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991.
Ana Luiza Tardem Maciel Thayana da Conceio Barbosa Caroline Barbieri Blunck Karolyne Wolch Amanda de Albuquerque Lopes Machado Elaine Sobral da Costa Lavínia Lustosa Bergier Mrcia Trindade Schramm Maura Rosane Valrio Ikoma-Coltutato Mecneide Mendes Lins Thais Ferraz Aguiar Marcela Braga Mansur Mariana Emerenciano 《Translational oncology》2022,15(1)
Cytokine Receptor-Like Factor 2 (CRLF2) overexpression occurs in 5-15% of B-cell precursor acute lymphoblastic leukaemia (B-ALL). In ∼50% of these cases, the mechanisms underlying this dysregulation are unknown. IKAROS Family Zinc Finger 1 (IKZF1) is a possible candidate to play a role in this dysregulation since it binds to the CRLF2 promoter region and suppresses its expression. We hypothesised that IKZF1 loss of function, caused by deletions or its short isoforms expression, could be associated with CRLF2 overexpression in B-ALL. A total of 131 paediatric and adult patients and 7 B-ALL cell lines were analysed to investigate the presence of IKZF1 deletions and its splicing isoforms expression levels, the presence of CRLF2 rearrangements or mutations, CRLF2 expression and JAK2 mutations. Overall survival analyses were performed according to the CRLF2 and IKZF1 subgroups. Our analyses showed that 25.2% of patients exhibited CRLF2 overexpression (CRLF2-high). CRLF2-high was associated with the presence of IKZF1 deletions (IKZF1del, p = 0.001), particularly with those resulting in dominant-negative isoforms (p = 0.006). Moreover, CRLF2 expression was higher in paediatric samples with high loads of the short isoform IK4 (p = 0.011). It was also associated with the occurrence of the IKZF1 plus subgroup (p = 0.004). Furthermore, patients with CRLF2-high/IKZF1del had a poorer prognosis in the RELLA05 protocol (p = 0.067, 36.1 months, 95%CI 0.0-85.9) and adult cohort (p = 0.094, 29.7 months, 95%CI 11.8–47.5). In this study, we show that IKZF1 status is associated with CRLF2-high and dismal outcomes in B-ALL patients regardless of age. 相似文献
992.
993.
de Menezes Luiz Henrique Sales do Esprito Santo Eliezer Luz dos Santos Marta Maria Oliveira de Carvalho Tavares Iasnaia Maria Mendes Adriano Aguiar Franco Marcelo de Oliveira Julieta Rangel 《Biotechnology letters》2022,44(1):89-99
Biotechnology Letters - To immobilize Candida rugosa lipase in Accurel MP 1000 (CRL-AMP) by physical adsorption in organic medium and apply in the synthesis of wax esters dodecanoyl octadecanoate 1... 相似文献
994.
de Oliveira Carvalho Andra da Consolao Kerr Rodrigo Tavano Virginia Maria Mendes Carlos Rafael B. 《Biogeochemistry》2022,159(2):139-158
Biogeochemistry - The structure of the phytoplankton community is strongly influenced by environmental variables linked with variations in sea–air CO2 net fluxes (FCO2). However, compared to... 相似文献
995.
A new species of spore, Costatoperforosporites friisiae sp. nov., is described from the Early Cretaceous (late Aptian–early Albian) deposits of Catefica in the Lusitanian Basin, western Portugal. Although the morphology of the muri is clearly of the Cicatricosisporites type, the presence of micropores on these sculptural elements and within the intervening grooves is a particularly distinctive feature and more typical of spores that have been attributed previously to Costatoperforosporites. As a result, this genus is emended not only to accommodate Costatoperforosporites friisiae but also to differentiate it more clearly from other, similar, murornate genera. It is considered to represent the family Anemiaceae. So far, Costatoperforosporites friisiae has only been encountered in the Catefica palynoflora. The presence of many other pteridophyte spores, especially of schizaealean derivation, together with abundant cheirolepidiacean remains at this locality, strongly suggests a warm, moist climate and diverse source vegetation. 相似文献
996.
