important to Gulf of Mexico and other coastal communities. This long-term interdisciplinary approach has allowed the team to engage the local community, identify measures to protect public health, take emerging technologies into the field, forge advances in natural products chemistry, and develop a valuable pharmaceutical product. The Review includes a brief discussion of the Florida red tide organisms and their toxins, and then focuses on the effects of these toxins on animals and humans, including how these effects predict what we might expect to see in exposed people. 相似文献