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311.
Tracing the origin of a cryptic invader: phylogeography of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex
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Richard Stouthamer Paul Rugman‐Jones Pham Q. Thu Akif Eskalen Tim Thibault Jiri Hulcr Liang‐Jong Wang Bjarte H. Jordal Chi‐Yu Chen Miriam Cooperband Ching‐Shan Lin Naoto Kamata Sheng‐Shan Lu Hayato Masuya Zvi Mendel Robert Rabaglia Sunisa Sanguansub Hsin‐Hui Shih Wisut Sittichaya Shixiang Zong 《Agricultural and Forest Entomology》2017,19(4):366-375
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312.
Daniel Kleiner Ziva Shapiro Tuchman Fannia Shmulevich Anat Shahar Raz Zarivach Mickey Kosloff Shimon Bershtein 《Protein science : a publication of the Protein Society》2022,31(7)
Homomers are prevalent in bacterial proteomes, particularly among core metabolic enzymes. Homomerization is often key to function and regulation, and interfaces that facilitate the formation of homomeric enzymes are subject to intense evolutionary change. However, our understanding of the molecular mechanisms that drive evolutionary variation in homomeric complexes is still lacking. How is the diversification of protein interfaces linked to variation in functional regulation and structural integrity of homomeric complexes? To address this question, we studied quaternary structure evolution of bacterial methionine S‐adenosyltransferases (MATs)—dihedral homotetramers formed along a large and conserved dimeric interface harboring two active sites, and a small, recently evolved, interdimeric interface. Here, we show that diversity in the physicochemical properties of small interfaces is directly linked to variability in the kinetic stability of MAT quaternary complexes and in modes of their functional regulation. Specifically, hydrophobic interactions within the small interface of Escherichia coli MAT render the functional homotetramer kinetically stable yet impose severe aggregation constraints on complex assembly. These constraints are alleviated by electrostatic interactions that accelerate dimer‐dimer assembly. In contrast, Neisseria gonorrhoeae MAT adopts a nonfunctional dimeric state due to the low hydrophobicity of its small interface and the high flexibility of its active site loops, which perturbs small interface integrity. Remarkably, in the presence of methionine and ATP, N. gonorrhoeae MAT undergoes substrate‐induced assembly into a functional tetrameric state. We suggest that evolution acts on the interdimeric interfaces of MATs to tailor the regulation of their activity and stability to unique organismal needs. 相似文献
313.
Radiation inactivation of binding sites for high density lipoproteins in human fibroblast membranes 总被引:2,自引:0,他引:2
C M Mendel S T Kunitake J P Kane E S Kempner 《The Journal of biological chemistry》1988,263(3):1314-1319
Radiation inactivation and target analysis were used to determine the molecular mass of the binding sites for high density lipoproteins (HDL) on membranes prepared from human fibroblasts. These membrane binding sites shared characteristics with the previously described HDL binding sites on whole fibroblasts in tissue culture. They exhibited the same affinity for HDL, the same ligand specificity, and the same sensitivity to proteolytic agents. They were also up-regulated by cholesterol loading of the cells. Kinetics of HDL dissociation from membrane binding sites could not be described by a single exponential function, indicating that HDL probably bind to multiple classes of sites on fibroblast membranes. After exposure to ionizing radiation, these sites decreased in number as an apparent single exponential function of radiation dose, corresponding to an average molecular mass of 16,000 +/- 1,000 Da, which is smaller than any known cell-surface receptor protein. These data indicate that HDL binding sites on fibroblast membranes are not "classical" receptors in that they are kinetically heterogeneous and small in molecular mass. 相似文献
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Evidence is presented for the presence of nitrite reductasein citrus leaves. The enzyme has a Km for nitrite of 45 mu andis inhibited by cyanide. However, unlike citrus nitrate reductase(l), it is probably not a metalloflavo protein, although itmay be related to iron. In addition to the enzymatic nitrite reduction, non-enzymaticnitrite reduction was present in citrus leaf preparations. Underin vivo assay conditions nitrite reduction in one-month-oldleaves was not inhibited by cyanide, in contrast with three-month-oldleaves in which nitrite reduction was almost completely inhibited.Thus it appears that in very young citrus leaves most of thenitrite reduction is non-enzymatic.
1 Contribution from The Volcani Center, Agricultural ResearchOrganization, P. O. B. 6, Bet Dagan, Israel. Series 1972.........2256AE. (Received November 28, 1972; ) 相似文献
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Seven aromatic metabolites of phenylalanine were determined in plasma of 20 patients with classical phenylketonuria by means of capillary gas chromatography. The results obtained showed good correlation with plasma phenylalanine levels. Plasma aromatic acid levels may prove useful in the diagnosis and management of phenylketonuria, as well as in research of this disorder. 相似文献
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C M Mendel S T Kunitake K Hong S K Erickson J P Kane E S Kempner 《Biochimica et biophysica acta》1988,961(2):188-193
High-density lipoproteins (HDL) are involved in 'reverse cholesterol transport'. Whether or not cell-surface receptors for HDL exist and participate in this process remains controversial, and part of this controversy has centered on the nature of the HDL binding sites. We therefore used radiation inactivation to determine the molecular mass of the HDL binding sites in human liver membranes in situ. These binding sites, which shared all the characteristics of previously described putative HDL receptors, had a molecular mass of less than 10 kDa, indicating that they are probably not proteins. In addition, the binding of HDL to protein-free liposomes was characterized and was found to display the same affinity (KD = 5 micrograms protein/ml approximately 5.10(-8) M) as that to cell membranes, indicating that HDL binding to cell membranes may not require membrane proteins. These observations highlight an important application of radiation inactivation: the ability to demonstrate that something - in this case, a high-molecular-weight protein that accounts for the majority of the HDL binding activity in human liver membranes - is absent. 相似文献