997.
Ecology and identification of environmental fungi and metabolic processes involved in the biodeterioration of Brazilian soapstone historical monuments 下载免费PDF全文
998.
Andersom Milech Einhardt Sandro Ferreira Lillian Mathias Oliveira Dimas Mendes Ribeiro Fabrício Ávila Rodrigues 《Physiologia plantarum》2020,170(4):592-606
Nickel (Ni) and glyphosate (Gl) are able to reduce the symptoms of Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, in soybean. However, their combined effects on the energy balance and ethylene metabolism of soybean plants infected with this fungus has not been elucidated. Therefore, the effects of Ni, Gl, and the combination of Ni + Gl on ASR development, photosynthetic capacity, sugar concentrations, and ethylene concentrations in plants of a Gl-resistant cultivar, uninfected or infected with P. pachyrhizi, were investigated. Inoculated plants supplied with Ni had the highest foliar Ni concentration in all the treatments. Gl had a negative effect on the foliar Ni concentration in Ni-sprayed plants. The ASR severity was reduced in plants sprayed with Ni and Gl. Carotenoid and chlorophyll concentrations were higher in inoculated Ni, Gl, and Ni + Gl plants than in control plants. Based on the chlorophyll a fluorescence parameters, the photosynthetic apparatus of the control inoculated plants was damaged, and the least amount of energy was directed to the photochemistry process in these plants. The reduced capacity of the photosynthetic mechanism to capture light and use the energy absorbed by photosystem II in inoculated plants was reflected in their reduced capacity to process CO2, as indicated by the high internal CO2 concentrations and low rates of net carbon assimilation. The low sugar concentrations in inoculated plants from the control treatment were linked to their reduced photosynthetic capacity due to the high ASR severity. In uninfected plants, the ethylene concentration was not affected by Ni or Gl, while the ethylene concentration decreased in inoculated plants; this decrease was more pronounced in plants from the control treatment than in treated inoculated plants. In conclusion, this study sheds light on the role played by both Ni and Gl in ASR control from a physiological perspective. Soybean plants exposed to Ni and Gl were able to maintain high ethylene concentrations and photosynthetic capacity during the P. pachyrhizi infection process; as a result, these plants consumed less of their reserves than inoculated plants not treated with Ni or Gl. 相似文献
999.
1000.
Márcio L. Tomaz Beatriz M. Januzzi Mendes francisco De Assis A. Mourão Filho Clarice G. B. Demétrio Naratip Jansakul Adriana P. Martinelli Rodriguez 《In vitro cellular & developmental biology. Plant》2001,37(4):446-452
Summary Somatic embryogenesis from nucellus-derived callus cultures of five cultivars, including three (Caipira, Seleta Vermelha,
and Valencia) of sweet oranges (C. sinesis L. Osbeck), Rangpur lime (C. limonia L. Osbeck), and Cleopatra mandarin (C. reticulata Blanco) (lines I and II), were studied. Callus lines maintained on MT medium supplemented with 50 g l−1 sucrose were transferred to MT medium supplemented with different carbohydrate sources: galactose, glucose, lactose, maltose,
or sucrose at 18, 37, 75, 110, or 150 mM, or glycerol at 6, 12, 24, 36, or 50 mM. Globular embryos were observed after approximately 4 wk, in several treatments. Cultures of Valencia and Caipira sweet oranges
and Cleopatra mandarin (line I) showed high numbers of embryos on medium containing galactose, lactose, and maltose. Histological
studies showed somatic embryos in all developmental stages with a normal histodiffeentiation pattern. The other two cultivars
(Rangpur lime and Cleopatra mandarin, line II) formed very few embryos, which did not develop further following the globular
stage. Some of the abnormalities observed were lack or dedifferentiation of protoderm and absence of apical meristems and
procambial strands. Embryos that followed the normal sequence of development were easily converted into plants. Non-embryogenic
cultures continued as proliferating callus cultures, eventually forming a few embryos which did not convert into plants. Statistical
analyses of the callus response to carbohydrate treatments was done using an overdispersion Poisson model. 相似文献